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1دورية أكاديمية
المؤلفون: Manola Moretti, Maria Hountondji, Rui Ge, Abdul-Hamid Emwas, Panayiotis Bilalis, Hepi H. Susapto, Abdulelah Alrashoudi, Xinzhi Liu, Giuseppina R. Briola, Charlotte A. E. Hauser
مصطلحات موضوعية: Biophysics, Medicine, Cell Biology, Genetics, Biotechnology, Developmental Biology, Inorganic Chemistry, Plant Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, Information Systems not elsewhere classified, three years since, biocompatible underwater adhesives, tunable mechanical properties, poor mechanical properties, marine environmental applications, enhance coral growth, usp hydrogel compounds, best adhesion outcome, mechanical properties, environmental applications, adhesive properties, coral regeneration, coral fragments, iizkdo hydrogel, iizdok hydrogel, hydrogel self
الوصف: Coral reef survival is threatened globally. One way to restore this delicate ecosystem is to enhance coral growth by the controlled propagation of coral fragments. To be sustainable, this technique requires the use of biocompatible underwater adhesives. Hydrogels based on rationally designed ultrashort self-assembling peptides (USP) are of great interest for various biological and environmental applications, due to their biocompatibility and tunable mechanical properties. Implementing superior adhesion properties to the USP hydrogel compounds is crucial in both water and high ionic strength solutions and is relevant in medical and marine environmental applications such as coral regeneration. Some marine animals secrete large quantities of the aminoacids dopa and lysine to enhance their adhesion to wet surfaces. Therefore, the addition of catechol moieties to the USP sequence containing lysine (IIZK) should improve the adhesive properties of USP hydrogels. However, it is challenging to place the catechol moiety (Do) within the USP sequence at an optimal position without compromising the hydrogel self-assembly process and mechanical properties. Here, we demonstrate that, among three USP hydrogels, DoIIZK is the least adhesive and that the adhesiveness of the IIZDoK hydrogel is compromised by its poor mechanical properties. The best adhesion outcome was achieved using the IIZKDo hydrogel, the only one to show equally sound adhesive and mechanical properties. A mechanistic understanding of this outcome is presented here. This property was confirmed by the successful gluing of coral fragments by means of IIZKDo hydrogel that are still thriving after more than three years since the deployment. The validated biocompatibility of this underwater hydrogel glue suggests that it could be advantageously implemented for other applications, such as surgical interventions.
الإتاحة: https://doi.org/10.1021/acs.langmuir.3c02553.s007Test
https://figshare.com/articles/journal_contribution/Selectively_Positioned_Catechol_Moiety_Supports_Ultrashort_Self-Assembling_Peptide_Hydrogel_Adhesion_for_Coral_Restoration/24713698Test -
2دورية أكاديمية
المؤلفون: Hepi H. Susapto (10032267), Dana Alhattab (10032270), Sherin Abdelrahman (10032273), Zainab Khan (4516978), Salwa Alshehri (10032276), Kowther Kahin (10032279), Rui Ge (1663630), Manola Moretti (2014258), Abdul-Hamid Emwas (1235382), Charlotte A. E. Hauser (10032282)
مصطلحات موضوعية: Biophysics, Biochemistry, Cell Biology, Molecular Biology, Pharmacology, Biotechnology, Developmental Biology, Space Science, Information Systems not elsewhere classified, tissue fabrication, structure-solidifying process, tissue scaffolds, ultrashort peptide bioinks, deposition process, peptide bioinks, extrusion procedure, encapsulated cells, chondrogenic differentiation, bioprinting procedures, tissue engineering, Large-Scale Constructs Assuring Lon., shape fidelity, cell-embedding printing process, bioink concentrations, structure complexity, cell survival, cell viability, Printed Tissue Constructs, cells point, Ultrashort Peptide Bioinks Support .
