المؤلفون: Yam B. Poudel, Liqi He, Matthew Cox, Qian Zhang, Walter L. Johnson, Qiang Cong, Heng Cheng, Naidu S. Chowdari, Christine Tarby, Andrew F. Donnell, Matthais Broekema, Daniel P. O’Malley, Yong Zhang, Murugaiah A. M. Subbaiah, Boda Vijay Kumar, Lakshumanan Subramani, Bei Wang, Yi-Xin Li, Prasanna Sivaprakasam, David Critton, Dawn Mulligan, Bhupindar Sandhu, Chunshan Xie, Radha Ramakrishnan, Jignesh Nagar, Shailesh Dudhgaonkar, Martins S. Oderinde, Anwar Murtaza, Gary L. Schieven, Arvind Mathur, Ashvinikumar V. Gavai, Gregory Vite, Sanjeev Gangwar

مصطلحات موضوعية: Biochemistry, Medicine, Cell Biology, Genetics, Pharmacology, Biotechnology, Immunology, Cancer, Computational Biology, Chemical Sciences not elsewhere classified, mouse whole blood, complete tumor regression, 26 tumor model, based reporter assay, selective tlr7 agonists, novel tlr7 agonists, tlr7 agonists, developed novel, based designs, vitro <, tnfα cytokines, systemic agent, significant secretion, pharmacodynamic studies, mice showed, intravenous route

الوصف: We have designed and developed novel and selective TLR7 agonists that exhibited potent receptor activity in a cell-based reporter assay. In vitro , these agonists significantly induced secretion of cytokines IL-6, IL-1β, IL-10, TNFa, IFNa, and IP-10 in human and mouse whole blood. Pharmacokinetic and pharmacodynamic studies in mice showed a significant secretion of IFNα and TNFα cytokines. When combined with aPD1 in a CT-26 tumor model, the lead compound showed strong synergistic antitumor activity with complete tumor regression in 8/10 mice dosed using the intravenous route. Structure–activity relationship studies enabled by structure-based designs of TLR7 agonists are disclosed.

العلاقة: https://figshare.com/articles/journal_contribution/Discovery_of_Novel_TLR7_Agonists_as_Systemic_Agent_for_Combination_With_aPD1_for_Use_in_Immuno-oncology/24961448Test

الإتاحة: https://doi.org/10.1021/acsmedchemlett.3c00455.s001Test
https://figshare.com/articles/journal_contribution/Discovery_of_Novel_TLR7_Agonists_as_Systemic_Agent_for_Combination_With_aPD1_for_Use_in_Immuno-oncology/24961448Test

المؤلفون: Ramakrishnan, Chandra, Fort, Cécile, Marques, Sara Rute, Ferguson, David J P, Gransagne, Marion, Baum, Jake, Chaouch, Soraya, Mouray, Elisabeth, Kohl, Linda, Wheeler, Richard J, Sinden, Robert E

المصدر: Ramakrishnan, Chandra; Fort, Cécile; Marques, Sara Rute; Ferguson, David J P; Gransagne, Marion; Baum, Jake; Chaouch, Soraya; Mouray, Elisabeth; Kohl, Linda; Wheeler, Richard J; Sinden, Robert E (2023). Radial spoke protein 9 is necessary for axoneme assembly in Plasmodium but not in trypanosomatid parasites. Journal of Cell Science, 136(11):jcs260655.

مصطلحات موضوعية: Institute of Parasitology, 610 Medicine & health, 600 Technology, 570 Life sciences, biology, Cell Biology

الوصف: Flagella are important for eukaryote cell motility, including in sperm, and are vital for life cycle progression of many unicellular eukaryotic pathogens. The ‘9+2’ axoneme in most motile flagella comprises nine outer doublet and two central-pair singlet microtubules. T-shaped radial spokes protrude from the outer doublets towards the central pair and are necessary for effective beating. We asked whether there were radial spoke adaptations associated with parasite lineage-specific properties in apicomplexans and trypanosomatids. Following an orthologue search for experimentally uncharacterised radial spoke proteins (RSPs), we identified and analysed RSP9. Trypanosoma brucei and Leishmania mexicana have an extensive RSP complement, including two divergent RSP9 orthologues, necessary for flagellar beating and swimming. Detailed structural analysis showed that neither orthologue is needed for axoneme assembly in Leishmania. In contrast, Plasmodium has a reduced set of RSPs including a single RSP9 orthologue, deletion of which in Plasmodium berghei leads to failure of axoneme formation, failed male gamete release, greatly reduced fertilisation and inefficient life cycle progression in the mosquito. This indicates contrasting selection pressures on axoneme complexity, likely linked to the different mode of assembly of trypanosomatid versus Plasmodium flagella.

