Cytotoxic Mechanism ofCytolethal Distending Toxinin NontyphoidalSalmonellaSerovar (SalmonellaJaviana) During Macrophage Infection
| العنوان: | Cytotoxic Mechanism ofCytolethal Distending Toxinin NontyphoidalSalmonellaSerovar (SalmonellaJaviana) During Macrophage Infection |
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| المؤلفون: | Kuppan Gokulan, Katherine Williams, Sangeeta Khare, Ashraf A. Khan, Diamond Shelman, Tatsuya Akiyama |
| المصدر: | DNA and Cell Biology. 34:113-124 |
| بيانات النشر: | Mary Ann Liebert Inc, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Salmonella, Cytolethal distending toxin, Cell Survival, Bacterial Toxins, Gene Expression, Apoptosis, Biology, medicine.disease_cause, Virulence factor, Cell Line, Microbiology, Mice, Necrosis, Phosphatidylinositol 3-Kinases, Autophagy, Genetics, medicine, Animals, Cytotoxic T cell, Cell Shape, Molecular Biology, bcl-2-Associated X Protein, Reverse Transcriptase Polymerase Chain Reaction, Toxin, Macrophages, Estrogen Receptor alpha, Wild type, Cell Biology, General Medicine, Deubiquitinating Enzyme CYLD, Cysteine Endopeptidases, Microscopy, Fluorescence, Host-Pathogen Interactions, Mutation, Microtubule-Associated Proteins |
| الوصف: | Cytolethal distending toxin B (cdtB) is a conserved virulence factor in Salmonella enterica serovar Typhi. Here we report the presence and functionality of cdtB in some nontyphoidal Salmonella (NTS) serovars, including Salmonella Javiana (cdtB+wt S. Javiana), isolated from imported food. To understand the role of cdtB in NTS serovars, a deletion mutant (cdtB(-)ΔS. Javiana) was constructed. Macrophages were infected with cdtB+wt S. Javiana (wild type), cdtB(-)Δ S. Javiana (mutant), and cdtB-negative NTS serovar (S. Typhimurium). Cytotoxic activity and transcription level of genes involved in cell death (apoptosis, autophagy, and necrosis) were assessed in infected macrophages. The cdtB+wt S. Javiana caused cellular distension as well as high degree of vacuolization and presence of the autophagosome marker LC3 in infected macrophages as compared with cdtB(-)ΔS. Javiana. The mRNA expression of genes involved in the induction of autophagy in response to toxin (Esr1 and Pik3C3) and coregulators of autophagy and apoptosis (Bax and Cyld) were significantly upregulated in cdtB(+)wt S. Javiana-infected macrophages. As autophagy destroys internalized pathogens in addition to the infected cell, it may reduce the spread of infection. |
| تدمد: | 1557-7430 1044-5498 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0df1476cc5e4586f6ad34ab1a6d36536Test https://doi.org/10.1089/dna.2014.2602Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....0df1476cc5e4586f6ad34ab1a6d36536 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15577430 10445498 |
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