نتائج البحث

1

Retinoic acid can improve autophagy through depression of the PI3K-Akt-mTOR signaling pathway via RARα to restore spermatogenesis in cryptorchid infertile rats

المؤلفون: Dong Hu, Xing Liu, Jing Zhu, Guanghui Wei, Chunlan Long, Yihang Yu, Lianju Shen, Yu Zhou, Tao Xu, Deying Zhang, Tao Lin, Dawei He

المصدر: Genes & Diseases. 9:1368-1377

الوصف: Cryptorchidism-caused adult infertility is a common component of idiopathic reasons for male infertility. Retinoic acid (RA) has a vital effect on the spermatogenesis process. Here, we found that the expression of c-Kit, Stra8, and Sycp3 could be up-regulated via the activation of retinoic acid receptor α (RARα) after RA supplementation in neonatal cryptorchid infertile rats. We also demonstrated that the protein expression of PI3K, p-Akt/pan-Akt, and p-mTOR/mTOR was higher in cryptorchid than in normal testes, and could be suppressed with RA in vivo. After RA treatment in infertile cryptorchid testis in vivo, the levels of the autophagy proteins LC3 and Beclin1 increased and those of P62 decreased. Biotin tracer indicated that the permeability of blood-testis barrier (BTB) in cryptorchid rats decreased after RA administration. Additionally, after blocking the RARα with AR7 (an RARα antagonist) in testicle culture in vitro, we observed that compared with normal testes, the PI3K-Akt-mTOR signaling pathway and the autophagy pathway was increased and decreased, respectively, which were coincident with cryptorchisd testes in vivo. Additionally, the appropriate concentrations of RA treatment could depress the PI3K-Akt-mTOR signaling pathway and improve the autophagy pathway. The results confirmed that RA can rehabilitate BTB function and drive key protein levels in spermatogonial differentiation through depressing the PI3K-Akt-mTOR signaling pathway via RARα.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::156fd6bc5c7196736507da792ac06762Test
https://doi.org/10.1016/j.gendis.2021.03.006Test

2

دورية أكاديمية

Hibernation and Radioprotection: Gene Expression in the Liver and Testicle of Rats Irradiated under Synthetic Torpor

المؤلفون: Walter Tinganelli, Timna Hitrec, Fabrizio Romani, Palma Simoniello, Fabio Squarcio, Agnese Stanzani, Emiliana Piscitiello, Valentina Marchesano, Marco Luppi, Maximiliano Sioli, Alexander Helm, Gaetano Compagnone, Alessio G. Morganti, Roberto Amici, Matteo Negrini, Antonio Zoccoli, Marco Durante, Matteo Cerri

المصدر: International Journal of Molecular Sciences, Vol 20, Iss 2, p 352 (2019)

مصطلحات موضوعية: radiation, liver, testicle, synthetic torpor, torpor, hypothermia, hibernation, raphe pallidus, ATM, space exploration, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: Hibernation has been proposed as a tool for human space travel. In recent years, a procedure to induce a metabolic state known as “synthetic torpor” in non-hibernating mammals was successfully developed. Synthetic torpor may not only be an efficient method to spare resources and reduce psychological problems in long-term exploratory-class missions, but may also represent a countermeasure against cosmic rays. Here we show the preliminary results from an experiment in rats exposed to ionizing radiation in normothermic conditions or synthetic torpor. Animals were irradiated with 3 Gy X-rays and organs were collected 4 h after exposure. Histological analysis of liver and testicle showed a reduced toxicity in animals irradiated in torpor compared to controls irradiated at normal temperature and metabolic activity. The expression of ataxia telangiectasia mutated (ATM) in the liver was significantly downregulated in the group of animal in synthetic torpor. In the testicle, more genes involved in the DNA damage signaling were downregulated during synthetic torpor. These data show for the first time that synthetic torpor is a radioprotector in non-hibernators, similarly to natural torpor in hibernating animals. Synthetic torpor can be an effective strategy to protect humans during long term space exploration of the solar system.

