التفاصيل البيبلوغرافية
| العنوان: |
Galectin-3 alters the lateral mobility and clustering of β1-integrin receptors. |
| المؤلفون: |
Yang, Esther H., Rode, Julia, Howlader, Md. Amran, Eckermann, Marina, Santos, Jobette T., Hernandez Armada, Daniel, Zheng, Ruixiang, Zou, Chunxia, Cairo, Christopher W. |
| المصدر: |
PLoS ONE; 10/10/2017, Vol. 12 Issue 10, p1-17, 17p |
| مصطلحات موضوعية: |
GLYCOPROTEIN receptors, GALECTINS, INTEGRINS, CELL migration, FLUORESCENCE microscopy |
| مستخلص: |
Glycoprotein receptors are influenced by myriad intermolecular interactions at the cell surface. Specific glycan structures may interact with endogenous lectins that enforce or disrupt receptor-receptor interactions. Glycoproteins bound by multivalent lectins may form extended oligomers or lattices, altering the lateral mobility of the receptor and influencing its function through endocytosis or changes in activation. In this study, we have examined the interaction of Galectin-3 (Gal-3), a human lectin, with adhesion receptors. We measured the effect of recombinant Gal-3 added exogenously on the lateral mobility of the α5β1 integrin on HeLa cells. Using single-particle tracking (SPT) we detected increased lateral mobility of the integrin in the presence of Gal-3, while its truncated C-terminal domain (Gal-3C) showed only minor reductions in lateral mobility. Treatment of cells with Gal-3 increased β1-integrin mediated migration with no apparent changes in viability. In contrast, Gal-3C decreased both cell migration and viability. Fluorescence microscopy allowed us to confirm that exogenous Gal-3 resulted in reorganization of the integrin into larger clusters. We used a proteomics analysis to confirm that cells expressed endogenous Gal-3, and found that addition of competitive oligosaccharide ligands for the lectin altered the lateral mobility of the integrin. Together, our results are consistent with a Gal-3–integrin lattice model of binding and confirm that the lateral mobility of integrins is natively regulated, in part, by galectins. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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