A molecular clone of Chronic Bee Paralysis Virus (CBPV) causes mortality in honey bee pupae (Apis mellifera)
| العنوان: | A molecular clone of Chronic Bee Paralysis Virus (CBPV) causes mortality in honey bee pupae (Apis mellifera) |
|---|---|
| المؤلفون: | Kerstin Seitz, Alžbeta Dikunová, Karen Power, Benjamin Lamp, Pavel Plevka, Till Rümenapf, Katharina Buczolich |
| المصدر: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, 030106 microbiology, lcsh:Medicine, Viral transmission, Insect Viruses, Chronic bee paralysis virus, Biology, Virus Replication, Genome, Article, Virus, Animal Diseases, Open Reading Frames, 03 medical and health sciences, law, law.legal_case, Animals, Cloning, Molecular, ORFS, lcsh:Science, Gene, Phylogeny, Multidisciplinary, lcsh:R, fungi, RNA virus, Sequence Analysis, DNA, Honey bee, Bees, biology.organism_classification, Virology, 3. Good health, 030104 developmental biology, Viral replication, behavior and behavior mechanisms, lcsh:Q, Viral pathogenesis |
| الوصف: | Among the many diseases compromising the well-being of the honey bee (Apis mellifera) the chronic paralysis syndrome of adult honey bees is one of the best described. The causative agent, chronic bee paralysis virus (CBPV), is a positive sense, single-stranded RNA virus with a segmented genome. Segment 1 encodes three putative open reading frames (ORFs), including the RNA-dependent RNA polymerase and other non-structural protein coding regions. Segment 2 encodes four putative ORFs, which contain the genes of supposed structural proteins. In this study, we established a reverse genetic system for CBPV by molecular cloning of DNA copies of both genome segments. CBPV rescue was studied in imago and honey bee pupae infection models. Virus replication and progeny virus production was only initiated when capped RNAs of both genome segments were injected in honey bees. As injection of these clonal RNAs caused clinical symptoms similar to wild-type CBPV infection, we conclude that the novel molecular clone fulfilled Koch’s postulates. Our virus clone will enable in-depth analysis of CBPV pathogenesis and help to increase knowledge about this important honey bee disease. |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac9f925b4323d9ef0305b3a091380529Test https://doi.org/10.1038/s41598-019-52822-1Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ac9f925b4323d9ef0305b3a091380529 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
|---|