المؤلفون: Philippe Kastner, Corinne Bronn, Susan Chan, Andrée Dierich, Jonathan Back

المصدر: Blood. 103:3615-3623

مصطلحات موضوعية: Erythroblasts, Cellular differentiation, Green Fluorescent Proteins, Immunology, Regulator, Apoptosis, Mice, Transgenic, Biology, Biochemistry, Green fluorescent protein, Mice, Fetus, Proto-Oncogene Proteins, Animals, Erythropoiesis, Transcription factor, Cells, Cultured, Erythroid Precursor Cells, Mice, Knockout, Cell Differentiation, Cell Biology, Hematology, Cell biology, Luminescent Proteins, Haematopoiesis, Gene Expression Regulation, Trans-Activators, Stem cell, Signal transduction, Cell Division

الوصف: PU.1 is a hematopoietic-specific transcriptional activator that is absolutely required for the differentiation of B lymphocytes and myeloid-lineage cells. Although PU.1 is also expressed by early erythroid progenitor cells, its role in erythropoiesis, if any, is unknown. To investigate the relevance of PU.1 in erythropoiesis, we produced a line of PU.1-deficient mice carrying a green fluorescent protein reporter at this locus. We report here that PU.1 is tightly regulated during differentiation—it is expressed at low levels in erythroid progenitor cells and down-regulated upon terminal differentiation. Strikingly, PU.1-deficient fetal erythroid progenitors lose their self-renewal capacity and undergo proliferation arrest, premature differentiation, and apoptosis. In adult mice lacking one PU.1 allele, similar defects are detected following stress-induced erythropoiesis. These studies identify PU.1 as a novel and critical regulator of erythropoiesis and highlight the versatility of this transcription factor in promoting or preventing differentiation depending on the hematopoietic lineage.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84f0058671b94fa7fab92df49fafd7eaTest
https://doi.org/10.1182/blood-2003-11-4089Test

المؤلفون: Aleksandr Piskunov, Irwin Davidson, Bernard Jost, Sylvia Urban, Tao Ye, Cécile Rochette-Egly, Samia Gaouar, Ziad Al Tanoury, Andrée Dierich, Céline Keime

المصدر: Journal of cell science. 127(Pt 9)

مصطلحات موضوعية: Receptors, Retinoic Acid, Cellular differentiation, Response element, Retinoic acid, Cell Differentiation, Tretinoin, Cell Biology, Biology, Molecular biology, Embryonic stem cell, chemistry.chemical_compound, Retinoic acid receptor, Mice, chemistry, Nuclear receptor, Phosphorylation, Animals, Transcription factor, Embryonic Stem Cells

الوصف: Retinoic acid (RA) plays key roles in cell differentiation and growth arrest by activating nuclear receptors, RARs (α, β and γ), which are ligand dependent transcriptional factors. RARs are also phosphorylated in response to RA. Here we investigated the in vivo relevance of RARs phosphorylation during RA-induced neuronal differentiation of mouse embryonic stem (mES) cells. Using ES cells where the genes encoding each RAR subtype have been inactivated and stable rescue lines expressing RARs mutated in phosphor-acceptor sites, we show that RA-induced neuronal differentiation involves RARγ2 and requires RARγ2 phosphorylation. By gene expression profiling, we found that the phosphorylated form of RARγ2 regulates a small subset of genes through binding an unusual RA response element consisting of two direct repeats with a 7 base pair spacer. These new findings suggest an important role for RARγ phosphorylation during cell differentiation, and pave the way for further investigations during embryonic development.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4cbbbc9969a54472534a7b39e00c439Test
https://pubmed.ncbi.nlm.nih.gov/24569880Test

المؤلفون: Beatrice Reinhardt, David Duval, Hélène Boeuf, Maria Zeniou, Yolande Huss-Garcia, Karine Merienne, Charlotte Hauss, Andrée Dierich, David A. Frank, André Hanauer, Claude Kedinger, Sylvie Jacquot