الوصف: We report about rationally designed ultrashort peptide bioinks, overcoming severe limitations in current bioprinting procedures. Bioprinting is increasingly relevant in tissue engineering, regenerative and personalized medicine due to its ability to fabricate complex tissue scaffolds through an automated deposition process. Printing stable large-scale constructs with high shape fidelity and enabling long-term cell survival are major challenges that most existing bioinks are unable to solve. Additionally, they require chemical or UV-cross-linking for the structure-solidifying process which compromises the encapsulated cells, resulting in restricted structure complexity and low cell viability. Using ultrashort peptide bioinks as ideal bodylike but synthetic material, we demonstrate an instant solidifying cell-embedding printing process via a sophisticated extrusion procedure under true physiological conditions and at cost-effective low bioink concentrations. Our printed large-scale cell constructs and the chondrogenic differentiation of printed mesenchymal stem cells point to the strong potential of the peptide bioinks for automated complex tissue fabrication.
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3
المؤلفون: Manola Moretti, Maria Hountondji, Rui Ge, Abdul-Hamid Emwas, Panayiotis Bilalis, Hepi H. Susapto, Abdulelah Alrashoudi, Xinzhi Liu, Giuseppina R. Briola, Charlotte A. E. Hauser
مصطلحات موضوعية: Biophysics, Medicine, Cell Biology, Genetics, Biotechnology, Developmental Biology, Inorganic Chemistry, Plant Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, Information Systems not elsewhere classified, three years since, biocompatible underwater adhesives, tunable mechanical properties, poor mechanical properties, marine environmental applications, enhance coral growth, usp hydrogel compounds, best adhesion outcome, mechanical properties, environmental applications, adhesive properties, coral regeneration, coral fragments, iizkdo hydrogel, iizdok hydrogel, hydrogel self
الوصف: Coral reef survival is threatened globally. One way to restore this delicate ecosystem is to enhance coral growth by the controlled propagation of coral fragments. To be sustainable, this technique requires the use of biocompatible underwater adhesives. Hydrogels based on rationally designed ultrashort self-assembling peptides (USP) are of great interest for various biological and environmental applications, due to their biocompatibility and tunable mechanical properties. Implementing superior adhesion properties to the USP hydrogel compounds is crucial in both water and high ionic strength solutions and is relevant in medical and marine environmental applications such as coral regeneration. Some marine animals secrete large quantities of the aminoacids dopa and lysine to enhance their adhesion to wet surfaces. Therefore, the addition of catechol moieties to the USP sequence containing lysine (IIZK) should improve the adhesive properties of USP hydrogels. However, it is challenging to place the catechol moiety (Do) within the USP sequence at an optimal position without compromising the hydrogel self-assembly process and mechanical properties. Here, we demonstrate that, among three USP hydrogels, DoIIZK is the least adhesive and that the adhesiveness of the IIZDoK hydrogel is compromised by its poor mechanical properties. The best adhesion outcome was achieved using the IIZKDo hydrogel, the only one to show equally sound adhesive and mechanical properties. A mechanistic understanding of this outcome is presented here. This property was confirmed by the successful gluing of coral fragments by means of IIZKDo hydrogel that are still thriving after more than three years since the deployment. The validated biocompatibility of this underwater hydrogel glue suggests that it could be advantageously implemented for other applications, such as surgical interventions.
