التفاصيل البيبلوغرافية
| العنوان: |
Attenuation of renal fibrosis after unilateral ureteral obstruction in mice lacking the N-type calcium channel. |
| المؤلفون: |
Mishima, Keiichiro, Nakasatomi, Masao, Takahashi, Shunsuke, Ikeuchi, Hidekazu, Sakairi, Toru, Kaneko, Yoriaki, Hiromura, Keiju, Nojima, Yoshihisa, Maeshima, Akito |
| المصدر: |
PLoS ONE; 10/9/2019, Vol. 14 Issue 10, p1-15, 15p |
| مصطلحات موضوعية: |
RENAL fibrosis, URETERIC obstruction, CALCIUM channels, PROXIMAL kidney tubules, TYROSINE hydroxylase, MICE, MYOFIBROBLASTS, NERVE fibers |
| مستخلص: |
The N-type Ca2+ channel (Cav2.2) is distributed in sympathetic nerves that innervate the tubules, the vessels, and the juxtaglomerular granular cells of the kidney. However, the role of N-type Ca2+ channels in renal disease remains unknown. To address this issue, Cav2.2 knockout mice were utilized. Immunoreactive Cav2.2 was undetectable in normal kidneys of C57BL/6N mice, but it became positive in the interstitial S100-positive nerve fibers after unilateral ureteral obstruction (UUO). There were no significant differences in mean blood pressure, heart rate, and renal function between wild-type littermates and Cav2.2-knockout mice at baseline, as well as after UUO. Cav2.2 deficiency significantly reduced the EVG-positive fibrotic area, alpha-SMA expression, the production of type I collagen, and the hypoxic area in the obstructed kidneys. The expression of tyrosine hydroxylase, a marker for sympathetic neurons, was significantly increased in the obstructed kidneys of wild-type mice, but not in Cav2.2-knockout mice. These data suggest that increased Cav2.2 is implicated in renal nerve activation leading to the progression of renal fibrosis. Blockade of Cav2.2 might be a novel therapeutic approach for preventing renal fibrosis. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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