المؤلفون: Jillian Chan, Robert Carruthers, Claire A. Sheldon, Eduardo V. Navajas, Marinko V. Sarunic, Magdalena A. Wirth, Julian Lo, Haaris M. Khan

المصدر: Retina. 41:2172-2178

مصطلحات موضوعية: Adult, Male, Noninvasive imaging, Susac Syndrome, genetic structures, Retinal Artery Occlusion, Computed Tomography Angiography, chemistry.chemical_compound, Retinal Diseases, medicine, Humans, In patient, Fluorescein Angiography, Aged, business.industry, Microangiopathy, Retinal Vessels, Retinal, General Medicine, Optical coherence tomography angiography, Middle Aged, medicine.disease, Skeleton (computer programming), Ophthalmology, chemistry, Female, sense organs, business, Nuclear medicine, Tomography, Optical Coherence

الوصف: PURPOSE To determine whether optical coherence tomography angiography is of diagnostic utility for Susac syndrome (SuS) by quantifying microvascular retinal changes. METHODS We enrolled 18 eyes of 9 healthy controls and 18 eyes of 9 patients with chronic SuS (12 had previous branch retinal artery occlusions and 6 were clinically unaffected). Images of the fovea were taken using an optical coherence tomography angiography system. Analysis included vessel density, fractal dimension, vessel diameter, and measurements of the foveal avascular zone (area, eccentricity, acircularity index, and axis ratio) in deep and superficial retinal layers. RESULTS Skeleton density and inner ring vessel density were significantly lower in patients with SuS (skeleton density: Susac 0.11 ± 0.01 vs. controls 0.12 ± 0.01, P = 0.027. VD: SuS 0.39 ± 0.04 vs. controls 0.42 ± 0.02, P = 0.041). Eccentricity and axis ratio were significantly higher in patients with SuS (EC: Susac 0.61 ± 0.11, controls 0.51 ± 0.10, P = 0.003; axis ratio: Susac 1.57 ± 0.28, controls 1.39 ± 0.11, P = 0.005). SuS eyes (affected and unaffected) had poorer outcomes of the remaining vascular parameters compared with controls (P > 0.05). CONCLUSION Optical coherence tomography angiography identified chronic microvascular changes in the eyes of patients with chronic SuS. Even clinically unaffected SuS eyes showed poorer vascular parameters. Although further research is needed, this noninvasive imaging modality seems to have the potential to serve as a valuable additive diagnostic tool.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8d2353d9512dce9bb1857acfc1172f3Test
https://doi.org/10.1097/iae.0000000000003170Test

المؤلفون: Leeann D. Louis, Shoshana Yakar, Mitchell B. Schaffler, Godze Yildirim, Manisha Dixit, John J. Kopchick, Andrzej Bartke, Silvana Duran-Ortiz, Sher Bahadur Poudel

المصدر: Aging Cell

مصطلحات موضوعية: Male, Senescence, Aging, medicine.medical_specialty, Medullary cavity, Matrix (biology), Biology, Spectrum Analysis, Raman, bone, Raman microspectroscopy, Mice, chemistry.chemical_compound, Internal medicine, medicine, Animals, Original Paper, micro‐CT, Cell Biology, Original Papers, Skeleton (computer programming), Sexual dimorphism, Bone Diseases, Metabolic, Endocrinology, medicine.anatomical_structure, chemistry, sexual dimorphism, Growth Hormone, Osteocyte, Body Composition, Sclerostin, Female, Bone marrow