الإتاحة: https://doi.org/10.1021/acs.langmuir.3c02553.s003Test
https://figshare.com/articles/media/Selectively_Positioned_Catechol_Moiety_Supports_Ultrashort_Self-Assembling_Peptide_Hydrogel_Adhesion_for_Coral_Restoration/24713683Test -
4
المؤلفون: Manola Moretti, Maria Hountondji, Rui Ge, Abdul-Hamid Emwas, Panayiotis Bilalis, Hepi H. Susapto, Abdulelah Alrashoudi, Xinzhi Liu, Giuseppina R. Briola, Charlotte A. E. Hauser
مصطلحات موضوعية: Biophysics, Medicine, Cell Biology, Genetics, Biotechnology, Developmental Biology, Inorganic Chemistry, Plant Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, Information Systems not elsewhere classified, three years since, biocompatible underwater adhesives, tunable mechanical properties, poor mechanical properties, marine environmental applications, enhance coral growth, usp hydrogel compounds, best adhesion outcome, mechanical properties, environmental applications, adhesive properties, coral regeneration, coral fragments, iizkdo hydrogel, iizdok hydrogel, hydrogel self
الوصف: Coral reef survival is threatened globally. One way to restore this delicate ecosystem is to enhance coral growth by the controlled propagation of coral fragments. To be sustainable, this technique requires the use of biocompatible underwater adhesives. Hydrogels based on rationally designed ultrashort self-assembling peptides (USP) are of great interest for various biological and environmental applications, due to their biocompatibility and tunable mechanical properties. Implementing superior adhesion properties to the USP hydrogel compounds is crucial in both water and high ionic strength solutions and is relevant in medical and marine environmental applications such as coral regeneration. Some marine animals secrete large quantities of the aminoacids dopa and lysine to enhance their adhesion to wet surfaces. Therefore, the addition of catechol moieties to the USP sequence containing lysine (IIZK) should improve the adhesive properties of USP hydrogels. However, it is challenging to place the catechol moiety (Do) within the USP sequence at an optimal position without compromising the hydrogel self-assembly process and mechanical properties. Here, we demonstrate that, among three USP hydrogels, DoIIZK is the least adhesive and that the adhesiveness of the IIZDoK hydrogel is compromised by its poor mechanical properties. The best adhesion outcome was achieved using the IIZKDo hydrogel, the only one to show equally sound adhesive and mechanical properties. A mechanistic understanding of this outcome is presented here. This property was confirmed by the successful gluing of coral fragments by means of IIZKDo hydrogel that are still thriving after more than three years since the deployment. The validated biocompatibility of this underwater hydrogel glue suggests that it could be advantageously implemented for other applications, such as surgical interventions.
الإتاحة: https://doi.org/10.1021/acs.langmuir.3c02553.s002Test
https://figshare.com/articles/media/Selectively_Positioned_Catechol_Moiety_Supports_Ultrashort_Self-Assembling_Peptide_Hydrogel_Adhesion_for_Coral_Restoration/24713680Test -
5
المؤلفون: Manola Moretti, Maria Hountondji, Rui Ge, Abdul-Hamid Emwas, Panayiotis Bilalis, Hepi H. Susapto, Abdulelah Alrashoudi, Xinzhi Liu, Giuseppina R. Briola, Charlotte A. E. Hauser
مصطلحات موضوعية: Biophysics, Medicine, Cell Biology, Genetics, Biotechnology, Developmental Biology, Inorganic Chemistry, Plant Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, Information Systems not elsewhere classified, three years since, biocompatible underwater adhesives, tunable mechanical properties, poor mechanical properties, marine environmental applications, enhance coral growth, usp hydrogel compounds, best adhesion outcome, mechanical properties, environmental applications, adhesive properties, coral regeneration, coral fragments, iizkdo hydrogel, iizdok hydrogel, hydrogel self
الوصف: Coral reef survival is threatened globally. One way to restore this delicate ecosystem is to enhance coral growth by the controlled propagation of coral fragments. To be sustainable, this technique requires the use of biocompatible underwater adhesives. Hydrogels based on rationally designed ultrashort self-assembling peptides (USP) are of great interest for various biological and environmental applications, due to their biocompatibility and tunable mechanical properties. Implementing superior adhesion properties to the USP hydrogel compounds is crucial in both water and high ionic strength solutions and is relevant in medical and marine environmental applications such as coral regeneration. Some marine animals secrete large quantities of the aminoacids dopa and lysine to enhance their adhesion to wet surfaces. Therefore, the addition of catechol moieties to the USP sequence containing lysine (IIZK) should improve the adhesive properties of USP hydrogels. However, it is challenging to place the catechol moiety (Do) within the USP sequence at an optimal position without compromising the hydrogel self-assembly process and mechanical properties. Here, we demonstrate that, among three USP hydrogels, DoIIZK is the least adhesive and that the adhesiveness of the IIZDoK hydrogel is compromised by its poor mechanical properties. The best adhesion outcome was achieved using the IIZKDo hydrogel, the only one to show equally sound adhesive and mechanical properties. A mechanistic understanding of this outcome is presented here. This property was confirmed by the successful gluing of coral fragments by means of IIZKDo hydrogel that are still thriving after more than three years since the deployment. The validated biocompatibility of this underwater hydrogel glue suggests that it could be advantageously implemented for other applications, such as surgical interventions.
