The Histone Deacetylase Inhibitor Trichostatin A Promotes Totipotency in the Male Gametophyte[W]
| العنوان: | The Histone Deacetylase Inhibitor Trichostatin A Promotes Totipotency in the Male Gametophyte[W] |
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| المؤلفون: | Jose M. Muiño, Hui Li, Tjitske Riksen, Mercedes Soriano, Jan H.G. Cordewener, Kim Boutilier, Hiroyuki Fukuoka, Gerco C. Angenent |
| المصدر: | The Plant Cell, 26(1), 195-209 The Plant Cell The Plant Cell 26 (2014) 1 |
| بيانات النشر: | American Society of Plant Biologists, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Somatic embryogenesis, medicine.drug_class, Arabidopsis, Plant Science, arabidopsis-thaliana, in-vitro, retinoblastoma protein, Hydroxamic Acids, PRI BIOS Applied Genomics & Proteomics, Histones, BIOS Applied Bioinformatics, Cell Wall, medicine, Arabidopsis thaliana, Laboratorium voor Moleculaire Biologie, brassica-napus l, BIOS Plant Development Systems, plant-cell cycle, Research Articles, Cell Proliferation, Genetics, Gametophyte, biology, EPS-1, Indoleacetic Acids, Arabidopsis Proteins, Histone deacetylase inhibitor, fungi, food and beverages, Embryo, embryo development, Acetylation, Cell Biology, somatic embryogenesis, biology.organism_classification, Cell biology, Histone Deacetylase Inhibitors, Trichostatin A, Histone, biology.protein, microspore embryogenesis, Pollen, Laboratory of Molecular Biology, polycomb-group proteins, Ploidy, pollen embryogenesis, Cell Division, medicine.drug, Signal Transduction |
| الوصف: | The haploid male gametophyte, the pollen grain, is a terminally differentiated structure whose function ends at fertilization. Plant breeding and propagation widely use haploid embryo production from in vitro–cultured male gametophytes, but this technique remains poorly understood at the mechanistic level. Here, we show that histone deacetylases (HDACs) regulate the switch to haploid embryogenesis. Blocking HDAC activity with trichostatin A (TSA) in cultured male gametophytes of Brassica napus leads to a large increase in the proportion of cells that switch from pollen to embryogenic growth. Embryogenic growth is enhanced by, but not dependent on, the high-temperature stress that is normally used to induce haploid embryogenesis in B. napus. The male gametophyte of Arabidopsis thaliana, which is recalcitrant to haploid embryo development in culture, also forms embryogenic cell clusters after TSA treatment. Genetic analysis suggests that the HDAC protein HDA17 plays a role in this process. TSA treatment of male gametophytes is associated with the hyperacetylation of histones H3 and H4. We propose that the totipotency of the male gametophyte is kept in check by an HDAC-dependent mechanism and that the stress treatments used to induce haploid embryo development in culture impinge on this HDAC-dependent pathway. |
| وصف الملف: | application/octet-stream; application/pdf |
| اللغة: | English |
| تدمد: | 1040-4651 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2df236021591841c61ddaf40c53b427fTest https://europepmc.org/articles/PMC3963568Test/ |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2df236021591841c61ddaf40c53b427f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10404651 |
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