التفاصيل البيبلوغرافية
| العنوان: |
Sumoylation of the Plant Clock Transcription Factor CCA1 Suppresses DNA Binding. |
| المؤلفون: |
Hansen, Louise L.1, Imrie, Lisa1, Le Bihan, Thierry1, van den Burg, Harrold A.2, van Ooijen, Gerben1 gerben.vanooijen@ed.ac.uk |
| المصدر: |
Journal of Biological Rhythms. Dec2017, Vol. 32 Issue 6, p570-582. 13p. |
| مصطلحات موضوعية: |
*TRANSCRIPTION factors, *POST-translational modification, *DNA-protein interactions, *ARABIDOPSIS proteins, *CLOCK genes, *PROMOTERS (Genetics), *IMMUNOPRECIPITATION |
| مستخلص: |
In plants, the circadian clock regulates the expression of one-third of all transcripts and is crucial to virtually every aspect of metabolism and growth. We now establish sumoylation, a posttranslational protein modification, as a novel regulator of the key clock protein CCA1 in the model plant Arabidopsis. Dynamic sumoylation of CCA1 is observed in planta and confirmed in a heterologous expression system. To characterize how sumoylation might affect the activity of CCA1, we investigated the properties of CCA1 in a wild-type plant background in comparison with ots1 ots2, a mutant background showing increased overall levels of sumoylation. Neither the localization nor the stability of CCA1 was significantly affected. However, binding of CCA1 to a target promoter was significantly reduced in chromatin-immunoprecipitation experiments. In vitro experiments using recombinant protein revealed that reduced affinity to the cognate promoter element is a direct consequence of sumoylation of CCA1 that does not require any other factors. Combined, these results suggest sumoylation as a mechanism that tunes the DNA binding activity of the central plant clock transcription factor CCA1. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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