A simplified strategy to package foreign proteins into baculovirus occlusion bodies without engineering the viral genome
| العنوان: | A simplified strategy to package foreign proteins into baculovirus occlusion bodies without engineering the viral genome |
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| المؤلفون: | Tomás Masson, María Laura Fabre, Víctor Romanowski, Santiago Haase, María Leticia Ferrelli |
| المصدر: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Proteomics, food.ingredient, Insecta, ACMNPV, viruses, Biología, Heterologous, Bioengineering, Occlusion Bodies, Viral, 01 natural sciences, Applied Microbiology and Biotechnology, Green fluorescent protein, Cell Line, purl.org/becyt/ford/1 [https], 03 medical and health sciences, chemistry.chemical_compound, food, Polyhedron envelope protein (PEP), Genes, Reporter, 010608 biotechnology, AGMNPV, UFLAg-286, Animals, purl.org/becyt/ford/1.6 [https], POLYHEDRON ENVELOPE PROTEIN (PEP), biology, fungi, Wild type, AgMNPV, General Medicine, AcMNPV, biology.organism_classification, Fusion protein, Occlusion Body Matrix Proteins, Nucleopolyhedroviruses, Recombinant Proteins, Cell biology, Genetically modified organism, Alphabaculovirus, Autographa californica, 030104 developmental biology, chemistry, UFLAG-286, Baculoviridae, DNA, Biotechnology |
| الوصف: | Polyhedron envelope protein (PEP) is the major component of the calyx that surrounds the baculovirus occlusion body (OB). PEP has been associated with the stabilization and resistance of polyhedra in the environment. Due to the abundant levels of PEP in OBs, we decided to use this protein as a fusion partner to redirect foreign proteins to baculovirus polyhedra. In this study we developed a strategy that involves the generation of a monoclonal transformed insect cell line expressing a protein of interest fused to the the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) N-terminus of PEP that enables the packaging of foreign proteins into the OBs without generating a recombinant baculovirus. This proved to be an efficient platform that could be exploited to improve wild type baculovirus for their use as bioinsecticides without facing the concerns of releasing genetically modified DNA to the environment and bypassing the associated regulatory issues. We demonstrated, using immunological, proteomic and microscopy techniques, that the envelope of AgMNPV OBs can effectively trap chimeric proteins in an infected insect cell line expressing AgMNPV PEP fused to the enhanced green fluorescent protein (eGFP). Furthermore, packaging of chimeric PEP also took place with heterologous OBs such as those of Autographa californica multiple nucleopolyhedrovirus (AcMNPV), another group I alphabaculovirus. Instituto de Biotecnologia y Biologia Molecular |
| وصف الملف: | application/pdf |
| تدمد: | 1873-4863 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9966dff9aa9cc19d7527f58d78ae0214Test https://pubmed.ncbi.nlm.nih.gov/31715205Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9966dff9aa9cc19d7527f58d78ae0214 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18734863 |
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