الوصف: Somatopause refers to the gradual declines in growth hormone (GH) and insulin‐like growth factor‐1 throughout aging. To define how induced somatopause affects skeletal integrity, we used an inducible GH receptor knockout (iGHRKO) mouse model. Somatopause, induced globally at 6 months of age, resulted in significantly more slender bones in both male and female iGHRKO mice. In males, induced somatopause was associated with progressive expansion of the marrow cavity leading to significant thinning of the cortices, which compromised bone strength. We report progressive declines in osteocyte lacunar number, and increases in lacunar volume, in iGHRKO males, and reductions in lacunar number accompanied by ~20% loss of overall canalicular connectivity in iGHRKO females by 30 months of age. Induced somatopause did not affect mineral/matrix ratio assessed by Raman microspectroscopy. We found significant increases in bone marrow adiposity and high levels of sclerostin, a negative regulator of bone formation in iGHRKO mice. Surprisingly, however, despite compromised bone morphology, osteocyte senescence was reduced in the iGHRKO mice. In this study, we avoided the confounded effects of constitutive deficiency in the GH/IGF‐1 axis on the skeleton during growth, and specifically dissected its effects on the aging skeleton. We show here, for the first time, that induced somatopause compromises bone morphology and the bone marrow environment.
Induced somatopause during aging causes a drop in GH/IGF‐1 signaling, inhibits radial bone expansion and associates with increased bone marrow adiposity. At the bone tissue level, induced somatopause leads to progressive declines in osteocyte lacunar density and connectivity.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b25a3d93ce66a007e37b4ed8f8c8554aTest
https://doi.org/10.1111/acel.13505Test

المؤلفون: Dariusz Chlubek, Tomasz Olszowski, Irena Baranowska-Bosiacka, Maciej Sikora, Ewa Rębacz-Maron

المصدر: Journal of Trace Elements in Medicine and Biology. 51:115-122

مصطلحات موضوعية: Adult, Male, Facial bone, Adolescent, Alcohol Drinking, Alcohol, 010501 environmental sciences, 01 natural sciences, Biochemistry, Facial Bones, Inorganic Chemistry, Young Adult, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Bone Density, Residence Characteristics, medicine, Humans, Aged, 0105 earth and related environmental sciences, Aged, 80 and over, business.industry, Smoking, Middle Aged, Skeleton (computer programming), Human skeleton, medicine.anatomical_structure, chemistry, Molecular Medicine, Facial skeleton, Bone mineral content, Female, Residence, business, Alcohol consumption, 030217 neurology & neurosurgery, Demography

الوصف: Background: Environmental factors exert their influence on the living organism throughout ontogeny. More and more often, researchers find correlations between specific environmental factors and the so-called diseases of affluence. Deficits and excess of essential elements also leave their mark on the skeleton. Aim: To investigate the influence of alcohol consumption, tobacco smoking and place of residence, according to sex and calendar age, on the concentrations of micro-, macro- and toxic elements in human facial bones. Material & methods: Patients undergoing surgical treatment were examined for the mineral content in the collected bone material. The bone contents of the following elements were determined: Ca, K, Mg, Na, P, Fe, Zn, Mo, Ba, Mn, Li, Be, Co, B, Sr, Cr, Pb, Cd, Ni, and Al, depending on the type of facial bone, sex, calendar age, alcohol consumption, tobacco smoking and place of residence. Results: Sex and alcohol consumption showed the highest degree of correlation with the content of the minerals included in the study. Alcohol drinking was found to exert the strongest influence on women’s bodies, the highest number of statistically significant correlations was demonstrated between the content of minerals in the examined bones and alcohol drinking in women. Other factors included in the analysis had a different impact on men and women, the concentrations of elements included in the study differed depending on age, tobacco smoking and place of residence. Conclusions: The observed differences in the element mineral composition of the human facial skeleton may be explained by developmental specifics and functional adaptation. However, general biological characteristics (sex, age), environmental factors (place of residence), as well as smoking and alcohol use may exert significant influence on the concentrations of micro-, macro- and toxic elements in particular regions of the human skeleton. The impact of environmental factors is a very complex phenomenon, which may be stronger or more subtle, leaving its mark on the bone structure. The environmental factors included in the analysis had a different influence on men than women.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03b161966f8ebc92ba244b2fca6d4119Test
https://doi.org/10.1016/j.jtemb.2018.10.012Test

المؤلفون: Ewa Sadanowicz, Joanna Kwiatkowska, Agnieszka Matuszewska, Adam Szeląg, Marek Bolanowski, Marcin Landwójtowicz, Małgorzata Pieśniewska, Diana Jędrzejuk, Tomasz Sozański, Beata Nowak