العلاقة: http://www.mdpi.com/1422-0067/20/2/352Test; https://doaj.org/toc/1422-0067Test; https://doaj.org/article/1e55906bd6c94c79bd47062149721434Test

الإتاحة: https://doi.org/10.3390/ijms20020352Test
https://doaj.org/article/1e55906bd6c94c79bd47062149721434Test

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النص الكامل

3

Phycobiliproteins extract from Spirulina protects against single-dose cadmium-induced reproductive toxicity in male mice

المؤلفون: Germán Chamorro-Cevallos, José Melesio Cristóbal-Luna, Ricardo Iván Montaño-González, Jorge Briseño-Bugarín, María Angélica Mojica-Villegas, Gabriela Gutiérrez-Salmeán

المصدر: Environmental Science and Pollution Research. 29:17441-17455

الوصف: Cadmium (Cd) is known for its many toxic effects on male population such as hypogonadism and fertility difficulties, which are oftenly associated with oxidative stress. As beneficial food, Spirulina(Sp) has been proved efficient against the heavy metal toxicity. This capacity can be associated with its phycobiliproteins (PBP). In this study, the capability of PBP and Sp to treat Cd-induced oxidative damage on the testes and spermatozoa was considered. CD-1 strain mice were orally treated with either Sp or PBP for 10 days prior to single-dose Cd challenge. Sperm quality determinations and testicle histology analysis were performed. Testosterone on serum was measured using enzyme-linked immunosorbent assay (ELISA). Oxidative damage was determined. Antioxidant enzyme activity was analyzed by measuring the activity of super oxide dismutase (SOD), catalase (Cat), and glutathione peroxidase (GpX). The motility and viability of sperm decrease with Cd and improve with PBP and Sp, as the acrosomal reaction (AR) is diminished by PBPs. Testosterone levels decrease due to Cd, and only Sp maintains elevated levels. Cd increases the production of malondialdehyde in the spermatozoa, but not in testes; this production of malondialdehyde in the spermatozoa decreases in the presence of PBP. ROS only decreases with Cd, FBP, and Sp at high concentrations. Advanced oxidative protein products (AOPP) decrease with Cd and PBPs. Cat and GpX increase their activity with Cd and are altered by FBP. Cd produces vascular alterations testes. Within the seminiferous tubule, it produces areas of necrosis and apoptosis, which improve with PBPs and Sp. PBPs have a strong antioxidant activity as they show protective properties against Cd oxidative-induced toxicity on testes and sperm.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a50a588121265df2f64b31f6faac878Test
https://doi.org/10.1007/s11356-021-16668-3Test

4

Protective effect of selenium in prednisolone testicular toxicity

المؤلفون: Ahmad Ali Mohammadpour, Ali Mohammadeini

المصدر: Comparative Clinical Pathology. 30:847-856

مصطلحات موضوعية: chemistry.chemical_classification, medicine.medical_specialty, Hematology, Chemistry, chemistry.chemical_element, Testicle, Sperm, Pathology and Forensic Medicine, medicine.anatomical_structure, Enzyme, Endocrinology, Internal medicine, medicine, Prednisolone, Analysis of variance, Anatomy, Spermatogenesis, Selenium, medicine.drug

الوصف: The most common side effects of corticosteroids are adrenal suppression and the subsequent complications that can be attributed to testicular tissue degradation and dysfunction. Selenium or sodium selenite concentration indicates the protective role of this rare element and its related enzymes during spermatogenesis. The concentration of selenium in testis is regulated by a homeostatic mechanism that precedes the capacity of selenium in male gonads from other tissues. Therefore, the aim of this research was to investigate the protective effects of selenium on tissue structure and performance of testis in mice. In this research, 40 NMRI mice were treated in 5 groups (each including 8 mice) for 8 weeks under darkness–lightness conditions (12–12) at 25 C. The study groups were control group, prednisolone control group (1.5 mg/kg), prednisolone control group (2.5 mg/kg), and two treatment groups with selenium (0.2 mg/kg). Data resulting from investigation of sperm parameters and histological studies were analyzed using ANOVA software. The results showed that the group treated with sodium selenite along with prednisolone showed a significant decrease in testicle weight (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::cb5d63bbb2026fe88a5a0c49b88d3dfcTest
https://doi.org/10.1007/s00580-021-03276-3Test

