دورية أكاديمية
Analysis of Exosomal Cargo Provides Accurate Clinical, Histologic and Mutational Information in Non-Small Cell Lung Cancer
| العنوان: | Analysis of Exosomal Cargo Provides Accurate Clinical, Histologic and Mutational Information in Non-Small Cell Lung Cancer |
|---|---|
| المؤلفون: | Duréndez-Sáez, Elena, Calabuig-Fariñas, Silvia, Torres-Martínez, Susana, Moreno-Manuel, Andrea, Herreros-Pomares, Alejandro, Escorihuela, Eva, Mosqueda, Marais, Gallach, Sandra, Guijarro, Ricardo, Serna, Eva, Suárez-Cabrera, Cristian, Paramio, Jesús M., Blasco, Ana, Camps, Carlos, Jantus-Lewintre, Eloisa |
| المساهمون: | Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials, Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural, European Commission, Generalitat Valenciana, Instituto de Salud Carlos III, Asociación Española Contra el Cáncer, Centro de Investigación Biomédica en Red de Cáncer |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2022 |
| المجموعة: | Universitat Politécnica de Valencia: RiuNet / Politechnical University of Valencia |
| مصطلحات موضوعية: | Non-small cell lung cancer, Liquid biopsy, Exosomes, Extracellular vesicles, Cell cultures, Adenocarcinoma, Squamous cell carcinoma, Biomarker, Tumorspheres, BIOLOGIA CELULAR |
| الوصف: | [EN] Lung cancer is a malignant disease with high mortality and poor prognosis, frequently diagnosed at advanced stages. Nowadays, immense progress in treatment has been achieved. However, the present scenario continues to be critical, and a full comprehension of tumor progression mechanisms is required, with exosomes being potentially relevant players. Exosomes are membranous vesicles that contain biological information, which can be transported cell-to-cell and modulate relevant processes in the hallmarks of cancer. The present research aims to characterize the exosomes' cargo and study their role in NSCLC to identify biomarkers. We analyzed exosomes secreted by primary cultures and cell lines, grown in monolayer and tumorsphere formations. Exosomal DNA content showed molecular alterations, whereas RNA high-throughput analysis resulted in a pattern of differentially expressed genes depending on histology. The most significant differences were found in XAGE1B, CABYR, NKX2-1, SEPP1, CAPRIN1, and RIOK3 genes when samples from two independent cohorts of resected NSCLC patients were analyzed. We identified and validated biomarkers for adenocarcinoma and squamous cell carcinoma. Our results could represent a relevant contribution concerning exosomes in clinical practice, allowing for the identification of biomarkers that provide information regarding tumor features, prognosis and clinical behavior of the disease. ; This research was founded by Centro de Investigacion Biomedica en Red de Cancer (CIBERONC) (grant number CB16-12-00350), Instituto de Salud Carlos III (PI18/00266), and Generalitat Valenciana (GV/2018/026). The authors E.D.-S. and A.M.-M. have a predoctoral fellowship (PRDVA18015MORE) from the Scientific Foundation of Asociación Española Contra el Cáncer, Valencia (AECC Valencia). A.H.-P. is supported by Ayudas Margarita Salas from Ministerio de Universidades, Unión EuropeaNext generation EU. S.T.-M. is supported by the Generalitat Valenciana and Fondo Social Europeo fellowship (ACIF/2018/275). ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2072-6694 |
| العلاقة: | Cancers; info:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 (ISCIII)/PI18%2F00266/ES/INMUNOGRAMA NO INVASIVO. APROXIMACION MULTIDIMENSIONAL PARA CARACTERIZAR Y MONITORIZAR EL ESTATUS INMUNE EN CANCER DE PULMON./; info:eu-repo/grantAgreement/GVA//GV%2F2018%2F026/; info:eu-repo/grantAgreement/GVA//ACIF%2F2018%2F275/; info:eu-repo/grantAgreement/AECC//PRDVA18015MORE/; info:eu-repo/grantAgreement/CIBERONC//CB16-12-00350/; https://doi.org/10.3390/cancers14133216Test; http://hdl.handle.net/10251/203855Test; urn:eissn:2072-6694; PMC9264915 |
| DOI: | 10.3390/cancers14133216 |
| الإتاحة: | https://doi.org/10.3390/cancers14133216Test http://hdl.handle.net/10251/203855Test |
| حقوق: | http://creativecommons.org/licenses/by/4.0Test/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.354464BC |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20726694 |
|---|---|
| DOI: | 10.3390/cancers14133216 |