المصدر: Journal of Biological Chemistry. 276:46204-46211

مصطلحات موضوعية: STAT3 Transcription Factor, endocrine system, Cellular differentiation, Apoptosis, CREB, Leukemia Inhibitory Factor, Biochemistry, Mice, Animals, Phosphorylation, CREB-binding protein, STAT3, Molecular Biology, reproductive and urinary physiology, Lymphokines, biology, Interleukin-6, urogenital system, Ribosomal Protein S6 Kinases, Stem Cells, Nuclear Proteins, Cell Differentiation, Cell Biology, Embryo, Mammalian, CREB-Binding Protein, Embryonic stem cell, Molecular biology, Growth Inhibitors, Cell biology, DNA-Binding Proteins, embryonic structures, Trans-Activators, biology.protein, Mitogen-Activated Protein Kinases, Stem cell, Leukemia inhibitory factor, hormones, hormone substitutes, and hormone antagonists, Signal Transduction

الوصف: Mouse embryonic stem (ES) cells remain "pluripotent" in vitro in the continuous presence of leukemia inhibitory factor (LIF). In the absence of LIF, ES cells are irreversibly committed to differentiate into various lineages. In this study we have set up an in vitro assay based on the anti-apoptotic activity of LIF to distinguish pluripotent from "differentiation-committed" ES cells. We have examined the phosphorylation profiles of known (STAT3 and ERKs) and identified new (ribosomal S6 kinases (RSKs) and cAMP-responsive element-binding protein (CREB)) LIF-regulated targets in ES and in ES-derived neuronal cells. We have demonstrated that although STAT3, a crucial player in the maintenance of ES cell pluripotency, is induced by LIF in all cell types tested, the LIF-dependent activation of RSKs is restricted to ES cells. We have shown that LIF-induced phosphorylation of RSKs in ES cells is dependent on ERKs, whereas STAT3 phosphorylation is not mediated by any known MAPK activities. Our results also demonstrate that the LIF-dependent phosphorylation of CREB is partially under the control of the RSK2 kinase.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29103b73e761efe7a4fffb4ae9183328Test
https://doi.org/10.1074/jbc.m106718200Test

المؤلفون: Evelyne Moreau, Pirjo Pakarinen, Stéphanie Migrenne, Jorge Merlet, René Habert, Andrée Dierich, Chrystèle Racine

المساهمون: Gametogenèse et génotoxicité (UMR_S_566 ), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Diderot - Paris 7 (UPD7)-IFR13-Institut National de la Santé et de la Recherche Médicale (INSERM)

المصدر: PLoS ONE
PLoS ONE, 2012, 7 (12), pp.e53257. ⟨10.1371/journal.pone.0053257⟩
PLoS ONE, Vol 7, Iss 12, p e53257 (2012)

مصطلحات موضوعية: Male, Anatomy and Physiology, Mouse, Cellular differentiation, Follicle-stimulating hormone, Mice, 0302 clinical medicine, Endocrinology, Reproductive Physiology, Testis, Morphogenesis, Testosterone, Receptor, Mice, Knockout, 0303 health sciences, Multidisciplinary, Sexual Differentiation, Leydig Cells, Animal Models, Sertoli cell, medicine.anatomical_structure, Receptors, FSH, Medicine, hormones, hormone substitutes, and hormone antagonists, Signal Transduction, Research Article, medicine.medical_specialty, Cell Physiology, endocrine system, Science, 030209 endocrinology & metabolism, Endocrine System, Biology, Paracrine Mechanisms, ta3111, 03 medical and health sciences, Paracrine signalling, Model Organisms, Internal medicine, medicine, Animals, Reproductive Endocrinology, Desert hedgehog, 030304 developmental biology, Sertoli Cells, Endocrine Physiology, Reproductive System, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, Follicle Stimulating Hormone, Hormone, Developmental Biology

