Effects of the deficiency of the rhodanese-like protein RhdA in Azotobacter vinelandii
| العنوان: | Effects of the deficiency of the rhodanese-like protein RhdA in Azotobacter vinelandii |
|---|---|
| المؤلفون: | Franco Faoro, Marcello Iriti, Gianluca Picariello, Aristodemo Carpen, Pasquale Ferranti, Silvia Pagani, Angelo Cereda |
| المساهمون: | Cereda, A, Carpen, A, Picariello, G, Iriti, M, Faoro, F, Ferranti, Pasquale, Pagani, S. |
| المصدر: | FEBS letters 581 (2007): 1625–1630. info:cnr-pdr/source/autori:Cereda A, Carpen A, Picariello G, Iriti M, Faoro F, Ferranti P, Pagani S./titolo:Effects of the deficiency of the rhodanese-like protein RhdA in Azotobacter vinelandii/doi:/rivista:FEBS letters (Print)/anno:2007/pagina_da:1625/pagina_a:1630/intervallo_pagine:1625–1630/volume:581 |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Proteomics, Operon, Mutant, Biophysics, Rhodanese, Biochemistry, Aconitase, Polyhydroxybutyrate, Bacterial Proteins, Structural Biology, Settore BIO/10 - Biochimica, Genetics, Electrophoresis, Gel, Two-Dimensional, Molecular Biology, chemistry.chemical_classification, Azotobacter vinelandii, biology, rhdA mutant, Settore AGR/12 - Patologia Vegetale, Cell Biology, biology.organism_classification, Oxidants, Thiosulfate Sulfurtransferase, Citric acid cycle, Oxidative Stress, Enzyme, Phenotype, chemistry, Genes, Bacterial, Mutation, Methylphenazonium Methosulfate, bacteria, Protein profiles |
| الوصف: | In Azotobacter vinelandii the rhdA gene codes for a protein (RhdA) of the rhodanese-homology superfamily. By combining proteomics, enzymic profiles and ultrastructural observations, the phenotype of an A. vinelandii rhdA mutant was analyzed. We found that the A. vinelandii rhdA mutant, and not the wild-type strain, accumulated polyhydroxybutyrate. RhdA deficiency enhanced the expression of enzymes of the polyhydroxybutyrate biosynthetic operon, and affected the activity of specific tricarboxylic acid cycle enzymes. The effect was dramatic on aconitase, in spite of comparable expression of aconitase polypeptides in both strains. By using a model system, we found that RhdA triggered protection from oxidants. |
| وصف الملف: | STAMPA |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbb42c52aefa7d9a8130596bc263b8deTest http://hdl.handle.net/2434/41611Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bbb42c52aefa7d9a8130596bc263b8de |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |