دورية أكاديمية
In vivo imaging of reactive oxygen and nitrogen species in murine colitis
| العنوان: | In vivo imaging of reactive oxygen and nitrogen species in murine colitis |
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| المؤلفون: | Asghar, Muhammad Nadeem, Emani, R, Alam, Catharina, Helenius, Terhi, Grönroos, TJ, Sareila, O, U Din, Muezz, Holmdahl, R, Hänninen, A, Toivola, Diana |
| المصدر: | Asghar , M N , Emani , R , Alam , C , Helenius , T , Grönroos , TJ , Sareila , O , U Din , M , Holmdahl , R , Hänninen , A & Toivola , D 2014 , ' In vivo imaging of reactive oxygen and nitrogen species in murine colitis ' , Inflammatory Bowel Diseases , vol. 20 , no. 8 , pp. 1435–1447 . https://doi.org/10.1097/MIB.0000000000000118Test |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | in vivo imaging, colitis |
| الوصف: | BACKGROUND: Traditional techniques analyzing mouse colitis are invasive, laborious, or indirect. Development of in vivo imaging techniques for specific colitis processes would be useful for monitoring disease progression and/or treatment effectiveness. The aim was to evaluate the applicability of the chemiluminescent probe L-012, which detects reactive oxygen and nitrogen species, for in vivo colitis imaging. METHODS: Two genetic colitis mouse models were used; K8 knockout (K8(-/-)) mice, which develop early colitis and the nonobese diabetic mice, which develop a transient subclinical colitis. Dextran sulphate sodium was used as a chemical colitis model. Mice were anesthetized, injected intraperitoneally with L-012, imaged, and quantified for chemiluminescent signal in the abdominal region using an IVIS camera system. RESULTS: K8(-/-) and nonobese diabetic mice showed increased L-012-mediated chemiluminescence from the abdominal region compared with control mice. L-012 signals correlated with the colitis phenotype assessed by histology and myeloperoxidase staining. Although L-012 chemiluminescence enabled detection of dextran sulphate sodium-induced colitis at an earlier time point compared with traditional methods, large mouse-to-mouse variations were noted. In situ and ex vivo L-012 imaging as well as [18F]FDG-PET imaging of K8(-/-) mice confirmed that the in vivo signals originated from the distal colon. L-012 in vivo imaging showed a wide variation in reactive oxygen and nitrogen species in young mice, irrespective of K8 genotype. In aging mice L-012 signals were consistently higher in K8(-/-) as compared to K8(+/+) mice. CONCLUSIONS: In vivo imaging using L-012 is a useful, simple, and cost-effective tool to study the level and longitudinal progression of genetic and possibly chemical murine colitis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://research.abo.fi/en/publications/8f5ac8c0-dfe3-4c32-8496-a7abfdf241c2Test |
| DOI: | 10.1097/MIB.0000000000000118 |
| الإتاحة: | https://doi.org/10.1097/MIB.0000000000000118Test https://research.abo.fi/en/publications/8f5ac8c0-dfe3-4c32-8496-a7abfdf241c2Test |
| حقوق: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.C2D7857E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1097/MIB.0000000000000118 |
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