التفاصيل البيبلوغرافية
| العنوان: |
High level expression, purification, and in vivo activity of bovine granulocyte-colony stimulating factor produced using a baculovirus system |
| المؤلفون: |
Nagata, Tomoshi1, Tohda, Yukihiko1, Yokomizo, Yuichi2, Nakamura, Masayuki1, Takehara, Kazuaki1 takehara@vmas.kitasato-u.ac.jp |
| المصدر: |
Veterinary Immunology & Immunopathology. Nov2003, Vol. 96 Issue 1/2, p105. 6p. |
| مصطلحات موضوعية: |
*GRANULOCYTE-macrophage colony-stimulating factor, *GENE expression, *BACULOVIRUS genetics, *CELL culture |
| مستخلص: |
A bovine granulocyte-colony stimulating factor (bG-CSF) cDNA clone bearing a C-terminal poly-His-tag (bG-CSFHis) was constructed and expressed by the baculovirus expression system. The bG-CSFHis was expressed as an approximately 19 kDa protein in the culture supernatants and was purified using a nickel chelate column. The purified bG-CSFHis had bioactivity in vitro in the NFS-60 bioassay. In order to evaluate activity in vivo, purified bG-CSFHis was administered to cattle as single or multiple dosages. The bG-CSFHis increased neutrophil counts in peripheral blood and modulated the phagocytic activity of the neutrophils. The data indicates that the recombinant protein had activity in vivo. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
Academic Search Index |
