Genetic evidence that mycobacterial FtsZ and FtsW proteins interact, and colocalize to the division site inMycobacterium smegmatis
| العنوان: | Genetic evidence that mycobacterial FtsZ and FtsW proteins interact, and colocalize to the division site inMycobacterium smegmatis |
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| المؤلفون: | Malini Rajagopalan, Hava Lofton, Jarosław Dziadek, Erin Maloney, Murty V. V. S. Madiraju, Ashwini Chauhan, Marta Popławska |
| المصدر: | FEMS Microbiology Letters. 250:9-17 |
| بيانات النشر: | Oxford University Press (OUP), 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Cell division, Recombinant Fusion Proteins, Mycobacterium smegmatis, Biological Transport, Active, macromolecular substances, medicine.disease_cause, physiological processes, Microbiology, Plasmid, Bacterial Proteins, Two-Hybrid System Techniques, Escherichia coli, Genetics, medicine, FtsZ, Molecular Biology, Gene, Aspartic Acid, Base Sequence, biology, Membrane Proteins, Colocalization, biology.organism_classification, Cell biology, Cytoskeletal Proteins, Genes, Bacterial, biology.protein, bacteria, biological phenomena, cell phenomena, and immunity, Cell Division, Plasmids, Mycobacterium |
| الوصف: | We provide genetic evidence to show that the Mycobacterium tuberculosis FtsZ and FtsW proteins interact, and that these interactions are biologically relevant. Furthermore, we show by fluorescence microscopy that Mycobacterium smegmatis FtsW is part of its septasomal complex and colocalizes with FtsZ to the midcell sites. Colocalization experiments reveal that approximately 27% of the cells with septal Z-rings contain FtsW whereas 93% of the cells with FtsW bands are associated with FtsZ indicating that FtsW is late recruit to the septum, as in Escherichia coli. Our results suggest that mycobacterial FtsZ can localize to the septum independent of FtsW, and that interactions of FtsW with FtsZ are critical for the formation of productive FtsZ-rings and the cell division process in mycobacteria. |
| تدمد: | 1574-6968 0378-1097 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::edff8853475169822f6ac29953a40c5dTest https://doi.org/10.1016/j.femsle.2005.06.043Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....edff8853475169822f6ac29953a40c5d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15746968 03781097 |
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