Fibrosis in Chronic Hepatitis C: Correlation between Immunohistochemically-Assessed Virus Load with Steatosis and Cellular Iron Content
| العنوان: | Fibrosis in Chronic Hepatitis C: Correlation between Immunohistochemically-Assessed Virus Load with Steatosis and Cellular Iron Content |
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| المؤلفون: | Maha Mosaad, Ahmed Montasser, Ahmed El Ray, Olfat Hammam, Heba Khalil, Maha Akl, Raffat Atta, Ahmed Abdel Hadi, Ali El Hindawi, Amgad Anas, Valérie Paradis |
| المساهمون: | Internal project 95T of TBRI, Principal investigators Professor Dr Maha Akl and Ahmed EL Ray, Pathology Department of Beaujon Hospital, Clichy, France. |
| المصدر: | Open Access Macedonian Journal of Medical Sciences; Vol 4, No 4 (2016): Dec 15 (OAMJMS); 578-584 Open Access Macedonian Journal of Medical Sciences Open Access Macedonian Journal of Medical Sciences, Vol 4, Iss 4, Pp 578-584 (2016) Open Access Macedonian Journal of Medical Sciences; Vol. 4 No. 4 (2016): Dec 15 (OAMJMS); 578-584 |
| بيانات النشر: | ID Design 2012/DOOEL Skopje, Republic of Macedonia, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, Cirrhosis, Infectology, lcsh:Medicine, Inflammation, chemistry.chemical_compound, Basic Science, FISH, Fibrosis, medicine, NS3/NS4, Stage (cooking), HCC, Sirius Red, HCV, VEGF, VEGFR2, business.industry, lcsh:R, General Medicine, medicine.disease, chemistry, Immunohistochemistry, Medicine, Steatosis, medicine.symptom, business, Viral load |
| الوصف: | AIM: We aimed study impact of hepatocytic viral load, steatosis, and iron load on fibrosis in chronic hepatitis C and role of VEGF and VEGFR overexpression in cirrhotic cases in evolving HCC.MATERIAL AND METHODS: Total of 120 cases were included from TBRI and Beaujon Hospital as chronic hepatitis C (CHC), post-hepatitis C cirrhosis, and HCC. Cases of CHC were stained for Sirius red, Prussian blue and immunohistochemically (IHC) for HCV-NS3/NS4. HCC were stained IHC for VEGF and by FISH.RESULTS: Stage of fibrosis was significantly correlated with inflammation in CHC (P < 0.01). Noticed iron load did not correlate with fibrosis. Steatosis was associated with higher inflammation and fibrosis. The cellular viral load did not correlate with inflammation, steatosis or fibrosis. VEGF by IHC was significantly higher in cases of HCC when compared to cirrhotic group (P < 0.001). Amplification of VEGFR2 was confirmed in 40% of cases of HCC. Scoring of VEGF by IHC was the good indicator of VEGFR2 amplification by FISH (P < 0.005).CONCLUSION: Grade of inflammation is the factor affecting fibrosis in CHC. The degree of liver damage is not related to cellular viral load or iron load. Steatosis is associated with higher inflammation and fibrosis. VEGF by IHC is correlated with overexpression of VEGFR2 by FISH. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1857-9655 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1e0182a345f47df80285035b6eb4cdcTest http://www.id-press.eu/mjms/article/view/oamjms.2016.122Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d1e0182a345f47df80285035b6eb4cdc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18579655 |
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