المؤلفون: Wang, Yian, Yan, Qijia, Mo, Yongzhen, Liu, Yuhang, Wang, Yumin, Zhang, Shanshan, Guo, Can, Wang, Fuyan, Li, Guiyuan, Zeng, Zhaoyang, Xiong, Wei

المصدر: Molecular Cancer; 2/28/2022, Vol. 21 Issue 1, p1-20, 20p

مصطلحات موضوعية: CIRCULAR RNA, NASOPHARYNX cancer, NEOPLASTIC cell transformation, POLYMERASE chain reaction, CANCER invasiveness

مستخلص: Background: Circular RNAs play an important role in tumor genesis and progression, but they have not been sufficiently studied in patients with nasopharyngeal carcinoma (NPC). Methods: The circular RNA, circCAMSAP1, was screened in NPC cells by RNA sequencing analysis. The expression of circCAMSAP1 in NPC tissues was examined by real-time quantitative polymerase chain reaction (RT-qPCR) and in situ hybridization. Wound-healing, transwell, MTT and flow cytometry assays, and nude mouse tumor models were used to explore the effect of circCAMSAP1 on proliferation and metastasis of NPC in vitro or in vivo. The downstream proteins regulated by circCAMSAP1 were screened using mass spectrometry. The interaction between circCAMSAP1 and the SERPINH1 mRNA was identified using the circular RNA immunoprecipitation method and the luciferase reporter assay. The interaction between SERPINH1 and transcription factor c-Myc was verified through Co-immunoprecipitation (Co-IP) and immunofluorescence. The effect of c-Myc on the generation of circCAMSAP1 was examined through RT-qPCR and chromatin immunoprecipitation. Finally, the splicing factors that promote the production of circCAMSAP1 were explored by RT-qPCR and RNA immunoprecipitation (RIP). Results: We found that circCAMSAP1 was highly expressed in NPC tissues and promoted NPC proliferation and metastasis. Additionally, circCAMSAP1 promoted SERPINH1 expression through improved SERPINH1 mRNA stability by binding to the 3′-untranslated region (3'UTR) of SERPINH1. Highly expressed SERPINH1 reduced the ubiquitination-degradation rate of c-Myc, causing increased tumorigenesis. Meanwhile, c-Myc, cooperating with splicing factor 10 (SRSF10), could also promote CAMSAP1 pre-mRNA transcription and back-splicing, forming a positive feedback of circCAMSAP1 production, resulting in the proliferation and metastasis of NPC. Conclusions: Our findings revealed that circCAMSAP1 promotes NPC proliferation and metastasis by binding to the 3'UTR of SERPINH1, suggesting that the positive feedback of circCAMSAP1-SERPINH1-c-Myc may serve as a prognostic biomarker or therapeutic target in patients with NPC. [ABSTRACT FROM AUTHOR]

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المؤلفون: Mo, Yongzhen, Wang, Yumin, Zhang, Shuai, Xiong, Fang, Yan, Qijia, Jiang, Xianjie, Deng, Xiangying, Wang, Yian, Fan, Chunmei, Tang, Le, Zhang, Shanshan, Gong, Zhaojian, Wang, Fuyan, Liao, Qianjin, Guo, Can, Li, Yong, Li, Xiaoling, Li, Guiyuan, Zeng, Zhaoyang, Xiong, Wei

المصدر: Molecular Cancer; 8/31/2021, Vol. 20 Issue 1, p1-21, 21p

مصطلحات موضوعية: CIRCULAR RNA, NASOPHARYNX cancer, METASTASIS, REVERSE transcriptase polymerase chain reaction, PROTEIN binding

مستخلص: Background: Circular RNAs (circRNAs) are widely expressed in human cells and are closely associated with cancer development. However, they have rarely been investigated in the context of nasopharyngeal carcinoma (NPC). Methods: We screened a new circRNA, circRNF13, in NPC cells using next-generation sequencing of mRNA. Reverse transcription polymerase chain reaction and RNA fluorescence in situ hybridization were used to detect circRNF13 expression in 12 non-tumor nasopharyngeal epithelial (NPE) tissues and 36 NPC samples. Cell proliferation was detected using MTT and flow cytometry assays, and colony formation capability was detected using colony formation assays. Cell migration and invasion were analyzed using wound-healing and Transwell assays, respectively. Cell glycolysis was analyzed using the Seahorse glycolytic stress test. Glucose transporter type 1 (GLUT1) ubiquitination and SUMOylation modifications were analyzed using co-immunoprecipitation and western blotting. CircRNF13 and Small Ubiquitin-like Modifier 2 (SUMO2) interactions were analyzed using RNA pull-down and luciferase reporter assays. Finally, to test whether circRNF13 inhibited NPC proliferation and metastasis in vivo, we used a xenograft nude mouse model generated by means of subcutaneous or tail vein injection. Results: We found that circRNF13 was stably expressed at low levels in NPC clinical tissues and NPC cells. In vitro and in vivo experiments showed that circRNF13 inhibited NPC proliferation and metastasis. Moreover, circRNF13 activated the SUMO2 protein by binding to the 3′- Untranslated Region (3′-UTR) of the SUMO2 gene and prolonging the half-life of SUMO2 mRNA. Upregulation of SUMO2 promotes GLUT1 degradation through SUMOylation and ubiquitination of GLUT1, which regulates the AMPK-mTOR pathway by inhibiting glycolysis, ultimately resulting in the proliferation and metastasis of NPC. Conclusions: Our results revealed that a novel circRNF13 plays an important role in the development of NPC through the circRNF13-SUMO2-GLUT1 axis. This study implies that circRNF13 mediates glycolysis in NPC by binding to SUMO2 and provides an important theoretical basis for further elucidating the pathogenesis of NPC and targeted therapy. [ABSTRACT FROM AUTHOR]

: Copyright of Molecular Cancer is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Li, Mohan^1,2,3 (AUTHOR), Wang, Yian^1,4 (AUTHOR), Wu, Pan² (AUTHOR), Zhang, Shanshan³ (AUTHOR), Gong, Zhaojian⁵ (AUTHOR), Liao, Qianjin¹ (AUTHOR), Guo, Can² (AUTHOR), Wang, Fuyan² (AUTHOR), Li, Yong² (AUTHOR), Zeng, Zhaoyang^1,2 (AUTHOR), Yan, Qijia^1,2,3 (AUTHOR) yanqijia77@126.com, Xiong, Wei^1,2 (AUTHOR) xiongwei@csu.edu.cn

المصدر: Cancer Letters. Jun2023, Vol. 563, pN.PAG-N.PAG. 1p.

مصطلحات موضوعية: *CANCER vaccines, *CIRCULAR RNA, *NON-coding RNA, *TUMOR proteins, *PEPTIDES, *SYNCRIP protein, *BACTERIAL vaccines

مستخلص: Neoantigen is a protein produced by mutant gene, which is only expressed in tumor cells. It is an ideal target for therapeutic tumor vaccines. Although synthetic long peptide (SLP)-based neoantigen vaccine, DNA-based neoantigen vaccine, and mRNA-based neoantigen vaccine are all in the development stage, they have some inherent shortcomings. Therefore, researchers turned their attention to a new type of "non-coding RNA (ncRNA)", circular RNA (circRNA), for potential better choice. Because of its unique high stability and protein-coding capacity, circRNA is a promising target in the field of neoantigen vaccine. In this paper, we reviewed the feasibility of circRNA encoding neoantigens, summarized the construction process, explained the mechanism of circRNA vaccine in vitro , and discussed the advantages and disadvantages of circRNA vaccine and possible combination with other immunotherapies. • Tumor vaccine is a new direction of cancer prevention and treatment. • Tumor neoantigens are tumor specific proteins and are ideal targets for tumor vaccines. • circRNA can be used as a vector for tumor vaccines because of its ability to encode small peptides. • In this paper, the construction method and application prospect of circRNA vaccine are described in detail. [ABSTRACT FROM AUTHOR]

المؤلفون: Qu, Hongke^1,2 (AUTHOR), Chen, Mingjian² (AUTHOR), Ge, Junshang² (AUTHOR), Zhang, Xiangyan³ (AUTHOR), He, Shuyi² (AUTHOR), Xiong, Fang⁴ (AUTHOR), Yan, Qijia⁴ (AUTHOR), Zhang, Shanshan⁴ (AUTHOR), Gong, Zhaojian⁵ (AUTHOR), Guo, Can^1,2 (AUTHOR), Wang, Fuyan^1,2 (AUTHOR), Zeng, Zhaoyang^1,2,6 (AUTHOR), Li, Xiaoling^1,2,6 (AUTHOR), Li, Guiyuan^1,2,6 (AUTHOR), Xiong, Wei^1,2,6 (AUTHOR) xiongwei@csu.edu.cn, Wu, Xu^1,2,6 (AUTHOR) wuxu1028@csu.edu.cn

المصدر: Analytica Chimica Acta. Jan2022, Vol. 1189, pN.PAG-N.PAG. 1p.

مصطلحات موضوعية: *CIRCULAR RNA, *EXONUCLEASES, *RNA, *FLUORESCENCE, *EPSTEIN-Barr virus, *CELL determination

مستخلص: Circular Ribonucleic Acid (CircRNA) plays regulatory roles in many biological processes, such as tumors and metabolic diseases. Due to the fact that circRNA is more stable and conservative than linear RNA, circRNA has become a potential biomarker in early clinical diagnosis and biomedical research. Therefore, the quantification of circRNA expression level is of importance for understanding their functions and their applications for disease diagnosis and treatment. Nevertheless, due to the low abundance of circRNA, it is still a challenge for the analysis of circRNA in cells. Herein, we proposed a sensitive detection method for circRNA based on the T7 exonuclease-assisted cycling enzymatic amplification. The fluorescent sensor was constructed by a hairpin molecular beacon and T7 exonuclease. With the cycling enzymatic amplification process, this sensor achieved the limit of detection of 1 pM with a good linear correlation in the range of 0–100 pM (R2 = 0.9891) using circBART2.2 as a model. Furthermore, we applied the proposed method in the determination of circBART2.2 in cell lysates. The results demonstrated that this method has promising applications in early diagnosis of Epstein-Barr virus (EBV) infection-related diseases using circRNA as the biomarker. [Display omitted] • The circRNA encoded by EBV was specifically detected by fluorescence sensor for the first time. • The enzyme cycle signal amplification strategy greatly improves the sensitivity of detection. • The applicability of this method in real samples was verified by a variety of cell lines. • The developed sensor can be expanded for other circRNA analysis in different diseases. [ABSTRACT FROM AUTHOR]