الوصف: It is currently admitted that Follicle-Stimulating Hormone (FSH) is physiologically involved in the development and function of fetal/neonatal Sertoli cells in the rat but not the mouse. However, FSH is produced by both species from late fetal life onwards. We thus reinvestigated the role of FSH in mouse testis development at day 0 (birth) 6, 8 and 10 post-partum (dpp) by using mice that lack functional FSH receptors (FSH-R(-/-)). At birth, the number and proliferative index of Sertoli cells were significantly lower in FSH-R(-/-) mice than in wild type neonates. Claudin 11 mRNA expression also was significantly reduced in FSH-R(-/-) testes at 0 and 8 dpp, whereas the mRNA levels of other Sertoli cell markers (Transferrin and Desert hedgehog) were comparable in FSH-R(-/-) and wild type testes. Conversely, AMH mRNA and protein levels were higher at birth, comparable at 6 dpp and then significantly lower in FSH-R(-/-) testes at 8-10 dpp in FSH-R(-/-) mice than in controls. Although the plasma concentration of LH and the number of Leydig cells were similar in FSH-R(-/-) and control (wild type), testosterone concentration and P450c17 mRNA expression were significantly increased in FSH-R(-/-) testes at birth. Conversely, at 10 dpp when adult Leydig cells appear, expression of the steroidogenic genes P450scc, P450c17 and StAR was lower in FSH-R(-/-) testes than in controls. In conclusion, our results show that 1) like in the rat, signaling via FSH-R controls Sertoli cell development and function during late fetal life in the mouse as well; 2) paracrine factors produced by Sertoli cells are involved in the FSH-R-dependent regulation of the functions of fetal Leydig cells in late fetal life; and 3) the role of FSH-R signaling changes during the prepubertal period.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5e5c5d1a972ead608f5152cdddbca5dTest
https://hal.science/hal-03852274Test

المؤلفون: Hélène Haegel, Andrée Dierich, Rhodri Ceredig

المصدر: Developmental Immunology
Clinical and Developmental Immunology, Vol 3, Iss 4, Pp 239-246 (1994)

مصطلحات موضوعية: lcsh:Immunologic diseases. Allergy, Cellular differentiation, Immunology, Receptors, Lymphocyte Homing, Receptors, Cell Surface, Cell Line, Exon, Mice, Animals, adhesion molecules, Northern blot, RNA, Messenger, CD44, biology, Stem Cells, Alternative splicing, Cell Differentiation, embryonic stem cells, Molecular biology, Embryonic stem cell, Cell biology, Alternative Splicing, Hyaluronan Receptors, Cell culture, Antigens, Surface, biology.protein, Stem cell, lcsh:RC581-607, Carrier Proteins, Developmental Biology, Research Article

الوصف: Expression of the surface-adhesion molecule CD44 was investigated during thein vitrodifferentiation of the embryonic stem (ES) cell line D3. By immunofluorescence analysis, totipotent, undifferentiated ES cells did not show surface expression of CD44, although two transcripts of approximately 1.6 and 3.3 kb were detected on Northern blots. Following 1 week of differentiation in either suspension or substrate-attached cultures, CD44 appeared on the surface of some D3 cells, and synthesis of an additional 4.5 kb mRNA species was detected on Northern blots. At this stage, at least three distinct transcripts encoding CD44 variants were induced within the cultures, resulting from alternative splicing of additional exons in the variable domains of CD44. From PCR analysis, they all appeared to contain the variable exon v10, and two of them in addition contained v6. Taken together, these results suggest that CD44 may play a role in cell migration and adhesion in the early development of the mouse embryo.

وصف الملف: text/xhtml

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c9aa368d551d4a6298b12fc5fc22973Test
http://europepmc.org/articles/PMC2275938Test

المؤلفون: Carlos Mendoza-Palomares, Christina Cortez, N. Messadeq, Angus P. R. Johnston, Nadia Benkirane-Jessel, Jean-Claude Voegel, Didier Mainard, Sybille Facca, Frank Caruso, Andrée Dierich

المصدر: Proceedings of the National Academy of Sciences of the United States of America. 107(8)

مصطلحات موضوعية: Cellular differentiation, Bone Morphogenetic Protein 2, Capsules, Bone morphogenetic protein 2, Cell Line, Mice, Tissue engineering, In vivo, Osteogenesis, Bone cell, Animals, Regeneration, Polylysine, Embryonic Stem Cells, Multidisciplinary, Osteoblasts, Tissue Engineering, Chemistry, Regeneration (biology), Cell Differentiation, Transforming Growth Factor alpha, Biological Sciences, Embryonic stem cell, Cell biology, Polyglutamic Acid, Immunology, Stem cell