الإتاحة: https://doi.org/10.1021/acs.langmuir.3c02553.s001Test
https://figshare.com/articles/media/Selectively_Positioned_Catechol_Moiety_Supports_Ultrashort_Self-Assembling_Peptide_Hydrogel_Adhesion_for_Coral_Restoration/24713677Test -
6
المؤلفون: Manola Moretti, Maria Hountondji, Rui Ge, Abdul-Hamid Emwas, Panayiotis Bilalis, Hepi H. Susapto, Abdulelah Alrashoudi, Xinzhi Liu, Giuseppina R. Briola, Charlotte A. E. Hauser
مصطلحات موضوعية: Biophysics, Medicine, Cell Biology, Genetics, Biotechnology, Developmental Biology, Inorganic Chemistry, Plant Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, Information Systems not elsewhere classified, three years since, biocompatible underwater adhesives, tunable mechanical properties, poor mechanical properties, marine environmental applications, enhance coral growth, usp hydrogel compounds, best adhesion outcome, mechanical properties, environmental applications, adhesive properties, coral regeneration, coral fragments, iizkdo hydrogel, iizdok hydrogel, hydrogel self
الوصف: Coral reef survival is threatened globally. One way to restore this delicate ecosystem is to enhance coral growth by the controlled propagation of coral fragments. To be sustainable, this technique requires the use of biocompatible underwater adhesives. Hydrogels based on rationally designed ultrashort self-assembling peptides (USP) are of great interest for various biological and environmental applications, due to their biocompatibility and tunable mechanical properties. Implementing superior adhesion properties to the USP hydrogel compounds is crucial in both water and high ionic strength solutions and is relevant in medical and marine environmental applications such as coral regeneration. Some marine animals secrete large quantities of the aminoacids dopa and lysine to enhance their adhesion to wet surfaces. Therefore, the addition of catechol moieties to the USP sequence containing lysine (IIZK) should improve the adhesive properties of USP hydrogels. However, it is challenging to place the catechol moiety (Do) within the USP sequence at an optimal position without compromising the hydrogel self-assembly process and mechanical properties. Here, we demonstrate that, among three USP hydrogels, DoIIZK is the least adhesive and that the adhesiveness of the IIZDoK hydrogel is compromised by its poor mechanical properties. The best adhesion outcome was achieved using the IIZKDo hydrogel, the only one to show equally sound adhesive and mechanical properties. A mechanistic understanding of this outcome is presented here. This property was confirmed by the successful gluing of coral fragments by means of IIZKDo hydrogel that are still thriving after more than three years since the deployment. The validated biocompatibility of this underwater hydrogel glue suggests that it could be advantageously implemented for other applications, such as surgical interventions.
