دورية أكاديمية
Concentration-dependent effect of bleaching agents on the immunolabelling of interleukin-6, interleukin-17 and CD5-positive cells in the dental pulp
| العنوان: | Concentration-dependent effect of bleaching agents on the immunolabelling of interleukin-6, interleukin-17 and CD5-positive cells in the dental pulp |
|---|---|
| المؤلفون: | Benetti, F., Gomes-Filho, J. E., Ferreira, L. L., Sivieri-Araújo, G., Ervolino, E., Briso, A. L.F., Cintra, L. T.A. |
| المساهمون: | Universidade Estadual Paulista (UNESP) |
| سنة النشر: | 2018 |
| المجموعة: | Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
| مصطلحات موضوعية: | CD5, dental pulp, hydrogen peroxide, interleukin-17, interleukin-6, tooth bleaching |
| الوصف: | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) ; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) ; Processo FAPESP: 2013/25429-0 ; CNPq: 455943/2014-1 ; Aim: To evaluate lymphocyte-like cell activation (CD5-positive cells) and the expression of interleukin (IL)-6 and IL-17 in the pulp after tooth bleaching with two concentrations of hydrogen peroxide (H2O2). Methodology: The right and left maxillary molars from 40 rats were treated randomly with bleaching gel with 20% H2O2 (BLUE group, 1 application of 50 min), 35% H2O2 (MAXX group, three applications of 15 min), or placebo gel (control). After 2 and 30 days, the rats were killed (n = 10), and the jaws were processed for histological and immunohistochemistry analysis of the pulp tissue. The scores of inflammation and immunolabelling (IL-6/IL-17) were submitted to Mann–Whitney and Kruskal–Wallis followed Dunn tests, respectively; anova tests were used for comparisons of number of CD5-positive cells and pulp chamber area values (P < 0.05). Results: At 2 days, 60% of specimens of the BLUE group were associated with moderate inflammation in pulp horns, and in the MAXX group with necrosis (P < 0.05). At 30 days, the pulp was organized, and tertiary dentine was formed. The MAXX group had superior immunolabelling of IL-17 at 2 days differing significantly from other groups (P < 0.05). At 2 days, 90% of the specimens of the BLUE group had moderate immunolabelling of IL-6, and 50% of the MAXX group had severe immunolabelling, both significantly different from the control (P < 0.05). There was no significant difference between the groups at 30 days (P > 0.05). CD5-positive cells were present at 2 and 30 days, particularly in the bleached groups (P < 0.05), without significant difference between time periods (P > 0.05). Conclusions: IL-6 and IL-17 participated in inflammation in the pulp tissue of rats after tooth bleaching, particularly at 2 days. The immunolabelling was greater with increasing H2O2 concentration. ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 789-799 |
| اللغة: | English |
| تدمد: | 1365-2591 0143-2885 |
| العلاقة: | International Endodontic Journal; 1,791; http://dx.doi.org/10.1111/iej.12891Test; International Endodontic Journal, v. 51, n. 7, p. 789-799, 2018.; http://hdl.handle.net/11449/170673Test; 2-s2.0-85042111424; 4408095517346846; orcid:0000-0003-4859-0583 |
| DOI: | 10.1111/iej.12891 |
| الإتاحة: | https://doi.org/10.1111/iej.12891Test http://hdl.handle.net/11449/170673Test |
| حقوق: | closedAccess |
| رقم الانضمام: | edsbas.A9F6778A |
| قاعدة البيانات: | BASE |
| تدمد: | 13652591 01432885 |
|---|---|
| DOI: | 10.1111/iej.12891 |