التفاصيل البيبلوغرافية
| العنوان: |
Myocardial Structural and Biological Anomalies Induced by High Fat Diet in Psammomys obesus Gerbils. |
| المؤلفون: |
Sahraoui, Abdelhamid, Dewachter, Céline, de Medina, Geoffrey, Naeije, Robert, Aouichat Bouguerra, Souhila, Dewachter, Laurence |
| المصدر: |
PLoS ONE; 2/3/2016, Vol. 11 Issue 2, p1-16, 16p |
| مصطلحات موضوعية: |
MYOCARDIAL infarction diagnosis, HIGH-fat diet, PSAMMOMYS obesus, RODENT diseases, OBESITY in animals, HEART function tests |
| مستخلص: |
Background: Psammomys obesus gerbils are particularly prone to develop diabetes and obesity after brief period of abundant food intake. A hypercaloric high fat diet has been shown to affect cardiac function. Here, we sought to determine whether a short period of high fat feeding might alter myocardial structure and expression of calcium handling proteins in this particular strain of gerbils. Methods: Twenty Psammomys obesus gerbils were randomly assigned to receive a normal plant diet (controls) or a high fat diet. At baseline and 16-week later, body weight, plasma biochemical parameters (including lipid and carbohydrate levels) were evaluated. Myocardial samples were collected for pathobiological evaluation. Results: Sixteen-week high fat dieting resulted in body weight gain and hyperlipidemia, while levels of carbohydrates remained unchanged. At myocardial level, high fat diet induced structural disorganization, including cardiomyocyte hypertrophy, lipid accumulation, interstitial and perivascular fibrosis and increased number of infiltrating neutrophils. Myocardial expressions of pro-apoptotic Bax-to-Bcl-2 ratio, pro-inflammatory cytokines [interleukin (IL)-1β and tumor necrosis factor (TNF)-α], intercellular (ICAM1) and vascular adhesion molecules (VCAM1) increased, while gene encoding cardiac muscle protein, the alpha myosin heavy polypeptide (MYH6), was downregulated. Myocardial expressions of sarco(endo)plasmic calcium-ATPase (SERCA2) and voltage-dependent calcium channel (Cacna1c) decreased, while protein kinase A (PKA) and calcium-calmodulin-dependent protein kinase (CaMK2D) expressions increased. Myocardial expressions of ryanodine receptor, phospholamban and sodium/calcium exchanger (Slc8a1) did not change. Conclusions: We conclude that a relative short period of high fat diet in Psammomys obesus results in severe alterations of cardiac structure, activation of inflammatory and apoptotic processes, and altered expression of calcium-cycling determinants. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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