Ninein is released from the centrosome and moves bi-directionally along microtubules
| العنوان: | Ninein is released from the centrosome and moves bi-directionally along microtubules |
|---|---|
| المؤلفون: | David K. Moss, Mette M. Mogensen, Mirjana Liovic, E. Birgitte Lane, Jennifer Keynton, Gemma Bellett, Jane M. Carter, Alan R. Prescott |
| المصدر: | Journal of Cell Science. 120:3064-3074 |
| بيانات النشر: | The Company of Biologists, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Cytoplasm, Recombinant Fusion Proteins, Biology, Microtubules, Cell Line, Adherens junction, Glycogen Synthase Kinase 3, Mice, Epithelial Differentiation, Microtubule, Animals, Humans, Microtubule anchoring, Centrosome, Glycogen Synthase Kinase 3 beta, Nocodazole, Nuclear Proteins, Fluorescence recovery after photobleaching, Cell Differentiation, Epithelial Cells, Cell Biology, Bridged Bicyclo Compounds, Heterocyclic, Tubulin Modulators, Cell biology, Cytoskeletal Proteins, Cell culture, Ear, Inner, Thiazolidines, Fluorescence Recovery After Photobleaching |
| الوصف: | Cell-to-cell contact and polarisation of epithelial cells involve a major reorganisation of the microtubules and centrosomal components. The radial microtubule organisation is lost and an apico-basal array develops that is no longer anchored at the centrosome. This involves not only the relocation of microtubules but also of centrosomal anchoring proteins to apical non-centrosomal sites. The relocation of microtubule minus-end-anchoring proteins such as ninein to the apical sites is likely to be essential for the assembly and stabilisation of the apico-basal arrays in polarised epithelial cells. In this study, we establish that ninein is highly dynamic and that, in epithelial cells, it is present not only at the centrosome but also in the cytoplasm as distinct speckles. Live-cell imaging reveals that GFP-ninein speckles are released from the centrosome and move in a microtubule-dependent manner within the cytoplasm and thus establishes that epithelial cells possess the mechanical means for relocation of ninein to non-centrosomal anchoring sites. We also provide evidence for the deployment of ninein speckles to apical anchoring sites during epithelial differentiation in both an in situ tissue and an in vitro culture system. In addition, the findings suggest that the non-centrosomal microtubule anchoring sites associate with adherens junctions in polarised epithelial cells. |
| تدمد: | 1477-9137 0021-9533 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2070b3db43f0e1b6d2dfc858305499ddTest https://doi.org/10.1242/jcs.010322Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2070b3db43f0e1b6d2dfc858305499dd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779137 00219533 |
|---|