Crambescidin-816 Acts as a Fungicidal with More Potency than Crambescidin-800 and -830, Inducing Cell Cycle Arrest, Increased Cell Size and Apoptosis in Saccharomyces cerevisiae
| العنوان: | Crambescidin-816 Acts as a Fungicidal with More Potency than Crambescidin-800 and -830, Inducing Cell Cycle Arrest, Increased Cell Size and Apoptosis in Saccharomyces cerevisiae |
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| المؤلفون: | Olivier P. Thomas, Mercedes R. Vieytes, Juan A. Rubiolo, F. V. Vega, H. López-Alonso, Eva Ternon, Luis M. Botana |
| المساهمون: | Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Universidade de Santiago de Compostela. Departamento de Fisioloxía |
| المصدر: | Marine Drugs; Volume 11; Issue 11; Pages: 4419-4434 Marine Drugs Minerva: Repositorio Institucional de la Universidad de Santiago de Compostela Universidad de Santiago de Compostela (USC) Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname Marine Drugs, Vol 11, Iss 11, Pp 4419-4434 (2013) |
| بيانات النشر: | MDPI AG, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | G2 Phase, Programmed cell death, Cell cycle checkpoint, Cell division, Saccharomyces cerevisiae, Pharmaceutical Science, Apoptosis, crambescidine-816, Antifungal, Article, Cell cycle arrest, 03 medical and health sciences, chemistry.chemical_compound, Alkaloids, 0302 clinical medicine, Drug Discovery, Spiro Compounds, lcsh:QH301-705.5, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), Guanidine, Cell Size, 030304 developmental biology, Membrane Potential, Mitochondrial, 0303 health sciences, Crambescidine-816, Cell Death, biology, apoptosis, Cell Cycle Checkpoints, Cell cycle, biology.organism_classification, Yeast, Fungicides, Industrial, 3. Good health, Cell biology, lcsh:Biology (General), Biochemistry, chemistry, cell cycle arrest, 030220 oncology & carcinogenesis, antifungal, Cell Division, DNA |
| الوصف: | In this paper, we show the effect of crambescidin-816, -800, and -830 on Saccharomyces cerevisiae viability. We determined that, of the three molecules tested, crambescidin-816 was the most potent. Based on this result, we continued by determining the effect of crambescidin-816 on the cell cycle of this yeast. The compound induced cell cycle arrest in G2/M followed by an increase in cell DNA content and size. When the type of cell death was analyzed, we observed that crambescidin-816 induced apoptosis. The antifungal effect indicates that crambescidins, and mostly crambescidin-816, could serve as a lead compound to fight fungal infections The research leading to these results has received funding from the following FEDER cofunded-grants: From Ministerio de Ciencia y Tecnología, Spain: AGL2009-13581-CO2-01, AGL2012-40485-CO2-01. From Xunta de Galicia, Spain: 10PXIB261254 PR. From the European Union’s Seventh Framework Programme managed by REA—Research Executive Agency (FP7/2007-2013) under grant agreement Nos. 211326—CP (CONffIDENCE), 265896 BAMMBO, 265409 µAQUA, and 262649 BEADS, 315285 Ciguatools and 312184 PharmaSea. From the Atlantic Area Programme (Interreg IVB Trans-national): 2009-1/117 Pharmatlantic SI |
| وصف الملف: | application/pdf |
| تدمد: | 1660-3397 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9cf4f7648931adf32694ebcf0c3d93d3Test https://doi.org/10.3390/md11114419Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9cf4f7648931adf32694ebcf0c3d93d3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16603397 |
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