دورية أكاديمية
Activation of microglia and chemokines in light-induced retinal degeneration
| العنوان: | Activation of microglia and chemokines in light-induced retinal degeneration |
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| المؤلفون: | Zhang, C, Shen, JK, Lam, TT, Zeng, HY, Chiang, SK, Yang, F, Tso, MOM |
| المساهمون: | Zhang, C (reprint author), Johns Hopkins Univ Hosp, Wilmer Eye Inst, Woods Bldg,Room 457,600 N Wolfe St, Baltimore, MD 21287 USA., Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Baltimore, MD 21205 USA., Univ So Calif, Doheny Eye Inst, Keck Sch Med, Dept Ophthalmol, Los Angeles, CA USA., Bausch & Lomb Inc, Pharmacol R&D, Rochester, NY USA., Beijing Univ, Ctr Eye, Beijing 100871, Peoples R China., Johns Hopkins Univ Hosp, Wilmer Eye Inst, Woods Bldg,Room 457,600 N Wolfe St, Baltimore, MD 21287 USA. |
| المصدر: | SCI |
| بيانات النشر: | molecular vision |
| سنة النشر: | 2005 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | MONOCYTE CHEMOATTRACTANT PROTEIN-1, CENTRAL-NERVOUS-SYSTEM, NEONATAL-RAT-BRAIN, REACTIVE MICROGLIA, ALZHEIMERS-DISEASE, MCP-1 EXPRESSION, ROYAL-COLLEGE, INJURY, CELLS, PHOTORECEPTORS |
| الوصف: | Purpose: Microglial cells, which are activated and recruited by chemokines, have been shown to play crucial roles in neuronal degenerations of the central nervous system (CNS). This study investigated the activation and migration of retinal microglial cells and expression of chemokines in retinas in light-induced photoreceptor degeneration in mice. Methods: Ninety-five Balb/cJ mice were kept in cyclic light for 1 week followed by dark adaptation for 48 h prior to light exposure of 3 h at 3.5 Klux. Animals were enthuanized at various times after light exposure. Terminal deoxynucleotidyl transferase-mediated dUTP nick end label (TUNEL) assay, rat-anti-mouse CD11b and 5D4 antibodies, isolectin-B4, and a chemokine-specific gene array were used to detect DNA fragmentation during retinal degeneration, to label retinal microglial cells, and to determine the expression of retinal chemokines and chemokine receptors, respectively. Reverse-transcriptase coupled polymerase chain reactions (RT-PCRs) were conducted on selected chemokine mRNAs to confirm the gene array findings. Results: After intense light exposure, TUNEL-positive cells were noted in the outer nuclear layer (ONL) of the retina at 3 h, and their presence were noticeably increased at 1 day but declined at 3 days and 7 days after light exposure. In contrast, CD11b- or isolectin-B4-positive cells were seen in the ONL as early as 6 h and their presence increased significantly at 1 day and 3 days after light exposure. These cells displayed a round or ovoid morphology at 6 h and 1 day but assumed a more ameboid configuration at 3 days. By 7 day, the number of the microglial cells declined in the ONL and they became ramified, and were present mostly in the subretinal space. 5D4-positive cells with large cell bodies were only noted at 3 day and 7 day but not earlier. With chemokine-specific gene array analysis, we identified four chemokines and two chemokine receptors showing significant increases in their gene expressions. Among them, monocyte chemoattractant ... |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 1090-0535 |
| العلاقة: | MOLECULAR VISION.2005,11,(105),887-895.; 1022068; http://hdl.handle.net/20.500.11897/401436Test; WOS:000233014000001 |
| الإتاحة: | https://doi.org/20.500.11897/401436Test https://hdl.handle.net/20.500.11897/401436Test |
| رقم الانضمام: | edsbas.918A79A4 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 10900535 |
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