دورية أكاديمية
CD117 + dendritic and mast cells are dependent on RasGRP4 to function as accessory cells for optimal natural killer cell-mediated responses to lipopolysaccharide
| العنوان: | CD117 + dendritic and mast cells are dependent on RasGRP4 to function as accessory cells for optimal natural killer cell-mediated responses to lipopolysaccharide |
|---|---|
| المؤلفون: | Zhou, S, Tanaka, K, O'Keeffe, M, Qi, M, El-Assaad, F, Weaver, JC, Chen, G, Weatherall, C, Wang, Y, Giannakopoulos, B, Chen, L, Yu, DM, Hamilton, MJ, Wensing, LA, Stevens, RL, Krilis, SA |
| المساهمون: | Wong, G William |
| المصدر: | urn:ISSN:1932-6203 ; PLoS ONE, 11, 3, e0151638 |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2016 |
| المجموعة: | UNSW Sydney (The University of New South Wales): UNSWorks |
| مصطلحات موضوعية: | Emerging Infectious Diseases, Infectious Diseases, Biodefense, Vaccine Related, Prevention, 2 Aetiology, 2.1 Biological and endogenous factors, 1.1 Normal biological development and functioning, 1 Underpinning research, Inflammatory and immune system, Animals, Coculture Techniques, Dendritic Cells, Interferon-gamma, Killer Cells, Natural, Lipopolysaccharides, Mast Cells, Membrane Glycoproteins, Mice, Inbred C57BL, OX40 Ligand, Proto-Oncogene Proteins c-kit, Signal Transduction, Spleen, Tumor Necrosis Factors, ras Guanine Nucleotide Exchange Factors |
| الوصف: | Ras guanine nucleotide-releasing protein-4 (RasGRP4) is an evolutionarily conserved calcium-regulated, guanine nucleotide exchange factor and diacylglycerol/phorbol ester receptor. While an important intracellular signaling protein for CD117+ mast cells (MCs), its roles in other immune cells is less clear. In this study, we identified a subset of in vivo-differentiated splenic CD117+ dendritic cells (DCs) in wild-type (WT) C57BL/6 mice that unexpectedly contained RasGRP4 mRNA and protein. In regard to the biologic significance of these data to innate immunity, LPS-treated splenic CD117+ DCs from WT mice induced natural killer (NK) cells to produce much more interferon-γ (IFN-γ) than comparable DCs from RasGRP4-null mice. The ability of LPS-responsive MCs to cause NK cells to increase their expression of IFN-γ was also dependent on this intracellular signaling protein. The discovery that RasGRP4 is required for CD117+ MCs and DCs to optimally induce acute NK cell-dependent immune responses to LPS helps explain why this signaling protein has been conserved in evolution. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | http://hdl.handle.net/1959.4/unsworks_51816Test; https://unsworks.unsw.edu.au/bitstreams/55fba15e-e233-474e-8c29-175e21396cb8/downloadTest; https://doi.org/10.1371/journal.pone.0151638Test |
| DOI: | 10.1371/journal.pone.0151638 |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0151638Test http://hdl.handle.net/1959.4/unsworks_51816Test https://unsworks.unsw.edu.au/bitstreams/55fba15e-e233-474e-8c29-175e21396cb8/downloadTest |
| حقوق: | open access ; https://purl.org/coar/access_right/c_abf2Test ; CC BY ; https://creativecommons.org/licenses/by/4.0Test/ ; free_to_read |
| رقم الانضمام: | edsbas.48BF42C5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0151638 |
|---|