Transcriptomic analysis of global changes in cytokine expression in mouse spleens following acute Toxoplasma gondii infection
| العنوان: | Transcriptomic analysis of global changes in cytokine expression in mouse spleens following acute Toxoplasma gondii infection |
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| المؤلفون: | Jun Ma, Xing-Quan Zhu, Jin-Lei Wang, Si-Yang Huang, Dong-Hui Zhou, Hui-Qun Song, Jun-Jun He |
| المصدر: | Parasitology Research. 115:703-712 |
| بيانات النشر: | Springer Science and Business Media LLC, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | 0301 basic medicine, Chemokine, Down-Regulation, Spleen, Real-Time Polymerase Chain Reaction, Transcriptome, Mice, Random Allocation, 03 medical and health sciences, Immune system, parasitic diseases, medicine, Animals, Humans, RNA, Messenger, Innate immune system, General Veterinary, biology, Sequence Analysis, RNA, Toxoplasma gondii, Chemotaxis, General Medicine, biology.organism_classification, Immunity, Innate, Up-Regulation, Cell biology, Toxoplasmosis, Animal, 030104 developmental biology, Infectious Diseases, medicine.anatomical_structure, Insect Science, Immunology, biology.protein, Cytokines, Female, Parasitology, Chemokines, Signal transduction, Toxoplasma, RNA, Protozoan, Signal Transduction |
| الوصف: | Toxoplasma gondii is a global pathogen that infects a wide range of animals and humans. During T. gondii infection, the spleen plays an important role in coordinating the adaptive and innate immune responses. However, there is little information regarding the changes in global gene expression within the spleen following T. gondii infection. To address this gap in knowledge, we examined the transcriptome of the mouse spleen following T. gondii infection. We observed differential expression of 2310 transcripts under these conditions. Analysis of KEGG and GO enrichment indicated that T. gondii alters multiple immune signaling cascades. Most of differentially expressed GO terms and pathways were downregulated, while immune-related GO terms and pathways were upregulated with response to T. gondii infection in mouse spleen. Most cytokines were upregulated in infected spleens, and all differentially expressed chemokines were upregulated which enhanced the immune cells chemotaxis to promote recruitment of immune cells, such as neutrophils, eosinophils, monocytes, dendritic cells, macrophages, NK cells, basophils, B cells, and T cells. Although IFN-γ-induced IDO (Ido1) was upregulated in the present study, it may not contribute a lot to the control of T. gondii because most differentially expressed genes involved in tryptophan metabolism pathway were downregulated. Innate immunity pathways, including cytosolic nucleic acid sensing pathway and C-type lectins-Syk-Card9 signaling pathways, were upregulated. We believe our study is the first comprehensive attempt to define the host transcriptional response to T. gondii infection in the mouse spleen. |
| تدمد: | 1432-1955 0932-0113 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::311e07c5cc5a7d1431d99a6d41ee4d6dTest https://doi.org/10.1007/s00436-015-4792-5Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....311e07c5cc5a7d1431d99a6d41ee4d6d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14321955 09320113 |
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