Cell-specific expression of lung disease risk-related genes in the human small airway epithelium
| العنوان: | Cell-specific expression of lung disease risk-related genes in the human small airway epithelium |
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| المؤلفون: | M.J. Thomas, Sarah L. O’Beirne, Yael Strulovici-Barel, Juergen Schymeinsky, Philip L. Leopold, Mahboubeh Rostami, Robert J. Kaner, Wu-Lin Zuo, Michelle G. LeBlanc, Karsten Quast, Jay S. Fine, Sudha Visvanathan, Ronald G. Crystal, Jacqueline Salit, Shushila A. Shenoy, Jason G. Mezey |
| المصدر: | Respiratory Research Respiratory Research, Vol 21, Iss 1, Pp 1-11 (2020) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Lung Diseases, Lung Neoplasms, Cell, Gene Expression, Respiratory Mucosa, Epithelial cells, Cigarette Smoking, Lung Disorder, Pathogenesis, Transcriptome, 03 medical and health sciences, Idiopathic pulmonary fibrosis, Pulmonary Disease, Chronic Obstructive, 0302 clinical medicine, Immune system, Immune/inflammatory cells, Bronchoscopy, medicine, Inherited and acquired pulmonary disorders, Humans, Single-cell transcriptomes, Genetic Testing, lcsh:RC705-779, Lung, business.industry, Sequence Analysis, RNA, Research, lcsh:Diseases of the respiratory system, medicine.disease, 030104 developmental biology, medicine.anatomical_structure, 030228 respiratory system, Immunology, Respiratory epithelium, Airway Remodeling, business |
| الوصف: | Background The human small airway epithelium (SAE) plays a central role in the early events in the pathogenesis of most inherited and acquired lung disorders. Little is known about the molecular phenotypes of the specific cell populations comprising the SAE in humans, and the contribution of SAE specific cell populations to the risk for lung diseases. Methods Drop-seq single-cell RNA-sequencing was used to characterize the transcriptome of single cells from human SAE of nonsmokers and smokers by bronchoscopic brushing. Results Eleven distinct cell populations were identified, including major and rare epithelial cells, and immune/inflammatory cells. There was cell type-specific expression of genes relevant to the risk of the inherited pulmonary disorders, genes associated with risk of chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis and (non-mutated) driver genes for lung cancers. Cigarette smoking significantly altered the cell type-specific transcriptomes and disease risk-related genes. Conclusions This data provides new insights into the possible contribution of specific lung cells to the pathogenesis of lung disorders. |
| تدمد: | 1465-993X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e142d135aedbd337827f4dd36b60520aTest https://pubmed.ncbi.nlm.nih.gov/32727470Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e142d135aedbd337827f4dd36b60520a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1465993X |
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