الإتاحة: https://doi.org/10.1021/acs.langmuir.3c02553.s005Test
https://figshare.com/articles/media/Selectively_Positioned_Catechol_Moiety_Supports_Ultrashort_Self-Assembling_Peptide_Hydrogel_Adhesion_for_Coral_Restoration/24713692Test -
7
المؤلفون: Manola Moretti, Maria Hountondji, Rui Ge, Abdul-Hamid Emwas, Panayiotis Bilalis, Hepi H. Susapto, Abdulelah Alrashoudi, Xinzhi Liu, Giuseppina R. Briola, Charlotte A. E. Hauser
مصطلحات موضوعية: Biophysics, Medicine, Cell Biology, Genetics, Biotechnology, Developmental Biology, Inorganic Chemistry, Plant Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, Information Systems not elsewhere classified, three years since, biocompatible underwater adhesives, tunable mechanical properties, poor mechanical properties, marine environmental applications, enhance coral growth, usp hydrogel compounds, best adhesion outcome, mechanical properties, environmental applications, adhesive properties, coral regeneration, coral fragments, iizkdo hydrogel, iizdok hydrogel, hydrogel self
الوصف: Coral reef survival is threatened globally. One way to restore this delicate ecosystem is to enhance coral growth by the controlled propagation of coral fragments. To be sustainable, this technique requires the use of biocompatible underwater adhesives. Hydrogels based on rationally designed ultrashort self-assembling peptides (USP) are of great interest for various biological and environmental applications, due to their biocompatibility and tunable mechanical properties. Implementing superior adhesion properties to the USP hydrogel compounds is crucial in both water and high ionic strength solutions and is relevant in medical and marine environmental applications such as coral regeneration. Some marine animals secrete large quantities of the aminoacids dopa and lysine to enhance their adhesion to wet surfaces. Therefore, the addition of catechol moieties to the USP sequence containing lysine (IIZK) should improve the adhesive properties of USP hydrogels. However, it is challenging to place the catechol moiety (Do) within the USP sequence at an optimal position without compromising the hydrogel self-assembly process and mechanical properties. Here, we demonstrate that, among three USP hydrogels, DoIIZK is the least adhesive and that the adhesiveness of the IIZDoK hydrogel is compromised by its poor mechanical properties. The best adhesion outcome was achieved using the IIZKDo hydrogel, the only one to show equally sound adhesive and mechanical properties. A mechanistic understanding of this outcome is presented here. This property was confirmed by the successful gluing of coral fragments by means of IIZKDo hydrogel that are still thriving after more than three years since the deployment. The validated biocompatibility of this underwater hydrogel glue suggests that it could be advantageously implemented for other applications, such as surgical interventions.
الإتاحة: https://doi.org/10.1021/acs.langmuir.3c02553.s004Test
https://figshare.com/articles/media/Selectively_Positioned_Catechol_Moiety_Supports_Ultrashort_Self-Assembling_Peptide_Hydrogel_Adhesion_for_Coral_Restoration/24713689Test -
8
المؤلفون: Manola Moretti, Maria Hountondji, Rui Ge, Abdul-Hamid Emwas, Panayiotis Bilalis, Hepi H. Susapto, Abdulelah Alrashoudi, Xinzhi Liu, Giuseppina R. Briola, Charlotte A. E. Hauser
مصطلحات موضوعية: Biophysics, Medicine, Cell Biology, Genetics, Biotechnology, Developmental Biology, Inorganic Chemistry, Plant Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, Information Systems not elsewhere classified, three years since, biocompatible underwater adhesives, tunable mechanical properties, poor mechanical properties, marine environmental applications, enhance coral growth, usp hydrogel compounds, best adhesion outcome, mechanical properties, environmental applications, adhesive properties, coral regeneration, coral fragments, iizkdo hydrogel, iizdok hydrogel, hydrogel self
الوصف: Coral reef survival is threatened globally. One way to restore this delicate ecosystem is to enhance coral growth by the controlled propagation of coral fragments. To be sustainable, this technique requires the use of biocompatible underwater adhesives. Hydrogels based on rationally designed ultrashort self-assembling peptides (USP) are of great interest for various biological and environmental applications, due to their biocompatibility and tunable mechanical properties. Implementing superior adhesion properties to the USP hydrogel compounds is crucial in both water and high ionic strength solutions and is relevant in medical and marine environmental applications such as coral regeneration. Some marine animals secrete large quantities of the aminoacids dopa and lysine to enhance their adhesion to wet surfaces. Therefore, the addition of catechol moieties to the USP sequence containing lysine (IIZK) should improve the adhesive properties of USP hydrogels. However, it is challenging to place the catechol moiety (Do) within the USP sequence at an optimal position without compromising the hydrogel self-assembly process and mechanical properties. Here, we demonstrate that, among three USP hydrogels, DoIIZK is the least adhesive and that the adhesiveness of the IIZDoK hydrogel is compromised by its poor mechanical properties. The best adhesion outcome was achieved using the IIZKDo hydrogel, the only one to show equally sound adhesive and mechanical properties. A mechanistic understanding of this outcome is presented here. This property was confirmed by the successful gluing of coral fragments by means of IIZKDo hydrogel that are still thriving after more than three years since the deployment. The validated biocompatibility of this underwater hydrogel glue suggests that it could be advantageously implemented for other applications, such as surgical interventions.
