Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action
| العنوان: | Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action |
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| المؤلفون: | Matjaž Stenovec, Eva Lasič, Marjeta Lisjak, Nina Vardjan, Aleksandra Šakanović, Jernej Jorgačevski, Alexei Verkhratsky, Gregor Anderluh, Anemari Horvat, Mićo Božić, Robert Zorec |
| المصدر: | Lasic, E, Lisjak, M, Horvat, A, Bozic, M, Sakanovic, A, Anderluh, G, Verkhratsky, A, Vardjan, N, Jorgacevski, J, Stenovec, M & Zorec, R 2019, ' Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action ', Scientific Reports, vol. 9, 10957 . https://doi.org/10.1038/s41598-019-47459-zTest Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) Scientific Reports Lasič, E, Lisjak, M, Horvat, A, Božić, M, Šakanović, A, Anderluh, G, Verkhratsky, A, Vardjan, N, Jorgačevski, J, Stenovec, M & Zorec, R 2019, ' Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action ', Scientific Reports, vol. 9, no. 1, 10957 . https://doi.org/10.1038/s41598-019-47459-zTest Scientific reports, vol. 9, 10957, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, PC12 Cells, chemistry.chemical_compound, 0302 clinical medicine, Cyclic AMP, Membrane lipids, Membrane potential, Multidisciplinary, Membrane fission, Antidepressive Agents, Cell biology, medicine.anatomical_structure, Cholesterol, ketamin, Antidepressant, Medicine, Female, Ketamine, Astrocyte, medicine.drug, ketamine, Science, Membrane fusion, Exocytosis, Article, 03 medical and health sciences, holesterol, medicine, Animals, Secretion, Rats, Wistar, General, astrociti, Depressive Disorder, Major, Cell Membrane, astrocytes, cholesterol, Rats, 030104 developmental biology, Förster resonance energy transfer, chemistry, Astrocytes, udc:616-092, 030217 neurology & neurosurgery |
| الوصف: | Ketamine is an antidepressant with rapid therapeutic onset and long-lasting effect, although the underlying mechanism(s) remain unknown. Using FRET-based nanosensors we found that ketamine increases [cAMP]i in astrocytes. Membrane capacitance recordings, however, reveal fundamentally distinct mechanisms of effects of ketamine and [cAMP]i on vesicular secretion: a rise in [cAMP]i facilitated, whereas ketamine inhibited exocytosis. By directly monitoring cholesterol-rich membrane domains with a fluorescently tagged cholesterol-specific membrane binding domain (D4) of toxin perfringolysin O, we demonstrated that ketamine induced cholesterol redistribution in the plasmalemma in astrocytes, but neither in fibroblasts nor in PC 12 cells. This novel mechanism posits that ketamine affects density and distribution of cholesterol in the astrocytic plasmalemma, consequently modulating a host of processes that may contribute to ketamine’s rapid antidepressant action. |
| وصف الملف: | application/pdf; text/url |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10b382663d4b4d8153226679010063f4Test https://curis.ku.dk/ws/files/225553722/Astrocyte_Specific_Remodeling_of_Plasmalemmal_Cholesterol_Composition_by_Ketamine_Indicates_a_New_Mechanism_of_Antidepressant_Action.pdfTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....10b382663d4b4d8153226679010063f4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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