التفاصيل البيبلوغرافية
| العنوان: |
β-Elemene Inhibits Cell Proliferation by Regulating the Expression and Activity of Topoisomerases I and IIα in Human Hepatocarcinoma HepG-2 Cells. |
| المؤلفون: |
Gong, Min, Liu, Ying, Zhang, Jian, Gao, Ya-jie, Zhai, Ping-ping, Su, Xi, Li, Xiang, Li, Yan, Hou, Li, Cui, Xiao-nan |
| المصدر: |
BioMed Research International; 6/29/2015, Vol. 2015, p1-10, 10p |
| مصطلحات موضوعية: |
DNA analysis, APOPTOSIS, BIOLOGICAL assay, CELL culture, CELL cycle, CELL lines, CELL physiology, ENZYMES, FLOW cytometry, HEPATOCELLULAR carcinoma, HYDROCARBONS, BOTANIC medicine, CHINESE medicine, MICROSCOPY, MOLECULAR structure, RESEARCH funding, STATISTICS, WESTERN immunoblotting, PLANT extracts, DATA analysis, DATA analysis software, DESCRIPTIVE statistics, ONE-way analysis of variance |
| مستخلص: |
Objective. To investigate the effects of β-Elemene (β-ELE) on the proliferation, apoptosis, and topoisomerase I (TOPO I) and topoisomerase IIα (TOPO IIα) expression and activity of human hepatocarcinoma HepG-2 cells. Methods. After treatment with β-ELE, morphological alterations of HepG-2 cells were observed under an inverted microscope. Cell proliferation was assessed using an MTT assay, cell cycles were analyzed using flow cytometry, and apoptosis was detected by Annexin V/PI staining. The expression of TOPO I and TOPO IIα was analyzed by Western blot techniques, and their activity was measured using the TOPO I-mediated, supercoiled pBR322 DNA relaxation and TOPO IIα-mediated Kinetoplast DNA (kDNA) decatenation assays, respectively. Supercoiled pBR322 and kDNA were also used to determine the direct effect of β-ELE on DNA breaks. Results. β-ELE significantly inhibited HepG-2 cell proliferation in a dose- and time-dependent manner. β-ELE also induced tumor cell arrest at S phase, induced cell apoptosis, and downregulated the protein expression of TOPO I and TOPO IIα in a dose-dependent manner. β-ELE also inhibited TOPO I- and TOPO IIα-mediated DNA relaxation but did not directly induce DNA breakage at any concentration. Conclusion. β-ELE could inhibit the proliferation of HepG-2 cells and interfere with the expression and activity of TOPO I and TOPO IIα. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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