المصدر: Pharmacological Reports. 70:951-954

مصطلحات موضوعية: Male, 0301 basic medicine, Peak bone mass, medicine.medical_specialty, Time Factors, Osteocalcin, Ranitidine, Bone tissue, Collagen Type I, Bone resorption, Bone remodeling, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Histamine H2 receptor, Bone Density, Internal medicine, medicine, Animals, Pharmacology, business.industry, Phosphorus, 030206 dentistry, General Medicine, Skeleton (computer programming), Peptide Fragments, Rats, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Histamine H2 Antagonists, chemistry, business, Histamine, medicine.drug

الوصف: Background Histamine regulates function of osteoclasts and osteoblasts, however data regarding the influence of histamine H2 receptors antagonists on bone tissue are ambiguous. Factors that influence growing skeleton may have an important impact on the peak bone mass and future risk of fractures. The aim of our study was the assessment of influence of ranitidine, on growing bones. Methods The experiment was carried out on young male Wistar rats divided into two groups receiving either ranitidine (10 mg/kg ip) or vehicle. Results A significant decrease in femoral BMD in ranitidine-treated rats (R) compared to vehicle-treated ones (C) was detected (0.262 ± 0.009 g/cm2 vs. 0.271 ±0.007 g/cm2, p Conclusions Long-term administration of ranitidine increases bone resorption and decreases bone formation in growing rats leading to decrease in BMD.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8e079c5f1967824b760c0f4db90c5c5Test
https://doi.org/10.1016/j.pharep.2018.03.005Test

المؤلفون: Maria Paula M. Marques, Maria Teresa Ferreira, Luís A. E. Batista de Carvalho, Francisco Curate, Mariana Pedrosa

المصدر: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Morphology (linguistics), Carbonates, Human bone, Pathology and Forensic Medicine, Phosphates, Chemometrics, chemistry.chemical_compound, Biological profile, Internal medicine, Age Determination by Skeleton, Spectroscopy, Fourier Transform Infrared, medicine, Humans, Femur, Aged, Aged, 80 and over, Chemistry, Bioapatite, Chemical anthropology, Forensic anthropology, IR spectroscopy, Humerus, Middle Aged, Skeleton (computer programming), Body Remains, Endocrinology, Carbonate, Forensic Anthropology, Female, Collagen

الوصف: In forensic anthropology, the application of traditional methods for estimating the biological profile of human skeletal remains is often hampered by poor preservation and skeletal representativeness, compromising their reliability. Thus, the development of alternative methods to the morphometric analysis of bones to estimate the biological profile of human remains is paramount. The age of an individual can cause changes in bone morphology, mass and size, as well as in its chemical composition. In this sense, the main objective of this research was to evaluate if the contents of bone collagen (Am/P), carbonate type A (API), carbonate type B (BPI), the relation between the carbonate content (types A and B) to type B carbonate (C/C), carbonate-phosphate ratio (C/P) and crystallinity index (CI), spectroscopic indices obtained from relationships between infrared absorption band intensities (FTIR-ATR), can be used as age-at-death predictors. A sample of femora and humeri from the 21st Century Identified Skeleton Collection (N = 80, 44 females and 36 males) was employed. Results show that, with advancing age, women’s femora have lower CI values, but BPI and C/P indices increase, and the deformation and disorder of the crystal lattice are probably affected by the integration of type B carbonate content of the femur. The ratios analysed, especially the CI and the BPI, show potential to estimate age-at-death in human skeletal remains, when sex is already known, thus helping to assess the biological profile when conventional methods cannot be applied.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26283977ff559b74abb172c831d21c94Test
https://pubmed.ncbi.nlm.nih.gov/32385593Test

المؤلفون: Tjebo F. C. Heeren, Philipp Herrmann, Peter Charbel Issa, Maximilian W. M. Wintergerst, Simone Tzaridis, Clarissa Mai, Frank G. Holz

المصدر: Investigative ophthalmologyvisual science. 60(10)

مصطلحات موضوعية: Male, medicine.medical_specialty, genetic structures, Multimodal Imaging, chemistry.chemical_compound, Ophthalmology, Medicine, Humans, Fluorescein Angiography, Macular telangiectasia, Aged, Plexus, medicine.diagnostic_test, business.industry, Choroid, Parafovea, Retinal Vessels, Retinal, Blood flow, Middle Aged, medicine.disease, Fluorescein angiography, Skeleton (computer programming), eye diseases, Cross-Sectional Studies, chemistry, Microvessels, Disease Progression, Retinal Telangiectasis, Female, sense organs, business, Perfusion, Tomography, Optical Coherence

