Molecular characterization and expression studiesof Eucalyptus globulus stress-responsive gene DHN-10
| العنوان: | Molecular characterization and expression studiesof Eucalyptus globulus stress-responsive gene DHN-10 |
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| المؤلفون: | Qurban Ali, Z. Qamar, G.Z. Jahangir, M.Z. Saleem, S. Naz, A. Younas, M.I. Khan |
| المصدر: | Biologia plantarum. 65:316-322 |
| بيانات النشر: | Institute of Experimental Botany, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Expression vector, Accession number (library science), In silico, Plant Science, Horticulture, medicine.disease_cause, Amino acid, Biochemistry, chemistry, medicine, Coding region, Escherichia coli, Gene, Histidine |
| الوصف: | The Eucalyptus globulus is one of the important forest tree species which reveals enhanced tolerance to multiple abiotic stresses. The enhanced tolerance of E. globulus against low temperature is linked to the presence of enhanced accumulation of lysine-rich dehydrin proteins. The accumulation of dehydrin protein of Mr 10 kDa in response to multiple abiotic stresses prevents cells from dehydration. It is encoded by a novel dehydrin-10 (DHN-10) gene. In the present study, we have used the DHN-10 gene of E. globulus of Pakistani origin, which was cloned in a bacterial expression vector pET30(a) and sequenced. We have found the 315 bp long coding sequence of this gene which has been enrolled as Eucalyptus GZJ-2018 DHN-10 gene under GenBank accession number MG948256.1. The in silico studies have identified several differences of this gene from the earlier enrolled DHN-10 genes: the studied gene possessed higher amphipathic character because of the presence of five additional electrically charged amino acids (two positively charged histidine, one negatively charged glutamate, and two negatively charged aspartate residues) and one extra lysine residue. The studied DHN-10 gene has been successfully expressed in the BL21-DE3 expression strain of Escherichia coli and 10 kDa protein has been detected on the nitrocellulose membrane. Our study is the first report of the sub-cloning of the DHN-10 gene and its expression outside the Eucalyptus cell. |
| تدمد: | 1573-8264 0006-3134 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::3bd86482cad601246d51cb6f7c61aa36Test https://doi.org/10.32615/bp.2019.107Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........3bd86482cad601246d51cb6f7c61aa36 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15738264 00063134 |
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