5

The Effects of P‐glycoprotein Modulators on the Transition of Levofloxacin to Rat Brain, Testicle, and Plasma: In Vivo and In Silico Studies

المؤلفون: Kamil Uney, Gul Cetin, Bünyamin Traş

المصدر: ChemistrySelect. 6:7476-7481

مصطلحات موضوعية: biology, Transition (genetics), Chemistry, In silico, Biological activity, General Chemistry, Testicle, Rat brain, Molecular biology, medicine.anatomical_structure, In vivo, Levofloxacin, medicine, biology.protein, P-glycoprotein, medicine.drug

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::8d08c3b08f0d930b7bc94b962896d1a2Test
https://doi.org/10.1002/slct.202102122Test

6

Benzo-a-pyrene-induced reproductive toxicity was abated in rats co-treated with taurine

المؤلفون: Solomon E. Owumi, Eseroghene S. Najophe, Moses T. Otunla, Opeoluwa Popoola, Uche A. Okuu

المصدر: Toxin Reviews. 41:846-859

الوصف: Benzo-a-pyrene (BaP) exposure is toxic to the reproductive systems. The beneficial effects of taurine (TAU) against BaP-induced toxicity in the epididymis and testes of rats co-treated with BaP (10 mg/kg) and TAU at doses of 100 and 200 mg/kg for 28 successive days are reported in this study. Co-treatment with TAU significantly alleviated BaP-induced decreases in sperm quality, reproductive hormones, oxidative stress and inflammatory biomarkers. TAU also lessened epididymal and testicular injuries in rats. TAU ameliorated toxic responses in the epididymis and testes of rats exposed to BaP by abating oxidative and inflammatory responses, improved sperm quality and enhanced reproductive hormone levels.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e52741fbb880d464a5d982f05e67991Test
https://doi.org/10.1080/15569543.2021.1949617Test

7

THE EFFECT OF ADMINISTERING AFRICAN BITTER LEAF EXTRACT ON SPERMATOGENIC CELLS OF HYPERGLYCEMIC WISTAR RATS

المؤلفون: Kartana Kartana, Niluh Suwasanti, Paul L Tahalele

المصدر: Journal of Widya Medika Junior. 3:140-146

مصطلحات موضوعية: Spermatogenic Cell, Spermatogonium, Spermatid, business.industry, Physiology, Testicle, medicine.disease, Male infertility, Glibenclamide, chemistry.chemical_compound, medicine.anatomical_structure, chemistry, Diabetes mellitus, Alloxan, medicine, business, medicine.drug

الوصف: Introduction: Diabetes is still a world health problem that can cause many complications. Male infertility is one of the diabetic complications. This condition is caused by oxidative stress in diabetic patients. African bitter leaf is believed to contain an antioxidant compound that can repair male infertility. Purpose: This study aims to determine the effect of administering African bitter leaf extract on spermatogenic cell count in hyperglycemic Wistar rats. Method: This study use Rattus norvegicus as an animal model, which was administrated with alloxan to induce hyperglycemic. P0 group were given Na CMC 0,1%, Control group were given glibenclamide 0,63/kg bodyweight for 14 days. 100mg/kg bodyweight (P1), 200mg/kg (P2), and 400mg/kg (P3) of African bitter leaf extract were administrated for 14 days. In the end, the animals were sacrificed, and testicle histopathologic sections were made. Results: Significant result (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::b85165b6688ccb533ef37501c4018ee9Test
https://doi.org/10.33508/jwmj.v3i3.3337Test

8

Influence of freezing techniques and glycerol-based cryoprotectant combinations on the survival of testicular tissues from adult collared peccaries

المؤلفون: Andréia Maria da Silva, Alexandre Rodrigues Silva, Pierre Comizzoli, João Batista Freire Souza-Junior, Ana Gloria Pereira, Alexsandra Fernandes Pereira, Moacir Franco de Oliveira, Andreza Vieira Brasil, Luã Barbalho de Macêdo, Carlos Eduardo Bezerra de Moura