وصف الملف: application/pdf

العلاقة: https://www.zora.uzh.ch/id/eprint/255353/1/Radial_spoke_protein_9_is_necessary.pdfTest; info:pmid/37288670; urn:issn:0021-9533

الإتاحة: https://doi.org/10.5167/uzh-25535310.1242/jcs.260655Test
https://www.zora.uzh.ch/id/eprint/255353Test/
https://www.zora.uzh.ch/id/eprint/255353/1/Radial_spoke_protein_9_is_necessary.pdfTest

المؤلفون: Sarena Banu (14341577), Ramakrishnan Nagaraj (448256), Mohammed M. Idris (14341580)

مصطلحات موضوعية: Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Immunology, Mental Health, Infectious Diseases, Virology, Computational Biology, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, several clinical observations, inflammatory response pathways, +infection%22">xlink "> infection, detailed proteomic analysis, immune cell trafficking, cell signaling interaction, initial infection region, 2 virus results, found differentially regulated, 2 infection, proteomic perspective, oropharyngeal region, differentially regulated, tested positive, serious effects, organ failure, normal individuals, infected individuals

الوصف: (DOCX)

العلاقة: https://figshare.com/articles/journal_contribution/Acquisition_parameters_used_for_LC-MS_MS_analysis_/21832985Test

الإتاحة: https://doi.org/10.1371/journal.pone.0279998.s001Test

المؤلفون: Martin, Carrie, Meadors, Sara, Malik, Shahbaz, Collins, Keri, Velasquez, Tomeka, Brooks, Kathryn Ashleigh, Scofield, Danielle, Clemmer, Kelly, Stentz, Jacklyn, Rana, Cydney, Burton, Mary Jo, Ramakrishnan, Dr. Aravind, Shaughnessy, Paul J.

المصدر: Transplantation and Cellular Therapy ; volume 29, issue 2, page S352 ; ISSN 2666-6367

مصطلحات موضوعية: Transplantation, Cell Biology, Hematology, Molecular Medicine, Immunology and Allergy

الإتاحة: https://doi.org/10.1016/s2666-6367Test(23)00534-1
https://api.elsevier.com/content/article/PII:S2666636723005341?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2666636723005341?httpAccept=text/plainTest

المؤلفون: Lue, Brian Ko-Hung, Patel, Akshat Maneesh, Reves, Heather, Afrough, Aimaz, Anderson, Larry D., Chung, Stephen, Collins, Robert H., Kaur, Gurbakhash, Kumar, Kiran, Madanat, Yazan F., Vusirikala, Madhuri, Awan, Farrukh T, Tessier, Jeffrey M, Geethakumari, Praveen Ramakrishnan

المصدر: Transplantation and Cellular Therapy ; volume 29, issue 2, page S208-S209 ; ISSN 2666-6367

مصطلحات موضوعية: Transplantation, Cell Biology, Hematology, Molecular Medicine, Immunology and Allergy

الإتاحة: https://doi.org/10.1016/s2666-6367Test(23)00339-1
https://api.elsevier.com/content/article/PII:S2666636723003391?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2666636723003391?httpAccept=text/plainTest

المؤلفون: Shea, Lauren K., Akhave, Neal S., Sutton, Leslie A., Compton, Leigh A., York, Conner, Ramakrishnan, Sai Mukund, Miller, Christopher A., Wartman, Lukas D., Chen, David Y.