الإتاحة: https://doi.org/10.1021/acs.langmuir.3c02553.s006Test
https://figshare.com/articles/media/Selectively_Positioned_Catechol_Moiety_Supports_Ultrashort_Self-Assembling_Peptide_Hydrogel_Adhesion_for_Coral_Restoration/24713695Test -
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مصطلحات موضوعية: Biophysics, Biochemistry, Medicine, Cell Biology, Biotechnology, Ecology, Developmental Biology, Computational Biology, Space Science, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, 3 D cell culture applications, HUVEC, self-assembling tetrapeptide scaffolds, Mesenchymal Stem Cells, amphiphilic self-assembling peptides, bone disorders demands, ultrashort peptide hydrogels, peptide hydrogels, Self-Assembling Tetrapeptides Suppo., bone tissue engineering
الوصف: The apparent rise of bone disorders demands advanced treatment protocols involving tissue engineering. Here, we describe self-assembling tetrapeptide scaffolds for the growth and osteogenic differentiation of human mesenchymal stem cells (hMSCs). The rationally designed peptides are synthetic amphiphilic self-assembling peptides composed of four amino acids that are nontoxic. These tetrapeptides can quickly solidify to nanofibrous hydrogels that resemble the extracellular matrix and provide a three-dimensional (3D) environment for cells with suitable mechanical properties. Furthermore, we can easily tune the stiffness of these peptide hydrogels by just increasing the peptide concentration, thus providing a wide range of peptide hydrogels with different stiffnesses for 3D cell culture applications. Since successful bone regeneration requires both osteogenesis and vascularization, our scaffold was found to be able to promote angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro . The results presented suggest that ultrashort peptide hydrogels are promising candidates for applications in bone tissue engineering.
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10
مصطلحات موضوعية: Biophysics, Biochemistry, Medicine, Cell Biology, Biotechnology, Ecology, Developmental Biology, Computational Biology, Space Science, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, 3 D cell culture applications, HUVEC, self-assembling tetrapeptide scaffolds, Mesenchymal Stem Cells, amphiphilic self-assembling peptides, bone disorders demands, ultrashort peptide hydrogels, peptide hydrogels, Self-Assembling Tetrapeptides Suppo., bone tissue engineering
الوصف: The apparent rise of bone disorders demands advanced treatment protocols involving tissue engineering. Here, we describe self-assembling tetrapeptide scaffolds for the growth and osteogenic differentiation of human mesenchymal stem cells (hMSCs). The rationally designed peptides are synthetic amphiphilic self-assembling peptides composed of four amino acids that are nontoxic. These tetrapeptides can quickly solidify to nanofibrous hydrogels that resemble the extracellular matrix and provide a three-dimensional (3D) environment for cells with suitable mechanical properties. Furthermore, we can easily tune the stiffness of these peptide hydrogels by just increasing the peptide concentration, thus providing a wide range of peptide hydrogels with different stiffnesses for 3D cell culture applications. Since successful bone regeneration requires both osteogenesis and vascularization, our scaffold was found to be able to promote angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro . The results presented suggest that ultrashort peptide hydrogels are promising candidates for applications in bone tissue engineering.
