دورية أكاديمية
Structural basis for cooperativity of human monoclonal antibodies to meningococcal factor H-binding protein
| العنوان: | Structural basis for cooperativity of human monoclonal antibodies to meningococcal factor H-binding protein |
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| المؤلفون: | Peschiera I, Giuliani M, Giusti F, Melero R, Paccagnini E, Donnarumma D, Pansegrau W, Carazo JM, Sorzano COS, Scarselli M, Masignani V, Liljeroos LJ, Ferlenghi I. |
| المساهمون: | Peschiera, I, Giuliani, M, Giusti, F, Melero, R, Paccagnini, E, Donnarumma, D, Pansegrau, W, Carazo, Jm, Sorzano, Co, Scarselli, M, Masignani, V, Liljeroos, Lj, Ferlenghi, I. |
| سنة النشر: | 2019 |
| المجموعة: | Università degli Studi di Messina: IRIS |
| مصطلحات موضوعية: | Cryoelectron microscopy, Cryoelectron tomography, Meningiti, Antibodies, Monoclonal, Antigens, Bacterial, Bacterial Protein, Blood Bactericidal Activity, Complement Factor H, Epitope Mapping, Human, Meningococcal Vaccine, Microscopy, Electron, Transmission, Surface Plasmon Resonance |
| الوصف: | Monoclonal antibody (mAb) cooperativity is a phenomenon triggered when mAbs couples promote increased bactericidal killing compared to individual partners. Cooperativity has been deeply investigated among mAbs elicited by factor H-binding protein (fHbp), a Neisseria meningitidis surface-exposed lipoprotein and one of the key antigens included in both serogroup B meningococcus vaccine Bexsero and Trumenba. Here we report the structural and functional characterization of two cooperative mAbs pairs isolated from Bexsero vaccines. The 3D electron microscopy structures of the human mAb-fHbp-mAb cooperative complexes indicate that the angle formed between the antigen binding fragments (fAbs) assume regular angle and that fHbp is able to bind simultaneously and stably the cooperative mAbs pairs and human factor H (fH) in vitro. These findings shed light on molecular basis of the antibody-based mechanism of protection driven by simultaneous recognition of the different epitopes of the fHbp and underline that cooperativity is crucial in vaccine efficacy. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/31263785; volume:2; issue:1; firstpage:241; lastpage:249; numberofpages:9; journal:COMMUNICATIONS BIOLOGY; https://hdl.handle.net/11570/3229743Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85070987465 |
| DOI: | 10.1038/s42003-019-0493-4 |
| الإتاحة: | https://doi.org/10.1038/s42003-019-0493-4Test https://hdl.handle.net/11570/3229743Test |
| رقم الانضمام: | edsbas.2FB20B41 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s42003-019-0493-4 |
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