Assessment of anti-inflammatory effects of Nitric Oxide administrated by inhalatory way on the experimental model of carrageenan-induced paw edema in mice
| العنوان: | Assessment of anti-inflammatory effects of Nitric Oxide administrated by inhalatory way on the experimental model of carrageenan-induced paw edema in mice |
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| المؤلفون: | Carly de Faria Coelho |
| المساهمون: | Rodrigo Alvaro Brandão Lopes Martins, Yara Cury, Zuleica Bruno Fortes, Carmen Penido Monteiro, Wilson da Costa Santos |
| المصدر: | Biblioteca Digital de Teses e Dissertações da USP Universidade de São Paulo (USP) instacron:USP |
| بيانات النشر: | Universidade de São Paulo, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Inhalation, business.industry, Sildenafil, Pharmacology, medicine.disease, Nitric oxide, chemistry.chemical_compound, chemistry, Pleurisy, Intensive care, Edema, Medicine, medicine.symptom, business, Reperfusion injury, Rolipram, medicine.drug |
| الوصف: | COELHO, C. F. Assessment of anti-inflammatory effects of Nitric Oxide administrated by inhalatory way on the experimental model of carrageenan-induced paw edema in mice. 102 p. Thesis Instituto de Ciencias Biomedicas da Universidade de Sao Paulo, Sao Paulo, 2009. The introduction of inhaled nitric oxide (iNO) for the treatment of many pulmonary injuries, as hypertension of the newborn, adult respiratory distress syndrome and lung reperfusion injury has been one of the most significant advances in recent intensive care. Inhaled NO (iNO) is qualified as "microselective" once it dilates only the vessels directly adjacent to the alveolar units being ventilated, but some evidences support that these pharmacological effects of iNO can reach other vasculature, beyond the pulmonary vasculature. Based on these findings, this study aimed to investigate the antiinflammatory effect of inhaled nitric oxide on the mice paw edema and pleurisy induced by carrageenan in mice and advance in knowledge of its possible mechanisms of action. Methods: Paw edema was induced on male Swiss mice (20 – 30 g) by the subplantar injection of carrageenin (CGN; 300 μg) under halothane anesthesia. Edema time-course (mL) was measured by plethysmometry until the maximum of 12 hs following CGN administration. Thirty minutes after CGN injection, some of the animal groups inhaled NO (300 ppm at variable doses, times and multiple administrations), while others received sildenafil (1 mg/Kg, i.p) or rolipram (3 mg/Kg, i.p.) with or without NO. At the end of experiments, the animals were sacrificed and the injected paws were removed to determine mieloperoxidase activity and histological analysis by microscopy. Pleurisy reaction was induced by intratoracic injection of carrageenan. After 4 hours, animals were sacrificed and the pleural cavity was washed with PBS and then the exsudate was collected for the assessment of inflammatory cells acumulation Data are presented as mean values ± SEM. Differences among the groups were analyzed by ANOVA followed by Student-Newman-Keuls test for multiple comparisons and for comparisons of 2 groups was used T-Student test. P values lower than 0.05 were taken as significant. Results: The 13.6 ppm inhalation of NO during 10 minutes reduced significantly the CGNinduced paw edema. The same dose on less time of inhalation did not show antinflammatory effect. We could observe a decrease in protein nitration after iNO administration on the parameters mentioned above. One administration of iNO on the greatest dose and time showed a good antiinflammatory effect iqual the groups that received more than one administration. Sildenafil and rolipram administrated isolatelly reduced edema more than treatment with iNO alone, but sildenafil potencialized the iNO effect when co-administrated together with iNO. Moreover, iNO, on the greatest dose and time of inhalation, reduced the inflammatory cells accumulation in pleural cavity on the pleurisy model. Conclusion: Inhaled NO was effective in reducing inflammatory signs such as edema and neutrophil infiltration by the 13,6 ppm, with one administration, during 10 minutes. The biochemical way of cGMP seems to be involved, in part, with the anti-inflammatory effect of iNO. |
| اللغة: | Portuguese |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d02819f9a281d37e2f8a2ba1940f958Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3d02819f9a281d37e2f8a2ba1940f958 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |