التفاصيل البيبلوغرافية
| العنوان: |
Osteonectin RNA and collagen α1(I) RNA in the developing rat maxilla. |
| المؤلفون: |
Liao, Haihong1,2, Brandsten, Catharina1,2, Lundmark, Carin1, Christersson, Cecilia1, Wurtz, Tilmann1 |
| المصدر: |
European Journal of Oral Sciences. Jan1998 Supplement, Vol. 106, p418-423. 6p. |
| مصطلحات موضوعية: |
*OSTEONECTIN, *MESSENGER RNA, *COLLAGEN, *MAXILLA, *LABORATORY rats, *EXTRACELLULAR matrix, *ODONTOBLASTS, *FIBROBLASTS |
| مستخلص: |
The sequence of rat osteonectin mRNA was determined. Comparison with osteonectin (ON) mRNA sequences of other vertebrates showed a great similarity, but a stretch of codons deviated with respect to another rat strain, suggesting the possibility of two ON variants in rats. Northern blots exhibited one band of ON mRNA only. The expression of ON and collagen α1(I) RNA in tissues of the developing rat maxilla was studied by in situ hybridization. Both ON and collagen α1(I) RNA were observed concomitantly in osteoblasts, starting from the onset of bone formation at day 17 post coitum through the oldest age examined, day 20 after birth. A strict co-expression of the two sequences was also observed in odontoblasts as well as in fibroblasts of the periodontal ligament. In ameloblasts, neither ON nor collagen α1(I) RNA was detected under stringent hybridization conditions, but lower stringency led to an ON signal. Considering that ON is a secretory protein and the high stability of the ON mRNA, the co-expression of collagen α1(I) and ON RNA sequences in matrix-forming cells provided evidence that ON is a substantial component of collagen matrices. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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