الوصف: Purpose To quantify the retinal and choriocapillaris perfusion in different disease stages of macular telangiectasia type 2 (MacTel) using optical coherence tomography-angiography (OCT-A). Methods We examined 76 eyes of 76 patients and 24 eyes of 24 age-related controls. Participants underwent multimodal imaging, including OCT and OCT-A. Patients' eyes were divided into three groups considering predefined criteria from funduscopy, OCT, and fluorescein angiography, thus reflecting the disease severity ("early," "advanced," and "neovascular"). Quantitative analyses of vessel density (VD), skeleton density (SD), and fractal dimension (FD) were conducted in the superficial and deep retinal plexus and in the avascular layer. The choriocapillaris was analyzed for mean signal intensity and percentage of nondetectable perfused choriocapillaris-area (PNPA). Results The deep retinal plexus showed a progressive decrease of mean VD, SD, and FD in the temporal parafovea in all disease stages. In the superficial layer, VD, SD, and FD were significantly decreased in the temporal parafovea of advanced and neovascular stages, while these parameters did not differ from controls in early stages. In MacTel, signals of blood flow were also detectable at the level of the avascular layer and showed a significant increase with disease progression. The choriocapillaris in MacTel showed a significant increase of mean PNPA and a decrease of mean signal intensity in comparison to controls. These findings were consistent in all disease stages. Conclusions Quantitative OCT-A data show a progressive rarefication of the retinal microvasculature in MacTel. We propose an altered choriocapillaris perfusion as a possibly early alteration of the disease.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9d72f65f875df4375620cf191c5678dTest
https://pubmed.ncbi.nlm.nih.gov/31415079Test

المؤلفون: Rebecca K. Sawyer, Gerald J. Atkins, René St-Arnaud, Paul H. Anderson, Kate R. Barratt

المساهمون: Barratt, Kate R, Sawyer, Rebecca K, Atkins, Gerald J, St-Arnaud, Rene, Anderson, Paul H

المصدر: Bone. 143:115767

مصطلحات موضوعية: Male, 0301 basic medicine, Fibroblast growth factor 23, Vitamin, medicine.medical_specialty, Histology, Physiology, Endocrinology, Diabetes and Metabolism, vitamin D, 030209 endocrinology & metabolism, Rickets, XLH, Phosphates, fibroblast growth factor 23, Mice, 03 medical and health sciences, chemistry.chemical_compound, Hyp, 0302 clinical medicine, Internal medicine, rickets, medicine, Vitamin D and neurology, Animals, Humans, Vitamin D, Fibroblast, Chemistry, medicine.disease, Phosphate, Skeleton (computer programming), Diet, Fibroblast Growth Factor-23, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Dietary Supplements, Dihydroxycholecalciferols, Familial Hypophosphatemic Rickets, Hypophosphatemia

الوصف: The disorder of X-linked hypophosphatemia (XLH), results in the supressed renal production of active 1α,25-dihydroxyvitamin D (1,25(OH)2D) due to elevated fibroblast growth factor-23 (FGF23) levels. While adequate 25(OH)D levels are generally associated with improved mineralisation of the skeleton independent of circulating 1,25(OH)2D levels, it is unclear whether raising 25(OH)D to sufficiently high levels through dietary vitamin D3 administration contributes to improving bone mineralisation in the murine homolog for XLH, Hyp mice. Three-week-old male Hyp mice were fed one of four diets containing either 1000 IU (C) or 20,000 IU (D) vitamin D3/kg diet with either 0.35% phosphate or 1.25% phosphate (P) until 12 weeks of age (n = 12/group). When compared to C-fed mice, D-fed mice significantly elevated serum 25(OH)D levels to 72.8 ± 4.9 nmol/L (2-fold, p < 0.001) and increased both cortical bone mineral density (15%, p < 0.01), and vertebral trabecular BV/TV% (80%, p < 0.001), despite persistent hypophosphatemia and normocalcemia. The increase in bone volume was associated with improved Tb.Th (12%, p < 0.01) and Tb.N (63%, p < 0.001). Unlike with D-diet, P-fed mice resulted in increased femoral (15%, p < 0.001) and vertebral (12%, p < 0.001) length, and a 34% increase in vertebral trabecular BV/TV% when compared to control fed animals (p < 0.001). However, the addition of the high P diet to the high D diet did not result in additive effects on bone mineralisation when compared to the effects of D diet alone, despite serum 25(OH)D levels elevated to 118.8 ± 8.6 nmol/L. In D-fed mice, the increase in bone mineral density and volume was associated with reduced osteoid volume, reduced ObS/BS, and a trend for reduced serum PTH levels, suggesting reduced bone turnover in these animals. Thus, elevating serum 25(OH)D levels independently improves bone mineralisation in Hyp mice without causing hypercalcemia, suggesting that further studies are required in XLH patients to establish the role of increasing 25(OH)D levels in improving bone mineralisation. Refereed/Peer-reviewed

