المؤلفون: Silvia Buonvino, Emilia Di Giovanni, Ivano Amelio, Sonia Melino

المساهمون: Melino, Sonia [0000-0001-7694-5279], Apollo - University of Cambridge Repository

المصدر: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 21, Iss 5, p 1638 (2020)
Volume 21
Issue 5

مصطلحات موضوعية: cell migration, Transcription, Genetic, hydrogen sulfide, garlic, regenerative medicine, oxidative stress, MSCs, organosulfur compounds, cell differentiation, Cellular differentiation, medicine.medical_treatment, Cell Plasticity, Regenerative medicine, Antioxidants, lcsh:Chemistry, Cell Movement, lcsh:QH301-705.5, Spectroscopy, Cell Differentiation, General Medicine, Stem-cell therapy, Glutathione, Cell Hypoxia, Computer Science Applications, Phenotype, Cardiovascular Diseases, Inactivation, Metabolic, Stem cell, Cell Survival, Mesenchymal Stem Cell Transplantation, Catalysis, Article, Inorganic Chemistry, ddc:570, medicine, Humans, Settore BIO/10, Physical and Theoretical Chemistry, Molecular Biology, Cell Proliferation, Wound Healing, Sulfur Compounds, Cell growth, business.industry, Myocardium, Organic Chemistry, Mesenchymal stem cell, Mesenchymal Stem Cells, Fibroblasts, equipment and supplies, Transplantation, lcsh:Biology (General), lcsh:QD1-999, Gene Expression Regulation, Cancer research, business, Biomarkers, Homing (hematopoietic)

الوصف: The endogenous gasotransmitter H2S plays an important role in the central nervous, respiratory and cardiovascular systems. Accordingly, slow-releasing H2S donors are powerful tools for basic studies and innovative pharmaco-therapeutic agents for cardiovascular and neurodegenerative diseases. Nonetheless, the effects of H2S-releasing agents on the growth of stem cells have not been fully investigated. H2S preconditioning can enhance mesenchymal stem cell survival after post-ischaemic myocardial implantation
therefore, stem cell therapy combined with H2S may be relevant in cell-based therapy for regenerative medicine. Here, we studied the effects of slow-releasing H2S agents on the cell growth and differentiation of cardiac Lin&minus
Sca1+ human mesenchymal stem cells (cMSC) and on normal human dermal fibroblasts (NHDF). In particular, we investigated the effects of water-soluble GSH&ndash
garlic conjugates (GSGa) on cMSC compared to other H2S-releasing agents, such as Na2S and GYY4137. GSGa treatment of cMSC and NHDF increased their cell proliferation and migration in a concentration dependent manner with respect to the control. GSGa treatment promoted an upregulation of the expression of proteins involved in oxidative stress protection, cell&ndash
cell adhesion and commitment to differentiation. These results highlight the effects of H2S-natural donors as biochemical factors that promote MSC homing, increasing their safety profile and efficacy after transplantation, and the value of these donors in developing functional 3D-stem cell delivery systems for cardiac muscle tissue repair and regeneration.

وصف الملف: application/pdf; application/zip; text/xml

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9faf48cde51aebcedc83c1f87cca5dbTest

المؤلفون: Emilia Di Giovanni, Sonia Melino, Ilaria Cacciotti, Matteo Ciocci, Francesca Nanni

المصدر: International Journal of Molecular Sciences, Vol 19, Iss 8, p 2368 (2018)
International Journal of Molecular Sciences
Volume 19
Issue 8

مصطلحات موضوعية: Male, 0301 basic medicine, 02 engineering and technology, Regenerative medicine, lcsh:Chemistry, Hydrogen Sulfide, lcsh:QH301-705.5, Spectroscopy, Aged, 80 and over, Chemistry, Biomaterial, General Medicine, Middle Aged, 021001 nanoscience & nanotechnology, Computer Science Applications, Membrane, PLA fibers, antibacterial properties, cytotoxicity, garlic extracts, mesenchymal stem cells, microstructure, organosulfur compounds, thermal and mechanical properties, Female, Stem cell, 0210 nano-technology, Cell signaling, Cell Survival, Settore ING-IND/22 - Scienza e Tecnologia dei Materiali, Context (language use), Article, Catalysis, Cell Line, Inorganic Chemistry, 03 medical and health sciences, Humans, Settore BIO/10, Physical and Theoretical Chemistry, Garlic, Molecular Biology, Aged, Cell Proliferation, Plant Extracts, Organic Chemistry, Mesenchymal stem cell, Membranes, Artificial, In vitro, Oxidative Stress, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, Biophysics

الوصف: The design of biomaterial platforms able to release bioactive molecules is mandatory in tissue repair and regenerative medicine. In this context, electrospinning is a user-friendly, versatile and low-cost technique, able to process different kinds of materials in micro- and nano-fibers with a large surface area-to-volume ratio for an optimal release of gaseous signaling molecules. Recently, the antioxidant and anti-inflammatory properties of the endogenous gasotramsmitter hydrogen sulfide (H2S), as well as its ability to stimulate relevant biochemical processes on the growth of mesenchymal stem cells (MSC), have been investigated. Therefore, in this work, new poly(lactic) acid fibrous membranes (PFM), doped and functionalized with H2S slow-releasing donors extracted from garlic, were synthetized. These innovative H2S-releasing mats were characterized for their morphological, thermal, mechanical, and biological properties. Their antimicrobial activity and effects on the in vitro human cardiac MSC growth, either in the presence or in the absence of oxidative stress, were here assessed. On the basis of the results here presented, these new H2S-releasing PFM could represent promising and low-cost scaffolds or patches for biomedical applications in tissue repair.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24ed6df63f8afe0086c39cdf1348ffe7Test
http://www.mdpi.com/1422-0067/19/8/2368Test