Redox Imbalance in CD4+ T Cells of Relapsing-Remitting Multiple Sclerosis Patients
| العنوان: | Redox Imbalance in CD4+ T Cells of Relapsing-Remitting Multiple Sclerosis Patients |
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| المؤلفون: | Mohammad Vodjgani, Abdorreza Naser Moghadasi, Mohammad Javad Tavassolifar, Omid Sadatpour, Maryam Izad, Behnaz Esmaeili |
| المصدر: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2020 (2020) |
| بيانات النشر: | Hindawi, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, CD4-Positive T-Lymphocytes, Male, Aging, medicine.medical_specialty, Multiple Sclerosis, Article Subject, NF-E2-Related Factor 2, education, SOD1, behavioral disciplines and activities, Biochemistry, Peripheral blood mononuclear cell, Superoxide dismutase, chemistry.chemical_compound, Recurrence, Superoxides, Internal medicine, mental disorders, medicine, Humans, chemistry.chemical_classification, Reactive oxygen species, NADPH oxidase, QH573-671, biology, Superoxide, Multiple sclerosis, CD28, Cell Biology, General Medicine, medicine.disease, Endocrinology, chemistry, Gene Expression Regulation, biology.protein, Female, Cytology, Oxidoreductases, Oxidation-Reduction, psychological phenomena and processes, Research Article |
| الوصف: | As a prevalent autoimmune disease of the central nervous system in young adults, multiple sclerosis (MS) is mediated by T cells, particularly CD4+ subsets. Given the evidence that the perturbation in reactive oxygen species (ROS) production has a pivotal role in the onset and progression of MS, its regulation through the antioxidant molecules is too important. Here, we investigated the level of the redox system components in lymphocytes and CD4+ T cells of MS patients. The study was performed on relapsing-remitting MS (RRMS) patients ( n = 29 ) and age- and sex-matched healthy controls ( n = 15 ). Peripheral blood mononuclear cells (PBMCs) were cultured and stimulated by anti-CD3/CD28. The level of ROS, anion superoxide (O2-), and L-𝛾-glutamyl-Lcysteinylglycine (GSH) was measured by flow cytometry in lymphocytes/CD4+ T cells. The gene expression level of gp91phox, catalase, superoxide dismutase 1/2 (SOD), and nuclear factor-E2-related factor (Nrf2) was also measured by real-time PCR. We found that lymphocytes/CD4+ T cells of RRMS patients at the relapse phase significantly produced higher levels of ROS and O2- compared to patients at the remission phase ( P value < 0.001) and healthy controls ( P value < 0.001 and P value < 0.05, respectively). Interestingly, the gene expression level of gp91phox, known as the catalytic subunit of the NADPH oxidase, significantly increased in MS patients at the relapse phase ( P value < 0.05). Furthermore, the catalase expression augmented in patients at the acute phase ( P value < 0.05), while an increased expression of SOD1 and Nrf2 was found in RRMS patients at relapse and remission phases ( P value < 0.05). The increased production of ROS in CD4+ T cells of RRMS patients highlights the importance of amplifying antioxidant components as an efficient approach to ameliorate disease activity in MS patients. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 1942-0994 1942-0900 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cd4a251eeee30eddbcb34067cad6b56Test http://europepmc.org/articles/PMC7735833Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2cd4a251eeee30eddbcb34067cad6b56 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19420994 19420900 |
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