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4a1af97987bb318049a939bbf00a10aTest
https://doi.org/10.1016/j.bone.2020.115767Test

المؤلفون: Basma Khoury, Benjamin P. Sinder, Michelle S. Caird, Kai Cortright, Terese N. Jenks, Bethany Meyer, Kenneth M. Kozloff, Ferrous S. Ward, Joseph E. Perosky, David K. Barton, Joan C. Marini

المصدر: Bone. 93:79-85

مصطلحات موضوعية: Male, 0301 basic medicine, medicine.medical_specialty, Histology, Anabolism, Physiology, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Osteoporosis, Osteoclasts, 030209 endocrinology & metabolism, Antibodies, Bone and Bones, Article, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Internal medicine, Cortical Bone, medicine, Cathepsin K, Animals, Femur, Bone Resorption, Adaptor Proteins, Signal Transducing, Glycoproteins, Spectroscopy, Near-Infrared, Diphosphonates, Dose-Response Relationship, Drug, business.industry, Organ Size, X-Ray Microtomography, Osteogenesis Imperfecta, Bisphosphonate, medicine.disease, Skeleton (computer programming), Biomechanical Phenomena, Disease Models, Animal, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, chemistry, Osteogenesis imperfecta, Intercellular Signaling Peptides and Proteins, Sclerostin, Cortical bone, business

الوصف: Sclerostin antibody has demonstrated a bone-forming effect in pre-clinical models of osteogenesis imperfecta, where mutations in collagen or collagen-associated proteins often result in high bone fragility in pediatric patients. Cessation studies in osteoporotic patients have demonstrated that sclerostin antibody, like intermittent PTH treatment, requires sequential anti-resorptive therapy to preserve the anabolic effects in adult populations. However, the persistence of anabolic gains from either drug has not been explored clinically in OI, or in any animal model. To determine whether cessation of sclerostin antibody therapy in a growing OI skeleton requires sequential anti-resorptive treatment to preserve anabolic gains in bone mass, we treated 3 week old Brtl/+ and wild type mice for 5 weeks with SclAb, and then withdrew treatment for an additional 6 weeks. Trabecular bone loss was evident following cessation, but was preserved in a dose-dependent manner with single administration of pamidronate at the time of cessation. In vivo longitudinal near-infrared optical imaging of cathepsin K activation in the proximal tibia suggests an anti-resorptive effect of both SclAb and pamidronate which is reversed after three weeks of cessation. Cortical bone was considerably less susceptible to cessation effects, and showed no structural or functional deficits in the absence of pamidronate during this cessation period. In conclusion, while SclAb induces a considerable anabolic gain in the rapidly growing Brtl/+ murine model of OI, a single sequential dose of antiresorptive drug is required to maintain bone mass at trabecular sites for 6 weeks following cessation.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::388d667d8c19eff68366428ad00d19a6Test
https://doi.org/10.1016/j.bone.2016.09.013Test

المؤلفون: Amy Z. Mundorff, Alexandra L. Emmons, Jennifer M. DeBruyn, Jonathan Davoren

