التفاصيل البيبلوغرافية
| العنوان: |
Dual dean entrainment with volume ratio modulation for efficient droplet co-encapsulation: extreme single-cell indexing. |
| المؤلفون: |
Harrington, Jack, Esteban, Luis Blay, Butement, Jonathan, Vallejo, Andres F., Lane, Simon I. R., Sheth, Bhavwanti, Jongen, Maaike S. A., Parker, Rachel, Stumpf, Patrick S., Smith, Rosanna C. G., MacArthur, Ben D., Rose-Zerilli, Matthew J. J., Polak, Marta E., Underwood, Tim, West, Jonathan |
| المصدر: |
Lab on a Chip; 9/7/2021, Vol. 21 Issue 17, p3378-3386, 9p |
| مصطلحات موضوعية: |
CELL suspensions, BIOLOGICAL systems, SAMPLE size (Statistics) |
| مستخلص: |
The future of single cell diversity screens involves ever-larger sample sizes, dictating the need for higher throughput methods with low analytical noise to accurately describe the nature of the cellular system. Current approaches are limited by the Poisson statistic, requiring dilute cell suspensions and associated losses in throughput. In this contribution, we apply Dean entrainment to both cell and bead inputs, defining different volume packets to effect efficient co-encapsulation. Volume ratio scaling was explored to identify optimal conditions. This enabled the co-encapsulation of single cells with reporter beads at rates of ~1 million cells per hour, while increasing assay signal-to-noise with cell multiplet rates of ~2.5% and capturing ~70% of cells. The method, called Pirouette coupling, extends our capacity to investigate biological systems. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