المصدر: Journal of Investigative Dermatology ; volume 143, issue 2, page 232-241.e6 ; ISSN 0022-202X

مصطلحات موضوعية: Cell Biology, Dermatology, Molecular Biology, Biochemistry

الإتاحة: https://doi.org/10.1016/j.jid.2022.08.037Test
https://api.elsevier.com/content/article/PII:S0022202X22018887?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0022202X22018887?httpAccept=text/plainTest

المؤلفون: Battiwalla, Minoo, Tees, Michael, Flinn, Ian W., Pantin, Jeremy, Gregory, Tara, Maris, Michael, Bhushan, Vikas, Vance, Estil, Mathews, John, Bachier, Carlos, Shaughnessy, Paul J., Ramakrishnan, Dr. Aravind, Malik, Shahbaz, Mori, Shahram, Gergis, Usama, Billups, Rocky, Blunk, Betsy, LeMaistre, Dr. C. Fred, Majhail, Navneet S.

المصدر: Transplantation and Cellular Therapy ; volume 29, issue 2, page S211 ; ISSN 2666-6367

مصطلحات موضوعية: Transplantation, Cell Biology, Hematology, Molecular Medicine, Immunology and Allergy

الإتاحة: https://doi.org/10.1016/s2666-6367Test(23)00342-1
https://api.elsevier.com/content/article/PII:S2666636723003421?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2666636723003421?httpAccept=text/plainTest

المؤلفون: Battiwalla, Minoo, Tees, Michael, Flinn, Ian W., Pantin, Jeremy, Berdeja, Jesús, Gregory, Tara, Maris, Michael, Bhushan, Vikas, Vance, Estil, Mathews, John, Bachier, Carlos, Shaughnessy, Paul J., Ramakrishnan, Dr. Aravind, Malik, Shahbaz, Mori, Shahram, Billups, Rocky, Blunk, Betsy, LeMaistre, Dr. C. Fred, Majhail, Navneet S.

المصدر: Transplantation and Cellular Therapy ; volume 29, issue 2, page S55 ; ISSN 2666-6367

مصطلحات موضوعية: Transplantation, Cell Biology, Hematology, Molecular Medicine, Immunology and Allergy

الإتاحة: https://doi.org/10.1016/s2666-6367Test(23)00133-1
https://api.elsevier.com/content/article/PII:S2666636723001331?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2666636723001331?httpAccept=text/plainTest

المؤلفون: Reves, Heather, Jackson, Meredith, Prasad, Tanushree, Afrough, Aimaz, Anderson, Larry D., Collins, Robert H., Kaur, Gurbakhash, Wolfe, Heather, Yilmaz, Elif, Khan, Adeel, Kumar, Kiran, Madanat, Yazan F., Vusirikala, Madhuri, Awan, Farrukh T, Geethakumari, Praveen Ramakrishnan

المصدر: Transplantation and Cellular Therapy ; volume 29, issue 2, page S389-S390 ; ISSN 2666-6367

مصطلحات موضوعية: Transplantation, Cell Biology, Hematology, Molecular Medicine, Immunology and Allergy

الإتاحة: https://doi.org/10.1016/s2666-6367Test(23)00588-2
https://api.elsevier.com/content/article/PII:S2666636723005882?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2666636723005882?httpAccept=text/plainTest

المؤلفون: Sannareddy, Aishwarya, Reves, Heather, Narasimhan, Madhusudhanan, Jaleta, Kefyalew, Anderson, Julia Marie, Patel, Hetalkumari, Anderson, Larry D., Afrough, Aimaz, Chung, Stephen, Collins, Robert H., Kaur, Gurbakhash, Kumar, Kiran A, Madanat, Yazan F., Vusirikala, Madhuri, Awan, Farrukh T, Muthukumar, Alagar R, Geethakumari, Praveen Ramakrishnan

المصدر: Transplantation and Cellular Therapy ; volume 29, issue 2, page S330 ; ISSN 2666-6367

مصطلحات موضوعية: Transplantation, Cell Biology, Hematology, Molecular Medicine, Immunology and Allergy

الإتاحة: https://doi.org/10.1016/s2666-6367Test(23)00505-5
https://api.elsevier.com/content/article/PII:S2666636723005055?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2666636723005055?httpAccept=text/plainTest