المصدر: Forensic science international. Genetics. 44

مصطلحات موضوعية: 0301 basic medicine, DNA, Bacterial, Male, Burial, Biology, Bone and Bones, Pathology and Forensic Medicine, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Genetics, Humans, 030216 legal & forensic medicine, DNA, Fungal, Cuneiform, Disinterment, Dna concentration, Fungal DNA, DNA, Intra individual, Skeleton (computer programming), DNA Fingerprinting, Body Remains, 030104 developmental biology, chemistry, Evolutionary biology, Postmortem Changes, Cancellous Bone, Microsatellite, Female, Microsatellite Repeats

الوصف: Our ability to identify skeletal remains often relies on the quality and quantity of DNA extracted from bone and teeth. Current research on buried remains has been retrospective, and no study to our knowledge has comprehensively assessed both intra-individual and inter-individual variation in human skeletal DNA from all representative skeletal element types recovered from a burial. Three individuals were interred together in a single grave for four years. Following disinterment, skeletal DNA was extracted, quantified, and GlobalFiler™ results were produced from 49 bones per skeleton, representing all bone types. Multiple sites per bone were also tested to determine intra-bone variability. Co-extracted bacterial and fungal DNA were quantified to determine microbial loads in the bones. Results show that the small, cancellous bones of the feet outperformed other bones in terms of DNA yield, measured as nanograms per gram of bone powder, and short tandem repeat (STR) profile completeness. The cuneiforms, in particular, had consistently high human DNA yields for all three individuals. DNA yield varied by individual and depth within the grave, with the shallowest individual demonstrating the highest DNA yields While the feet exhibited the greatest variation in DNA yield across bone type and sampling site, they also demonstrated some of the highest DNA yields and the most complete STR profiles, evoking a re-evaluation of their use for skeletal DNA sampling and analysis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87ee531d525701dbb9cb2face20f8dd9Test
https://pubmed.ncbi.nlm.nih.gov/31710897Test

المؤلفون: Liang Ming Zhang, Chunyi Wen, Chun Xiao Luo, Bin Liu, Limin Rong, Zhongyu Liu, Man Ting Au, Tian Wei He, Bu Yang

المصدر: BioMed Research International
BioMed Research International, Vol 2019 (2019)

مصطلحات موضوعية: 0301 basic medicine, Male, medicine.medical_specialty, Article Subject, medicine.medical_treatment, Intraperitoneal injection, Urology, lcsh:Medicine, 030209 endocrinology & metabolism, Pilot Projects, General Biochemistry, Genetics and Molecular Biology, Bone remodeling, 03 medical and health sciences, chemistry.chemical_compound, Mice, 0302 clinical medicine, Bone Density, medicine, Animals, Receptor, Vertebral bone, Macitentan, Mice, Inbred BALB C, Sulfonamides, Osteoblasts, General Immunology and Microbiology, Endothelin-1, business.industry, lcsh:R, Antagonist, General Medicine, Skeleton (computer programming), Spine, 030104 developmental biology, Pyrimidines, chemistry, Cancellous Bone, Hypertension, Immunohistochemistry, business, Research Article

الوصف: Purpose. Blood vessels and skeleton interact together. Endothelin-1 is a potent vasoconstrictor and also has an effect on bone metabolism. The dual antagonist to both endothelin-1 type A and B receptors, Macitentan, has been approved for clinical management of pulmonary arterial hypertension while little is known about the secondary effect of the drug on spine. We aimed to answer how vertebral bone mass responded to Macitentan treatment in mice. Methods. Sixteen male balb/c mice at 6 months were randomly assigned into 2 groups. Vehicle and Macitentan were administrated via intraperitoneal injection to Control group and Treatment group, respectively, for 4 months. At sacrifice, plasma endothelin-1 was evaluated with ELISA and vertebral bone mass was evaluated with Microcomputed Tomography and histological analysis. Results. We found higher plasma endothelin-1 level (p Conclusions. Treatment with Macitentan is associated with significant lower vertebral bone mass and therefore the secondary effect of dual antagonists to endothelin-1 receptors on the skeleton should be monitored and investigated in clinical practice. Both osteoblasts and osteoclasts may be involved while the molecular mechanism needs to be further explored.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db35a79baff14bc9716746cd53827edeTest
http://europepmc.org/articles/PMC6399551Test