التفاصيل البيبلوغرافية
| العنوان: |
Central role for TCR/CD3 ligation in the differentiation of CD4+ T cells toward A Th1 or Th2 functional phenotype |
| المؤلفون: |
Roecken, Martin, Mueller, Kai Michael, Saurat, Jean-Hilaire, Mueller, Ingrid Ursula, Louis, Jacques A., Cerottini, Jean-Charles, Hauser, Conrad |
| المصدر: |
ISSN: 0022-1767 ; Journal of Immunology, vol. 148, no. 1 (1992) p. 47-54. |
| سنة النشر: |
1992 |
| المجموعة: |
Université de Genève: Archive ouverte UNIGE |
| مصطلحات موضوعية: |
info:eu-repo/classification/ddc/610, Animals, Antigens, Differentiation, T-Lymphocyte, Physiology, Immunology, B-Lymphocytes, CD3 Complex, CD4-Positive T-Lymphocytes, Cytology, Cell Differentiation, Enterotoxins, Female, Immunologic Memory, Interferon-gamma, Interleukin-2, Metabolism, Pharmacology, Interleukin-4, Lymphocyte Activation, Lymphokines, Biosynthesis, Mice, Receptors, Antigen, T-Cell, Helper-Inducer, Thy-1 Antigens |
| الوصف: |
Activated CD4+ T cells can be classified into distinct subsets; the most divergent among them may be considered to be the IL-2 and IFN-gamma-producing Th1 clones and the IL-4 and IL-5-producing Th2 clones. Because Th1 and Th2 clones can usually be detected only after several months of culture, we used conditions that modulate the IL-2 and IL-4 production in short term culture. Here we show that freshly isolated and subsequently in vitro-activated CD4+ T cells that were cultured for 11 days with rIL-2 and restimulated showed a IFN-gamma+ IL-2+ IL-3+ IL-4- IL-5- pattern. Because these cells were not capable of providing B cell help for IgG1, IgG2a, or IgE in an APC- and TCR-dependent T-B cell assay, they expressed a phenotype typical for most Th1 clones. In contrast, activated T cells that were cultured for 11 days with IL-2 plus a mAb to CD3 and then restimulated produced a IFN-gamma- IL-2- IL-3+ IL-4+ IL-5+ pattern. These cells were capable of providing B cell help for IgG1, IgG2a, and IgE synthesis and thus presented a phenotype typical for Th2 clones. Similar results were observed when mitogenic mAb to Thy-1.2 or to framework determinants of the alpha beta TCR were used. The induction of Th1- and Th2-like cells did not depend on the relative expression of CD44 or CD45 by the T cells before activation in vitro. Because the incubation of activated T cells with anti-CD3/TCR mAb induced high unrestricted lymphokine production, the latter might be responsible for the Th2-like lymphokine pattern observed after restimulation. To address this point, TCR V beta 8+ and V beta 8- T cell blasts were co-cultured in the presence of mAb to V beta 8. After restimulation, V beta 8+ cells had a IL-4high IL-2low phenotype and V beta 8- cells had a IL-4low IL-2high phenotype. This demonstrates that TCR ligation but not lymphokines alone are capable of inducing Th2-like cells, and this points out a central role for the TCR in the generation of T cell subsets. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| العلاقة: |
info:eu-repo/semantics/altIdentifier/pmid/1345789; https://archive-ouverte.unige.ch/unige:106687Test; unige:106687 |
| الإتاحة: |
https://archive-ouverte.unige.ch/unige:106687Test |
| حقوق: |
info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: |
edsbas.BD7B195C |
| قاعدة البيانات: |
BASE |