الوصف: Interest in the development of new sources of transplantable materials for the treatment of injury or disease has led to the convergence of tissue engineering with stem cell technology. Bone and joint disorders are expected to benefit from this new technology because of the low self-regenerating capacity of bone matrix secreting cells. Herein, the differentiation of stem cells to bone cells using active multilayered capsules is presented. The capsules are composed of poly-L-glutamic acid and poly-L-lysine with active growth factors embedded into the multilayered film. The bone induction from these active capsules incubated with embryonic stem cells was demonstrated in vitro. Herein, we report the unique demonstration of a multilayered capsule-based delivery system for inducing bone formation in vivo. This strategy is an alternative approach for in vivo bone formation. Strategies using simple chemistry to control complex biological processes would be particularly powerful, as they make production of therapeutic materials simpler and more easily controlled.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16eb3218b6e98ad5eaf4266415b75acdTest
https://pubmed.ncbi.nlm.nih.gov/20160118Test

المؤلفون: Diane Mathis, Dominic Cosgrove, Marianne LeMeur, Christophe Benoist, James C. Kaufman, Andrée Dierich, David Gray

المصدر: Cell. 66:1051-1066

مصطلحات موضوعية: Antigens, Differentiation, T-Lymphocyte, CD8 Antigens, T-Lymphocytes, Cellular differentiation, Receptors, Lymphocyte Homing, Thymus Gland, Gene Rearrangement, T-Lymphocyte, General Biochemistry, Genetics and Molecular Biology, Mice, Antigen, T-Lymphocyte Subsets, medicine, Animals, B cell, B-Lymphocytes, MHC class II, biology, Homozygote, Histocompatibility Antigens Class II, Lymphocyte differentiation, Germinal center, Cell Differentiation, T lymphocyte, Flow Cytometry, Mice, Mutant Strains, Cell biology, B-1 cell, medicine.anatomical_structure, Antibody Formation, CD4 Antigens, Immunology, biology.protein, Lymph Nodes, Spleen

الوصف: We have produced mice that lack major histocompatibility complex class II antigens, permitting us to evaluate the role of these molecules in diverse aspects of T and B cell differentiation. The mutant mice show near-complete elimination of CD4+ T lymphocytes from the spleen and lymph nodes; the few remaining CD4-positive cells are preferentially localized to B cell follicles. Surprisingly, substantial numbers of CD4 single-positive cells reside in the thymus; however, these are not mature thymocytes as we currently recognize them. B lymphocytes occur in normal numbers and are capable of terminal differentiation to plasma cells. Nevertheless, several aberrations in the B cell compartment are demonstrable: a lack of germinal centers, fewer IgM+IgD+ cells in certain individuals, reduced production of serum IgG1, and complete inability to respond to T-dependent antigens. In short, the class II-negative mice have confirmed some old ideas about lymphocyte differentiation, but have provided some surprises.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a198245e1459d910a7652305ccc4711bTest
https://doi.org/10.1016/0092-8674Test(91)90448-8

المؤلفون: Nadia Messaddeq, Canan G. Nebigil, Kyoji Urayama, Selçuk Takır, Hitoshi Kurose, Célia Guilini, Gulen Turkeri, Andrée Dierich

المساهمون: Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Clinique de la Souris (ICS), Intégrité du génome, Ecole Supérieure de Biotechnologie de Strasbourg (ESBS), Université de Strasbourg (UNISTRA)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des biomolécules (LBM UMR 7203), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Chimie Moléculaire de Paris Centre (FR 2769), Institut de Chimie du CNRS (INC)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I

المصدر: Arteriosclerosis, Thrombosis, and Vascular Biology
Arteriosclerosis, Thrombosis, and Vascular Biology, American Heart Association, 2008, 28 (5), pp.841-9. ⟨10.1161/ATVBAHA.108.162404⟩
Arteriosclerosis, Thrombosis, and Vascular Biology, American Heart Association, 2008, 28 (5), pp.841-849. ⟨10.1161/ATVBAHA.108.162404⟩

