التفاصيل البيبلوغرافية
| العنوان: |
Mapping native disulfide bonds at a proteome scale. |
| المؤلفون: |
Lu, Shan, Fan, Sheng-Bo, Yang, Bing, Li, Yu-Xin, Meng, Jia-Ming, Wu, Long, Li, Pin, Zhang, Kun, Zhang, Mei-Jun, Fu, Yan, Luo, Jincai, Sun, Rui-Xiang, He, Si-Min, Dong, Meng-Qiu |
| المصدر: |
Nature Methods; Apr2015, Vol. 12 Issue 4, p329-331, 3p |
| مصطلحات موضوعية: |
PROTEOMICS, DISULFIDES, CHEMICAL bonds, MASS spectrometry, PEPTIDES, MONOCLONAL antibodies, PROTEINS |
| مستخلص: |
We developed a high-throughput mass spectrometry method, pLink-SS (http://pfind.ict.ac.cn/software/pLink/2014/pLink-SS.htmlTest), for precise identification of disulfide-linked peptides. Using pLink-SS, we mapped all native disulfide bonds of a monoclonal antibody and ten standard proteins. We performed disulfide proteome analyses and identified 199 disulfide bonds in Escherichia coli and 568 in proteins secreted by human endothelial cells. We discovered many regulatory disulfide bonds involving catalytic or metal-binding cysteine residues. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