المصدر: Theriogenology. 167:111-119

الوصف: The objective of the study was to evaluate the effects of different cryopreservation techniques including glycerol-based cryoprotectant combinations on the structure and viability of testicular tissues from adult collared peccaries. Tissue biopsies (3.0 mm³) from 5 different individuals were allocated to 10 different groups: fresh control; slow freezing (SF), conventional vitrification (CV), or solid-surface vitrification (SSV); each of them using three different combinations of cryoprotectants [dimethyl sulfoxide (DMSO) + ethylene glycol (EG); DMSO + Glycerol; and EG + Glycerol]. After thawing/warming, samples were evaluated for histomorphology, viability, proliferative capacity potential, and DNA integrity. Most effective preservation of testicular histomorphology was achieved using SF and CV with DMSO + EG. However, the use of glycerol-based cryoprotectant combinations increased the occurrence of tubular cell swelling, tubular cell loss and shrinkage from the basal membrane. Cell viability was comparable among cryopreservation methods and cryoprotectant combinations. Regarding cell proliferative capacity, the use of SF with EG + Glycerol and SSV with DMSO + Glycerol impaired the conservation of spermatogonia proliferative potential compared to other treatments. Moreover, CV with DMSO + EG was better than SF with EG + Glycerol for Sertoli cell proliferation potential. Regarding DNA integrity, less damage occurred when using SF with DMSO + EG while more fragmentations were observed when using CV with EG + Glycerol or DMSO + Glycerol as well as SSV with EG + Glycerol or DMSO + Glycerol. In sum, SF and CV appeared to be the most suitable methods for the cryopreservation of adult peccary testicular tissues. Additionally, the use of glycerol-based cryoprotectant combinations did not improve testicular tissues preservation with DMSO + EG being the most efficient option.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa9e27c346d7e8bf5e57eda8dc8f3994Test
https://doi.org/10.1016/j.theriogenology.2021.03.013Test

9

Prebiotics mannan-oligosaccharides accelerate sexual maturity in rats: A randomized preclinical study

المؤلفون: Luiz Eduardo Rodrigues, Milena Miyoshi Kishibe, Rogeria Keller, Heliard Rodrigues dos Santos Caetano, Marcos Natal Rufino, Osimar de Carvalho Sanches, Ines Cristina Giometti, Rogério Giuffrida, Hermann Bremer-Neto

المصدر: Veterinary World
Veterinary World, Vol 14, Iss 5, Pp 1210-1219 (2021)

الوصف: Background and Aim: The prebiotics, mannan-oligosaccharides (MOS), demonstrate the ability to increase probiotic microorganisms and fixation and removal of pathogens associated with chronic systemic inflammation in the digestive system. Inflammatory processes play an important role in modulating the brain-intestinal axis, including maintaining male reproductive function and spermatogenesis and regulating stress. The aim of the present study was to evaluate the action of MOS on testosterone and corticosterone concentrations and the reproductive system development of rats in the growth phase as an animal model. Materials and Methods: In total, 128 male rats were used, randomly divided into four experimental groups (n=32): Control; MOS 1; MOS 2; and MOS 3. From each group, eight animals were sacrificed in four experimental moments (14, 28, 42, and 56 days, respectively, moments 1, 2, 3, and 4) and hormonal measurements and histological evaluations were performed. Results: The results revealed the effect of diet, MOS, and timing on testicle weight (p

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b4ffe8b182d4fa2917ce25667669c8fTest
https://doi.org/10.14202/vetworld.2021.1210-1219Test

10

miR-122-5p regulates the tight junction of the blood-testis barrier of mice via occludin

المؤلفون: Xiaoli Liu, Lumin Fei, Jianyun Shen, Li-Min Liu, Deyu Chen, Maoying Zhu

المصدر: Basic and Clinical Andrology, Vol 31, Iss 1, Pp 1-10 (2021)
Basic and Clinical Andrology

الوصف: Occludin protein is the primary assembling protein of TJs and the structural basis for tight junction formation between Sertoli cells in the spermatogenic epithelium. The expression of miR-122-5p and occludin are negatively correlated. In order to investigate the regulation mechanism of miR-122-5p on occludin and TJ, the present study isolated primary Sertoli cells from C57BL/6 mice, identified a transcription factor of miR-122-5p in testicle, studied the modulating loci of miR-122-5p on occludin using a dual-luciferase reporter assay, analyzed the regulate of miR-122-5p on the expression of occludin with real-time RT-PCR and Western blot, and studied the effect of miR-122-5p on the tight junction using a Millicell Electrical Resistance System.The relative luciferase activity in the pcDNA-Sp1 + pGL3-miR-122-5p promoter group was significantly higher than that in the pcDNA-Sp1 + pGL3-basic group, which suggests that transcript factor Sp1 promotes the transcription of miR-122-5p. The relative luciferase activity in the occludin 3'-UTR (wt) + miR-122-5p mimic group was significantly lower than that in the other groups (p 0.01), which indicates that miR-122-5p modulates the expression of occludin via the ACACTCCA sequence of the occludin-3'UTR. The levels of occludin mRNA and protein in the miR-122-5p mimic group were significantly lower than that in the other groups (p 0.05), which indicates that miR-122-5p reduces the expression of occludin. The trans-epithelial resistance of the miR-122-5p mimic group was significantly lower than that of the blank control group after day 4 (p 0.05), which indicates that miR-122-5p inhibited the assembly of the inter-Sertoli TJ permeability barrier in vitro.These results displayed that miR-122-5p could regulate tight junctions via the Sp1-miR-122-5p-occludin-TJ axis.CONTEXTE: La protéine occludine est. la principale protéine d’assemblage des jonctions serrées (JS), et la base structurelle pour la formation de ces jonctions entre les cellules de Sertoli dans l’épithélium séminifère. L’expression de miR-122-5p et de l’occludine sont négativement corrélées. Afin d’étudier le mécanisme de régulation de l’occludine et des TJ par miR-122-5p, nous avons, dans la présente étude, isolé des cellules primaires de Sertoli de souris C57BL/6, identifié un facteur de transcription de miR-122-5p dans le testicule, étudié les loci de miR-122-5p modulants l’occludine par le biais d’un système rapporteur à 2 luciférases, analysé la régulation de miR-122-5p sur l’expression de l’occludine par qRT-PCR et Western blot, et étudié l’effet de miR-122-5p sur les jonctions serrées à l’aide d’un Système de Résistance Electrique Millicell. RéSULTATS: L’activité relative de la luciférase dans le groupe du promoteur de pcDNA46 Sp1 + pGL3-miR-122-5p était significativement plus élevée que celle observée dans le groupe pcDNA-Sp1 + pGL3-basique, ce qui suggère que le facteur de transcription Sp1 favorise la transcription de miR-122-5p. L’activité relative de la luciférase dans le groupe 3′-UTR (wt) + miR-122-5p mimant l’occludine était significativement inférieure à celle des autres groupes (p 0,01), ce qui indique que miR-122-5p module l’expression de l’occludine via la séquence ACACTCCA en 3′ UTR de l’occludine. Les niveaux d’ARNm et de protéine occludine dans le groupe mimant miR-122-5p étaient significativement inférieurs à ceux des autres groupes (p 0,05), ce qui indique que miR-122-5p inhibe l’expression de l’occludine. La résistance transépithéliale du groupe mimant miR-122-5p était significativement inférieure à celle du groupe témoin vierge après le jour 4 (p 0.05), ce qui indique que miR-122-5p inhibe in vitro l’assemblage des jonctions serrées de la barrière de perméabilité inter-Sertolienne.Ces résultats montrent que miR-122-5p pourrait réguler les jonctions serrées via l’axe Sp1-miR-122-5p-occludine.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73469af1b0322ec542e8f8e496734033Test
https://doaj.org/article/3780e9ab57764ff294395157526a02a7Test

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