مصطلحات موضوعية: MESH: Signal Transduction, MESH: Pericardium, Angiogenesis, Cellular differentiation, MESH: Myocytes, Cardiac, 030204 cardiovascular system & hematology, MESH: Neuropeptides, Muscle, Smooth, Vascular, Mice, 0302 clinical medicine, Cell Movement, MESH: Up-Regulation, Myocytes, Cardiac, MESH: Animals, MESH: Gastrointestinal Hormones, MESH: Cell Movement, Protein Kinase C, ComputingMilieux_MISCELLANEOUS, 0303 health sciences, Stem Cells, Cell Differentiation, MESH: Muscle, Smooth, Vascular, Prokineticin, Cell biology, Up-Regulation, MESH: Endothelium, Vascular, Cardiology and Cardiovascular Medicine, Pericardium, MESH: Neovascularization, Physiologic, Signal Transduction, MESH: Cell Differentiation, medicine.medical_specialty, MESH: Mice, Transgenic, Transgene, Neovascularization, Physiologic, Mice, Transgenic, MESH: Stem Cells, Biology, Gastrointestinal Hormones, MESH: Coculture Techniques, 03 medical and health sciences, Paracrine signalling, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Internal medicine, MESH: Cell Proliferation, medicine, Animals, Progenitor cell, Autocrine signalling, MESH: Mice, 030304 developmental biology, Cell Proliferation, Neuropeptides, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Prokineticin receptor 1, MESH: Protein Kinase C, Coculture Techniques, Endocrinology, Endothelium, Vascular

الوصف: Objective— Identification of novel factors that contribute to myocardial repair and collateral vessel growth hold promise for treatment of heart diseases. We have shown that transient prokineticin receptor-1 (PKR1) gene transfer protects the heart against myocardial infarction in a mouse model. Here, we investigated the role of excessive PKR1 signaling in heart. Methods and Results— Transgenic mice overexpressing PKR1 in cardiomyocytes displayed no spontaneous abnormalities in cardiomyocytes but showed an increased number of epicardial-derived progenitor cells (EPDCs), capillary density, and coronary arterioles. Coculturing EPDCs with H9c2 cardiomyoblasts overexpressing PKR1 promotes EPDC differentiation into endothelial and smooth muscle cells, mimicking our transgenic model. Overexpressing PKR1 in H9c2 cardiomyoblasts or in transgenic hearts upregulated prokineticin-2 levels. Exogenous prokineticin-2 induces significant outgrowth from neonatal and adult epicardial explants, promoting EPDC differentiation. These prokineticin-2 effects were abolished in cardiac explants from mice with PKR1-null mutation. Reduced capillary density and prokineticin-2 levels in PKR1-null mutant hearts supports the hypothesis of an autocrine/paracrine loop between PKR1 and prokineticin-2. Conclusion— Cardiomyocyte-PKR1 signaling upregulates its own ligand prokineticin-2 that acts as a paracrine factor, triggering EPDCs proliferation/differentiation. This study provides a novel insight for possible therapeutic strategies aiming at restoring pluripotency of adult EPDCs to promote neovasculogenesis by induction of cardiomyocyte PKR1 signaling.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a66360a520d3842486709223df910c0Test
https://hal.archives-ouvertes.fr/hal-00282980Test

المؤلفون: Nadia Messaddeq, Julien Vermot, Karen Niederreither, Pascal Dollé, Andrée Dierich

المساهمون: Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

المصدر: Differentiation / The Journal of the International Society of Differentiation
Differentiation / The Journal of the International Society of Differentiation, 2006, 74 (9-10), pp.661-8. ⟨10.1111/j.1432-0436.2006.00094.x⟩

مصطلحات موضوعية: MESH: Aldehyde Oxidoreductases, MESH: Signal Transduction, Cancer Research, Cellular differentiation, Retinoic acid, MESH: Myocytes, Cardiac, Germline, chemistry.chemical_compound, Mice, 0302 clinical medicine, MESH: Embryonic Development, MESH: Animals, Myocytes, Cardiac, Transgenes, MESH: Embryonic Stem Cells, MESH: Chimera, 0303 health sciences, Cell Differentiation, Aldehyde Oxidoreductases, embryonic structures, medicine.drug, Signal Transduction, MESH: Cell Differentiation, MESH: Abnormalities, Multiple, MESH: Mutation, MESH: Mice, Transgenic, Morphogenesis, Embryonic Development, MESH: Transgenes, Mice, Transgenic, Tretinoin, Biology, Chimerism, 03 medical and health sciences, Chimera (genetics), medicine, Animals, Abnormalities, Multiple, Molecular Biology, MESH: Mice, Embryonic Stem Cells, 030304 developmental biology, MESH: Tretinoin, Chimera, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Cell Biology, MESH: Chimerism, Embryonic stem cell, Molecular biology, MESH: Blastocyst, Blastocyst, chemistry, Cell culture, Mutation, 030217 neurology & neurosurgery, Developmental Biology

