المؤلفون: Carolina Camacho-Fernández, Jose M. Seguí-Simarro, Ricardo Mir, Kim Boutilier, Patricia Corral-Martínez

المصدر: Frontiers in Plant Science, Vol 12 (2021)

مصطلحات موضوعية: androgenesis, arabinogalactan proteins, callose, cell wall, cellulose, microspore embryogenesis, Plant culture, SB1-1110

الوصف: Microspore cultures generate a heterogeneous population of embryogenic structures that can be grouped into highly embryogenic structures [exine-enclosed (EE) and loose bicellular structures (LBS)] and barely embryogenic structures [compact callus (CC) and loose callus (LC) structures]. Little is known about the factors behind these different responses. In this study we performed a comparative analysis of the composition and architecture of the cell walls of each structure by confocal and quantitative electron microscopy. Each structure presented specific cell wall characteristics that defined their developmental fate. EE and LBS structures, which are responsible for most of the viable embryos, showed a specific profile with thin walls rich in arabinogalactan proteins (AGPs), highly and low methyl-esterified pectin and callose, and a callose-rich subintinal layer not necessarily thick, but with a remarkably high callose concentration. The different profiles of EE and LBS walls support the development as suspensorless and suspensor-bearing embryos, respectively. Conversely, less viable embryogenic structures (LC) presented the thickest walls and the lowest values for almost all of the studied cell wall components. These cell wall properties would be the less favorable for cell proliferation and embryo progression. High levels of highly methyl-esterified pectin are necessary for wall flexibility and growth of highly embryogenic structures. AGPs seem to play a role in cell wall stiffness, possibly due to their putative role as calcium capacitors, explaining the positive relationship between embryogenic potential and calcium levels.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fpls.2021.737139/fullTest; https://doaj.org/toc/1664-462XTest

الوصول الحر: https://doaj.org/article/2e7aeb004f594c958fd77a086225845dTest

المؤلفون: Jose M. Seguí-Simarro, Carolina Camacho-Fernández, Kim Boutilier, Patricia Corral-Martínez, Ricardo Mir

المصدر: Frontiers in Plant Science, 12
Frontiers in Plant Science 12 (2021)
Frontiers in Plant Science, Vol 12 (2021)
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname

مصطلحات موضوعية: 0106 biological sciences, food.ingredient, Pectin, BIOLOGIA CELULAR, Plant Science, subintinal layer, 01 natural sciences, SB1-1110, Cell wall, 03 medical and health sciences, chemistry.chemical_compound, food, Microspore, Arabinogalactan, Microspore embryogenesis, Subintinal layer, Cell totipotency, BIOS Plant Development Systems, 030304 developmental biology, 0303 health sciences, Cell growth, cell totipotency, Callose, Plant culture, Embryo, cellulose, Cell biology, GENETICA, androgenesis, chemistry, Callus, microspore embryogenesis, cell wall, arabinogalactan proteins, callose, 010606 plant biology & botany

الوصف: [EN] Microspore cultures generate a heterogeneous population of embryogenic structures that can be grouped into highly embryogenic structures [exine-enclosed (EE) and loose bicellular structures (LBS)] and barely embryogenic structures [compact callus (CC) and loose callus (LC) structures]. Little is known about the factors behind these different responses. In this study we performed a comparative analysis of the composition and architecture of the cell walls of each structure by confocal and quantitative electron microscopy. Each structure presented specific cell wall characteristics that defined their developmental fate. EE and LBS structures, which are responsible for most of the viable embryos, showed a specific profile with thin walls rich in arabinogalactan proteins (AGPs), highly and low methyl-esterified pectin and callose, and a callose-rich subintinal layer not necessarily thick, but with a remarkably high callose concentration. The different profiles of EE and LBS walls support the development as suspensorless and suspensor-bearing embryos, respectively. Conversely, less viable embryogenic structures (LC) presented the thickest walls and the lowest values for almost all of the studied cell wall components. These cell wall properties would be the less favorable for cell proliferation and embryo progression. High levels of highly methyl-esterified pectin are necessary for wall flexibility and growth of highly embryogenic structures. AGPs seem to play a role in cell wall stiffness, possibly due to their putative role as calcium capacitors, explaining the positive relationship between embryogenic potential and calcium levels.
This work was supported by grant PID2020-115763RBI00 to JS-S from Spanish MICINN and by a Juan de la Cierva -Incorporacion Fellowship and a Marie Sklodowska-Curie Individual Fellowship (656579) to PC-M. RM holds a CDEIGENT (2018/023) fellowship from Generalitat Valenciana.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22d8f6d076a04877691de1cc496c8faaTest
https://doi.org/10.3389/fpls.2021.737139Test

المؤلفون: Jose M. Muiño, Hui Li, Tjitske Riksen, Mercedes Soriano, Jan H.G. Cordewener, Kim Boutilier, Hiroyuki Fukuoka, Gerco C. Angenent

المصدر: The Plant Cell, 26(1), 195-209
The Plant Cell
The Plant Cell 26 (2014) 1

مصطلحات موضوعية: Somatic embryogenesis, medicine.drug_class, Arabidopsis, Plant Science, arabidopsis-thaliana, in-vitro, retinoblastoma protein, Hydroxamic Acids, PRI BIOS Applied Genomics & Proteomics, Histones, BIOS Applied Bioinformatics, Cell Wall, medicine, Arabidopsis thaliana, Laboratorium voor Moleculaire Biologie, brassica-napus l, BIOS Plant Development Systems, plant-cell cycle, Research Articles, Cell Proliferation, Genetics, Gametophyte, biology, EPS-1, Indoleacetic Acids, Arabidopsis Proteins, Histone deacetylase inhibitor, fungi, food and beverages, Embryo, embryo development, Acetylation, Cell Biology, somatic embryogenesis, biology.organism_classification, Cell biology, Histone Deacetylase Inhibitors, Trichostatin A, Histone, biology.protein, microspore embryogenesis, Pollen, Laboratory of Molecular Biology, polycomb-group proteins, Ploidy, pollen embryogenesis, Cell Division, medicine.drug, Signal Transduction

الوصف: The haploid male gametophyte, the pollen grain, is a terminally differentiated structure whose function ends at fertilization. Plant breeding and propagation widely use haploid embryo production from in vitro–cultured male gametophytes, but this technique remains poorly understood at the mechanistic level. Here, we show that histone deacetylases (HDACs) regulate the switch to haploid embryogenesis. Blocking HDAC activity with trichostatin A (TSA) in cultured male gametophytes of Brassica napus leads to a large increase in the proportion of cells that switch from pollen to embryogenic growth. Embryogenic growth is enhanced by, but not dependent on, the high-temperature stress that is normally used to induce haploid embryogenesis in B. napus. The male gametophyte of Arabidopsis thaliana, which is recalcitrant to haploid embryo development in culture, also forms embryogenic cell clusters after TSA treatment. Genetic analysis suggests that the HDAC protein HDA17 plays a role in this process. TSA treatment of male gametophytes is associated with the hyperacetylation of histones H3 and H4. We propose that the totipotency of the male gametophyte is kept in check by an HDAC-dependent mechanism and that the stress treatments used to induce haploid embryo development in culture impinge on this HDAC-dependent pathway.

وصف الملف: application/octet-stream; application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2df236021591841c61ddaf40c53b427fTest
https://europepmc.org/articles/PMC3963568Test/