التفاصيل البيبلوغرافية
| العنوان: |
in vitro در محیط CD19 CAR-T و CD19 CAR-CIK مقایسه میزان کشند گی سلول های. (Persian) |
| العنوان البديل: |
In vitro comparison of cytotoxicity of CD19 CAR-CIK and CD19 CAR-T cells. (English) |
| المؤلفون: |
وحید مرادی, اعظم رنجبر, آزاده امیدخدا, ناصر احمدبیگی |
| المصدر: |
Scientific Journal of Iranian Blood Transfusion Organization; Winter2023, Vol. 20 Issue 4, p298-311, 14p |
| مصطلحات موضوعية: |
IN vitro studies, EXPERIMENTAL design, STATISTICS, MONONUCLEAR leukocytes, GRAFT versus host disease, CELL culture, ANALYSIS of variance, CELL receptors, HEALTH outcome assessment, RISK assessment, T-test (Statistics), LACTATE dehydrogenase, DESCRIPTIVE statistics, T cells, CELL separation, DATA analysis, DATA analysis software, ANTIGENS, CELL death, CYTOTOXINS, DISEASE risk factors |
| مستخلص: |
Background and Objectives Despite the success of CAR-T cells in the treatment of hematologic malignancies, the use of allogeneic sources of T lymphocytes to produce these cells is associated with risk of GvHD. Cytokine induced killer (CIK) cells are a population with a lower risk of GvHD. Therefore, CAR-CIK cells can be evaluated as a potential alternative for CAR-T cells. Materials and Methods In this experimental study, after isolation and culture of mononuclear cells isolated from the peripheral blood of two healthy donors, T and CIK cells are transduced with CD19 gene containing lentiviral vector. On the 16th day of culture, to compare the cytotoxicity of CAR-T and CAR-CIK cells, these cells were co-cultured with CD19+ K562 target cells, and the death rate among the target cells was measured using a lactate dehydrogenase release assay kit. Student T-test, Anova and post-hoc Tukey were used for statistical analysis using Graph Pad prism software 8. A p-value less than 0.05 was considered statistically significant. Results In both CAR-CIK and CAR-T cell, CAR receptor caused a significant increase in cytotoxicity. So that no significant difference was observed between the cytotoxicity of CAR-T cells (67.8% ± 1.7) and CAR-CIK (65.1% ± 1.8). Conclusions CAR-CIK cells show a suitable in vitro cytotoxicity, so they can be considered as a potential alternative for CAR-T cells. However, more preclinical and clinical studies are needed to compare other effective dimensions in the function of these two cell types. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