الوصف: International audience; Retinoic acid (RA), the active vitamin A derivative, is an important developmental signaling molecule in vertebrates. In this study, we have assessed whether minimal numbers and/or specific distributions of RA-producing cells can support normal mouse embryonic development. Retinaldehyde dehydrogenase 2 (RALDH2) is the main RA-synthesizing enzyme acting during development. We have generated an embryonic stem (ES) cell line homozygous for an Raldh2 gene disruption, and have analyzed chimeric embryos with various contributions of wild-type cells. Whereas embryos almost completely derived from Raldh2(-/-) cells phenocopy the corresponding germline null mutants, the presence of even small numbers (

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::193c21724cb1c6eea4caed21da27f178Test
https://pubmed.ncbi.nlm.nih.gov/17177861Test

المؤلفون: Doulaye Dembélé, David Duval, Christelle Thibault, Beatrice Reinhardt, F. Diemunsch, A. L. Mertz, Marina Trouillas, Andrée Dierich, Hélène Boeuf

المساهمون: Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, Composantes innées de la réponse immunitaire et différenciation (CIRID), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Université de Perpignan Via Domitia (UPVD), Muniesa, Annie

المصدر: Cell Death and Differentiation
Cell Death and Differentiation, Nature Publishing Group, 2006, 13 (4), pp.564-75. ⟨10.1038/sj.cdd.4401789⟩
Cell Death and Differentiation, Nature Publishing Group, 2006, 13-4, pp.564-575

مصطلحات موضوعية: MAPK/ERK pathway, Cellular differentiation, Cell, Apoptosis, Stem cell marker, Leukemia Inhibitory Factor, p38 Mitogen-Activated Protein Kinases, Mice, 0302 clinical medicine, MESH: Gene Expression Regulation, Developmental, MESH: Animals, Enzyme Inhibitors, ComputingMilieux_MISCELLANEOUS, 0303 health sciences, Stem Cells, Imidazoles, Gene Expression Regulation, Developmental, Cell Differentiation, Cell biology, medicine.anatomical_structure, Proto-Oncogene Proteins c-bcl-2, MESH: Enzyme Inhibitors, 030220 oncology & carcinogenesis, MESH: Pluripotent Stem Cells, MESH: Imidazoles, Pluripotent Stem Cells, MESH: Cell Differentiation, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], p38 mitogen-activated protein kinases, MESH: Stem Cells, Biology, Transfection, Cell Line, 03 medical and health sciences, MESH: Gene Expression Profiling, medicine, Animals, Molecular Biology, MESH: Mice, 030304 developmental biology, Interleukin-6, [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], Gene Expression Profiling, MESH: Apoptosis, MESH: Transfection, MESH: Metallothionein, MESH: Embryo, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Cell Biology, MESH: Leukemia Inhibitory Factor, Embryo, Mammalian, Molecular biology, Embryonic stem cell, MESH: Interleukin-6, MESH: Cell Line, MESH: p38 Mitogen-Activated Protein Kinases, MESH: Proto-Oncogene Proteins c-bcl-2, Metallothionein, Leukemia inhibitory factor

الوصف: International audience; Mouse embryonic stem (ES) cells remain pluripotent in vitro when grown in the presence of leukemia inhibitory factor (LIF). LIF starvation leads to apoptosis of some of the ES-derived differentiated cells, together with p38alpha mitogen-activated protein kinase (MAPK) activation. Apoptosis, but not morphological cell differentiation, is blocked by a p38 inhibitor, PD169316. To further understand the mechanism of action of this compound, we have identified its specific targets by microarray studies. We report on the global expression profiles of genes expressed at 3 days upon LIF withdrawal (d3) compared to pluripotent cells and of genes whose expression is modulated at d3 under anti-apoptotic conditions. We showed that at d3 without LIF cells express, earlier than anticipated, specialized cell markers and that when the apoptotic process was impaired, expression of differentiation markers was altered. In addition, functional tests revealed properties of anti-apoptotic proteins not to alter cell pluripotency and a novel role for metallothionein 1 gene, which prevents apoptosis of early differentiated cells.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2f541004ec90a1ebdbad6871a8e5a09Test
https://hal.archives-ouvertes.fr/hal-00187902Test