Cyclin-Dependent Kinase Inhibitors KRP1 and KRP2 Are Involved in Grain Filling and Seed Germination in Rice (Oryza sativa L.)
| العنوان: | Cyclin-Dependent Kinase Inhibitors KRP1 and KRP2 Are Involved in Grain Filling and Seed Germination in Rice (Oryza sativa L.) |
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| المؤلفون: | Wanning Liu, Liqun Tang, Xixi Liu, Shuang Wang, Xiaohong Tong, Jian Zhang, Shufan Li, Juan Zhao, Abolore Adijat Ajadi, Yifeng Wang, Yazhou Shu, Zhiyong Li, Huimei Wang, Sani Muhammad Tajo |
| المصدر: | International Journal of Molecular Sciences, Vol 21, Iss 1, p 245 (2019) International Journal of Molecular Sciences Volume 21 Issue 1 |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, grain filling, Cell Cycle Proteins, 01 natural sciences, lcsh:Chemistry, chemistry.chemical_compound, cyclin-dependent kinase inhibitors, Gene Expression Regulation, Plant, Brassinosteroid, Abscisic acid, lcsh:QH301-705.5, Spectroscopy, Cyclin-Dependent Kinase Inhibitor Proteins, Plant Proteins, biology, Protoplasts, Gene Expression Regulation, Developmental, food and beverages, General Medicine, Cell cycle, Cyclin-Dependent Kinases, Computer Science Applications, Cell biology, Germination, Seeds, seed germination, rice (oryza sativa l.), Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, Brassinosteroids, Endoreduplication, Physical and Theoretical Chemistry, Molecular Biology, Cell Proliferation, Cell Nucleus, Oryza sativa, Arabidopsis Proteins, Cell growth, Organic Chemistry, Oryza, biology.organism_classification, 030104 developmental biology, chemistry, lcsh:Biology (General), lcsh:QD1-999, Seedling, Mutation, rice (Oryza sativa L.), Edible Grain, Abscisic Acid, 010606 plant biology & botany |
| الوصف: | Cyclin-dependent kinase inhibitors known as KRPs (kip-related proteins) control the progression of plant cell cycles and modulate various plant developmental processes. However, the function of KRPs in rice remains largely unknown. In this study, two rice KRPs members, KRP1 and KRP2, were found to be predominantly expressed in developing seeds and were significantly induced by exogenous abscisic acid (ABA) and Brassinosteroid (BR) applications. Sub-cellular localization experiments showed that KRP1 was mainly localized in the nucleus of rice protoplasts. KRP1 overexpression transgenic lines (OxKRP1), krp2 single mutant (crkrp2), and krp1/krp2 double mutant (crkrp1/krp2) all exhibited significantly smaller seed width, seed length, and reduced grain weight, with impaired seed germination and retarded early seedling growth, suggesting that disturbing the normal steady state of KRP1 or KRP2 blocks seed development partly through inhibiting cell proliferation and enlargement during grain filling and seed germination. Furthermore, two cyclin-dependent protein kinases, CDKC 2 and CDKF 3, could interact with KRP1 in a yeast-two-hybrid system, indicating that KRP1 might regulate the mitosis cell cycle and endoreduplication through the two targets. In a word, this study shed novel insights into the regulatory roles of KRPs in rice seed maturation and germination. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0659e0f1c5f0182a0835e8d27201ac20Test https://www.mdpi.com/1422-0067/21/1/245Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0659e0f1c5f0182a0835e8d27201ac20 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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