التفاصيل البيبلوغرافية
| العنوان: |
Septins and K63 ubiquitin chains are present in separate bacterial microdomains during autophagy of entrapped Shigella. |
| المؤلفون: |
Lobato-Márquez, Damián, Conesa, José Javier, López-Jiménez, Ana Teresa, Divine, Michael E., Pruneda, Jonathan N., Mostowy, Serge |
| المصدر: |
Journal of Cell Science; Apr2023, Vol. 136 Issue 7, p1-11, 11p |
| مصطلحات موضوعية: |
SEPTINS, SHIGELLA, AUTOPHAGY, UBIQUITIN, UBIQUITINATION, CONFOCAL microscopy |
| مستخلص: |
During host cell invasion, Shigella escapes to the cytosol and polymerizes actin for cell-to-cell spread. To restrict cell-to-cell spread, host cells employ cell-autonomous immune responses including antibacterial autophagy and septin cage entrapment. How septins interact with the autophagy process to target Shigella for destruction is poorly understood. Here, we employed a correlative light and cryo-soft X-ray tomography (cryo-SXT) pipeline to study Shigella septin cage entrapment in its near-native state. Quantitative cryo-SXT showed that Shigella fragments mitochondria and enabled visualization of X-ray-dense structures (~30 nm resolution) surrounding Shigella entrapped in septin cages. Using Airyscan confocal microscopy, we observed lysine 63 (K63)-linked ubiquitin chains decorating septin-cage-entrapped Shigella. Remarkably, septins and K63 chains are present in separate bacterial microdomains, indicating they are recruited separately during antibacterial autophagy. Cryo-SXT and live-cell imaging revealed an interaction between septins and LC3B-positive membranes during autophagy of Shigella. Together, these findings demonstrate how septin-caged Shigella are targeted for autophagy and provide fundamental insights into autophagy-cytoskeleton interactions. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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