Vascular and Blood Compatibility of Engineered Cationic Cellulose Nanocrystals in Cell-Based Assays
| العنوان: | Vascular and Blood Compatibility of Engineered Cationic Cellulose Nanocrystals in Cell-Based Assays |
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| المؤلفون: | Tanner Tobias, Beza Tuga, Alexandre Bernier, Yusha Imtiaz, Shreshth Kumar, Christopher W. Smith, Hoang Nguyen, Karina Ckless, Rajesh Sunasee |
| المصدر: | Nanomaterials, Vol 11, Iss 2072, p 2072 (2021) Nanomaterials Volume 11 Issue 8 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Chemistry, General Chemical Engineering, Cell, Cationic polymerization, Nanoparticle, immunomodulator, medicine.disease, Umbilical vein, Hemolysis, Article, Nanomaterials, medicine.anatomical_structure, medicine, Biophysics, cytotoxicity, General Materials Science, Viability assay, cationic, hemolysis, Cytotoxicity, QD1-999, cellulose nanocrystals |
| الوصف: | An emerging interest regarding nanoparticles (NPs) concerns their potential immunomodulatory and pro-inflammatory activities, as well as their impact in the circulatory system. These biological activities of NPs can be related to the intensity and type of the responses, which can raise concerns about adverse side effects and limit the biomedical applicability of these nanomaterials. Therefore, the purpose of this study was to investigate the impact of a library of cationic cellulose nanocrystals (CNCs) in the human blood and endothelial cells using cell-based assays. First, we evaluated whether the cationic CNCs would cause hemolysis and aggregation or alteration on the morphology of red blood cells (RBC). We observed that although these nanomaterials did not alter RBC morphology or cause aggregation, at 24 h exposure, a mild hemolysis was detected mainly with unmodified CNCs. Then, we analyzed the effect of various concentrations of CNCs on the cell viability of human umbilical vein endothelial cells (HUVECs) in a time-dependent manner. None of the cationic CNCs caused a dose-response decrease in the cell viability of HUVEC at 24 h or 48 h of exposure. The findings of this study, together with the immunomodulatory properties of these cationic CNCs previously published, support the development of engineered cationic CNCs for biomedical applications, in particular as vaccine nanoadjuvants. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2079-4991 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b0b4cad9f7f7ed1c2c7e988040a79e2Test https://www.mdpi.com/2079-4991/11/8/2072Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4b0b4cad9f7f7ed1c2c7e988040a79e2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20794991 |
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