التفاصيل البيبلوغرافية
| العنوان: |
True |
| المؤلفون: |
Sara Vignati, Jose Jimeno, I Muradore, S Ronzoni, Eugenio Erba, Maurizio D'Incalci, Daniele Bergamaschi, G Di Liberti, Glynn Faircloth, L Bassano |
| المصدر: |
British Journal of Cancer. 82:1732-1739 |
| بيانات النشر: |
Springer Science and Business Media LLC, 2000. |
| سنة النشر: |
2000 |
| مصطلحات موضوعية: |
Cancer Research, medicine.medical_specialty, medicine.diagnostic_test, Clone (cell biology), Drug resistance, Biology, Ecteinascidia turbinata, biology.organism_classification, Flow cytometry, Endocrinology, Oncology, Cell culture, Internal medicine, medicine, Cancer research, biology.protein, Doxorubicin, Efflux, medicine.drug, P-glycoprotein |
| الوصف: |
By exposing Igrov-1 human ovarian cancer cells to increasing concentrations of Ecteinascidin-743 (ET-743), either for a short or prolonged time, we obtained sublines resistant to ET-743 which overexpress Pgp. The most resistant clone (Igrov-1/25 ET) was evaluated for biological and pharmacological characterizations. The increased Pgp levels of Igrov-1/25 ET were not due to amplification of the mdr-1 gene but to increased mRNA levels. No increase in other multidrug resistance-related proteins such as MRP or LRP was observed in Igrov-1/25 ET. The IC50values of ET-743 against Igrov-1/25 ET was approximately 50 times higher than the parental cell line. Resistance was not reversed while maintaining the cell line in drug-free medium for at least 24 months. Igrov-1/25 ET was cross-resistant to Doxorubicin and VP16 while it was equally sensitive to L-PAM, MNNG, CPT and only marginally less sensitive to Cis-DDP and Oxaliplatin compared to the parental cell line. Igrov-1/25 ET exposed to Doxorubicin retained this drug much less, mainly because of a more efficient drug efflux. The cyclosporine analogue SDZ PSC-833 reversed the resistance of Igrov-1/25 ET to ET-743, without any enhancement of the drug activity against the parental Igrov-1 cell line. Igrov-1/25 ET exhibits typical features of cell lines overexpressing the mdr-1 gene and can be a potentially useful tool in selecting ET-743 non-cross-resistant analogues as well as to investigate methods to counteract resistance to this drug. © 2000 Cancer Research Campaign |
| تدمد: |
1532-1827 |
| الوصول الحر: |
https://explore.openaire.eu/search/publication?articleId=doi_________::e98f98f6c0949cbb60670c325b395042Test
https://doi.org/10.1054/bjoc.2000.1224Test |
| حقوق: |
OPEN |
| رقم الانضمام: |
edsair.doi...........e98f98f6c0949cbb60670c325b395042 |
| قاعدة البيانات: |
OpenAIRE |
