التفاصيل البيبلوغرافية
| العنوان: |
In Vivo Optical Imaging of Tumor and Microvascular Response to Ionizing Radiation. |
| المؤلفون: |
Maeda, Azusa, Leung, Michael K. K., Conroy, Leigh, Yonghong Chen, Bu, Jiachuan, Lindsay, Patricia E., Mintzberg, Shani, Virtanen, Carl, Tsao, Julissa, Winegarden, Neil A., Yanchun Wang, Morikawa, Lily, Vitkin, I. Alex, Jaffray, David A., Hill, Richard P., DaCosta, Ralph S., Bogyo, Matthew |
| المصدر: |
PLoS ONE; Aug2012, Vol. 7 Issue 8, Special section p1-15, 15p |
| مصطلحات موضوعية: |
RADIOTHERAPY, CANCER treatment, IONIZING radiation, TUMORS, CELLS, PROTEINS |
| مستخلص: |
Radiotherapy is a widely used cancer treatment. However, understanding how ionizing radiation affects tumor cells and their vasculature, particularly at cellular, subcellular, genetic, and protein levels, has been limited by an inability to visualize the response of these interdependent components within solid tumors over time and in vivo. Here we describe a new preclinical experimental platform combining intravital multimodal optical microscopy for cellular-level longitudinal imaging, a small animal x-ray microirradiator for reproducible spatially-localized millimeter-scale irradiations, and laser- capture microdissection of ex vivo tissues for transcriptomic profiling. Using this platform, we have developed new methods that exploit the power of optically-enabled microscopic imaging techniques to reveal the important role of the tumor microvasculature in radiation response of tumors. Furthermore, we demonstrate the potential of this preclinical platform to study quantitatively-- with cellular and sub-cellular details--the spatio-temporal dynamics of the biological response of solid tumors to ionizing radiation in vivo. [ABSTRACT FROM AUTHOR] |
|
Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder