Immunocompetence of macrophages in rats exposed toCandida albicansinfection and stress
| العنوان: | Immunocompetence of macrophages in rats exposed toCandida albicansinfection and stress |
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| المؤلفون: | Silvia G. Correa, Beatriz Salido Renterı́a, Ana María Masini-Repiso, Maria Eugenia Costamagna, Claudia Elena Sotomayor, Maria Cecilia Rodriguez-Galan, Ana M. Cabanillas |
| المصدر: | American Journal of Physiology-Cell Physiology. 284:C111-C118 |
| بيانات النشر: | American Physiological Society, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Cytotoxicity, Immunologic, Cellular immunity, Physiology, medicine.medical_treatment, Biology, Immune system, Phagocytosis, Stress, Physiological, Candida albicans, medicine, Animals, Macrophage, RNA, Messenger, Rats, Wistar, Tumor Necrosis Factor-alpha, Macrophages, Candidiasis, Cell Biology, biology.organism_classification, Corpus albicans, Rats, Cytokine, Immunology, Female, Tumor necrosis factor alpha, Immunocompetence, Corticosterone, Interleukin-1 |
| الوصف: | The integration of innate and adaptive immune responses is required for efficient control of Candida albicans. The present work aimed to assess, at the local site of the infection, the immunocompetence of macrophages in rats infected intraperitoneally with C. albicans and exposed simultaneously to stress during 3 days (CaS group). We studied the 1) ability to remove and kill C. albicans, 2) tumor necrosis factor-α (TNF-α) release, 3) balance of the inducible enzymes NO synthase (iNOS) and arginase, and 4) expression of interleukin (IL)-1β and IL-1 receptor antagonist (ra) mRNA. Compared with only infected animals (Ca group), the number of colony-forming units was significantly higher in CaS rats ( P < 0.01), and the macrophage candidicidal activity was ∼2.5-fold lower ( P < 0.01). Release of TNF-α was diminished in both unstimulated and heat-killed C. albicans restimulated macrophages of the CaS group (Ca vs. CaS, P < 0.03 and P < 0.05, respectively). In Ca- and CaS-group rats, the rates for both the arginase activity and the NO synthesis were significantly enhanced. However, the stress exposure downregulated the activity of both enzymes (CaS vs. Ca, P < 0.05). After in vitro restimulation, the IL-1ra/IL-1β ratio was significantly diminished in CaS-group rats ( P < 0.05). Our results indicate that a correlation exists between early impairment of macrophage function and stress exposure. |
| تدمد: | 1522-1563 0363-6143 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd3d47d2ad1dd097f21ef99f84cc475bTest https://doi.org/10.1152/ajpcell.00160.2002Test |
| رقم الانضمام: | edsair.doi.dedup.....fd3d47d2ad1dd097f21ef99f84cc475b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221563 03636143 |
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