Influence of chemistry and fiber diameter of electrospun PLA, PCL and their blend membranes, intended as cell supports, on their biological behavior
| العنوان: | Influence of chemistry and fiber diameter of electrospun PLA, PCL and their blend membranes, intended as cell supports, on their biological behavior |
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| المؤلفون: | María Luisa González-Fernández, Guillermo Vilariño-Feltrer, Vega Villar-Suárez, Ana Vallés-Lluch, María Herrero-Herrero, Sara Alberdi-Torres, José Carlos Rodríguez-Hernández |
| المساهمون: | Anatomia y Anatomia Patologicas Comparadas, Facultad de Veterinaria |
| المصدر: | BULERIA. Repositorio Institucional de la Universidad de León instname Polymer Testing, Vol 103, Iss, Pp 107364-(2021) RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| بيانات النشر: | Elsevier |
| مصطلحات موضوعية: | Polymers and Plastics, macromolecular substances, Adipose tissue derived-mesenchymal stem cells, Regenerative medicine, chemistry.chemical_compound, Polylactic acid, Tissue engineering, medicine, Polymers and polymer manufacture, Anatomía, Electrospinning, Chondrogenic differentiation, Cartilage, Organic Chemistry, technology, industry, and agriculture, Biomaterial, equipment and supplies, Chondrogenesis, 2407.05 Cultivo de Tejidos, TP1080-1185, medicine.anatomical_structure, chemistry, PCL, 3109.01 Anatomía, Nanofiber, MAQUINAS Y MOTORES TERMICOS, PLA, Biomedical engineering |
| الوصف: | [EN] The prevalence of osteoarthritis, a degenerative cartilage disease that causes joint surface erosion and loss of mobility, emphasizes the need of producing a functional articular cartilage replacement. Tissue engineering has been the focus of recent research as a possible strategy for cartilage regeneration and repair. The most widely used technique for the manufacture of nanofibers is polymer electrospinning. Polylactic acid (PLA) and polycaprolactone (PCL) have been proved particularly suitable for nanofiber preparation, with many biomedical applications. The main aim of this work was to evaluate the behavior of adipose tissue-derived mesenchymal stem cells (ASCs) cultured on biomaterials of PLA, PCL and a combination of both (PLA/PCL), manufactured by electrospinning. We analyzed the bioactive properties of these cells in cultures on them, in terms of proliferation, adhesion, morphology, viability and differentiation. In addition, the influence of the thickness of the fibers in each biomaterial on these cellular characteristics was evaluated for their use in Cartilage Regenerative Medicine applications to promote chondrogenic differentiation. Depending on the parameter assessed, different results were obtained on each biomaterial. Using both polymers successful results on cellular viability were obtained, although in the case of PCL the cellular response in all the experiments was significantly better. As for the blends, positive outcomes were obtained, but they did not overtake the characteristics of PCL. Interestingly, ASCs were able to differentiate into chondrocytes without adding specific chondrogenic media in the three biomaterials tested. Moreover, a marked cell differentiation on PCL with 1.8 ¿m-fiber diameter and PLA/PCL blends was observed. These findings may play a key role in cartilage Regenerative Medicine and Tissue Engineering. This study was financially supported by the Spanish Ministerio de Economía y Competitividad through the BES-2016-078024 grant. |
| وصف الملف: | application/pdf |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84a37d8093e66a1a646aebabe6e95611Test https://www.sciencedirect.com/science/article/pii/S0142941821003093Test#! |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....84a37d8093e66a1a646aebabe6e95611 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |