Cellular processes of v-Src transformation revealed by gene profiling of primary cells - Implications for human cancer
| العنوان: | Cellular processes of v-Src transformation revealed by gene profiling of primary cells - Implications for human cancer |
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| المؤلفون: | Lizhen Wang, Bart M. Maślikowski, Natalie Rodrigues, Pierre-André Bédard, Germain Gillet, Ying Wu, Benjamin D. Néel |
| المصدر: | BMC Cancer, Vol 10, Iss 1, p 41 (2010) BMC Cancer |
| بيانات النشر: | BMC, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Cancer Research, Cell type, Lung Neoplasms, Breast Neoplasms, Chick Embryo, lcsh:RC254-282, Disease-Free Survival, Retina, Oncogene Protein pp60(v-src), Cohort Studies, 03 medical and health sciences, 0302 clinical medicine, Gene expression, Genetics, Animals, Cluster Analysis, Humans, Neoplasm Metastasis, Gene, 030304 developmental biology, Oligonucleotide Array Sequence Analysis, 0303 health sciences, Rous sarcoma virus, biology, Gene Expression Profiling, Fibroblasts, biology.organism_classification, Prognosis, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, 3. Good health, Cell biology, Gene expression profiling, Gene Expression Regulation, Neoplastic, Genes, src, Cell Transformation, Neoplastic, Oncology, 030220 oncology & carcinogenesis, v-Src, Cancer research, Tyrosine kinase, Proto-oncogene tyrosine-protein kinase Src, Research Article |
| الوصف: | BackgroundCell transformation by the Src tyrosine kinase is characterized by extensive changes in gene expression. In this study, we took advantage of several strains of the Rous sarcoma virus (RSV) to characterize the patterns of v-Src-dependent gene expression in two different primary cell types, namely chicken embryo fibroblasts (CEF) and chicken neuroretinal (CNR) cells. We identified a common set of v-Src regulated genes and assessed if their expression is associated with disease-free survival using several independent human tumor data sets.MethodsCEF and CNR cells were infected with transforming, non-transforming, and temperature sensitive mutants of RSV to identify the patterns of gene expression in response to v-Src-transformation. Microarray analysis was used to measure changes in gene expression and to define a common set of v-Src regulated genes (CSR genes) in CEF and CNR cells. A clustering enrichment regime using the CSR genes and two independent breast tumor data-sets was used to identify a 42-gene aggressive tumor gene signature. The aggressive gene signature was tested for its prognostic value by conducting survival analyses on six additional tumor data sets.ResultsThe analysis of CEF and CNR cells revealed that cell transformation by v-Src alters the expression of 6% of the protein coding genes of the genome. A common set of 175 v-Src regulated genes (CSR genes) was regulated in both CEF and CNR cells. Within the CSR gene set, a group of 42 v-Src inducible genes was associated with reduced disease- and metastasis-free survival in several independent patient cohorts with breast or lung cancer. Gene classes represented within this group include DNA replication, cell cycle, the DNA damage and stress responses, and blood vessel morphogenesis.ConclusionBy studying the v-Src-dependent changes in gene expression in two types of primary cells, we identified a set of 42 inducible genes associated with poor prognosis in breast and lung cancer. The identification of these genes provides a set of biomarkers of aggressive tumor behavior and a framework for the study of cancer cells characterized by elevated Src kinase activity. |
| اللغة: | English |
| تدمد: | 1471-2407 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4cb9edb324fb04c0e982b94d6825b35Test http://www.biomedcentral.com/1471-2407/10/41Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c4cb9edb324fb04c0e982b94d6825b35 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712407 |
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