التفاصيل البيبلوغرافية
| العنوان: |
A Positive Regulatory Feedback Loop between EKLF/KLF1 and TAL1/SCL Sustaining the Erythropoiesis. |
| المؤلفون: |
Hung, Chun-Hao1,2 (AUTHOR) libur777@gate.sinica.edu.tw, Lee, Tung-Liang1 (AUTHOR) andywork0711@gmail.com, Huang, Anna Yu-Szu1,3 (AUTHOR) annabellehuang7645@gmail.com, Yang, Kang-Chung4,5 (AUTHOR) kcyang@stat.sinica.edu.tw, Shyu, Yu-Chiau1,6,7 (AUTHOR) yuchiaushyu@gmail.com, Wen, Shau-Ching1 (AUTHOR) sophiweng62@gmail.com, Lu, Mu-Jie1,2 (AUTHOR) ruru79320@gmail.com, Yuan, Shinsheng5 (AUTHOR) syuan@stat.sinica.edu.tw, Shen, Che-Kun James1,3,8 (AUTHOR) syuan@stat.sinica.edu.tw |
| المصدر: |
International Journal of Molecular Sciences. Aug2021, Vol. 22 Issue 15, p8024-8024. 1p. |
| مصطلحات موضوعية: |
*GENE expression profiling, *FETAL liver cells, *ERYTHROPOIESIS, *TRANSCRIPTION factors, *CELL analysis |
| مستخلص: |
The erythroid Krüppel-like factor EKLF/KLF1 is a hematopoietic transcription factor binding to the CACCC DNA motif and participating in the regulation of erythroid differentiation. With combined use of microarray-based gene expression profiling and the promoter-based ChIP-chip assay of E14.5 fetal liver cells from wild type (WT) and EKLF-knockout (Eklf−/−) mouse embryos, we identified the pathways and direct target genes activated or repressed by EKLF. This genome-wide study together with the molecular/cellular analysis of the mouse erythroleukemic cells (MEL) indicate that among the downstream direct target genes of EKLF is Tal1/Scl. Tal1/Scl encodes another DNA-binding hematopoietic transcription factor TAL1/SCL, known to be an Eklf activator and essential for definitive erythroid differentiation. Further identification of the authentic Tal gene promoter in combination with the in vivo genomic footprinting approach and DNA reporter assay demonstrate that EKLF activates the Tal gene through binding to a specific CACCC motif located in its promoter. These data establish the existence of a previously unknow positive regulatory feedback loop between two DNA-binding hematopoietic transcription factors, which sustains mammalian erythropoiesis. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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