Pak1 Kinase Promotes Activated T Cell Trafficking by Regulating the Expression of L-Selectin and CCR7
| العنوان: | Pak1 Kinase Promotes Activated T Cell Trafficking by Regulating the Expression of L-Selectin and CCR7 |
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| المؤلفون: | Lawrence E. Samelson, Roberto Weigert, Nicolas Melis, Bhagawat C. Subramanian, Ana Dios-Esponera |
| المصدر: | Frontiers in Immunology, Vol 10 (2019) Frontiers in Immunology |
| بيانات النشر: | Frontiers Media SA, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | lcsh:Immunologic diseases. Allergy, CD4-Positive T-Lymphocytes, 0301 basic medicine, Receptors, CCR7, Cell type, Transcription, Genetic, T-Lymphocytes, Lymphocyte, T cell, lymph node trafficking, Immunology, Pak1 kinase, Lymphocyte Activation, Mice, 03 medical and health sciences, 0302 clinical medicine, Cell Movement, medicine, Animals, Immunology and Allergy, L-Selectin, Lymphocyte homing receptor, Original Research, Mice, Knockout, biology, Forkhead Box Protein O1, Chemistry, JNK Mitogen-Activated Protein Kinases, Acquired immune system, Actin cytoskeleton, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Lymphatic system, Gene Expression Regulation, p21-Activated Kinases, L-selectin shedding, biology.protein, L-selectin, Lymph Nodes, lcsh:RC581-607, CCR7, Signal Transduction, 030215 immunology |
| الوصف: | Normal function of the adaptive immune system requires trafficking of T cells between the blood and lymphoid organs. Lymphocyte homing to lymph nodes requires that they cross endothelial barriers present in blood vessels and lymphatics. This multi-step process requires a remodeling of the lymphocyte plasma membrane, which is mediated by the dynamic re-arrangement of the actin cytoskeleton. Pak1 plays a central role in cell morphology, adhesion and migration in various cell types. Here we demonstrate that Pak1 is required for activated CD4+ T cell trafficking to lymph nodes. Pak1 deficiency in T cells causes a defect in the transcription of CCR7 and L-selectin, thereby altering lymphocyte trafficking. Additionally, we report an increase in L-selectin shedding in Pak1-deficient T cells, which correlates with a decrease in the recruitment of calmodulin to the cytoplasmic tail of L-selectin during T cell activation. Overall, our findings demonstrate that by regulating the expression of two major lymph node homing molecules, L-selectin and CCR7, Pak1 mediates activated CD4+ T cell trafficking. |
| تدمد: | 1664-3224 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d017309408bb93506a3304cb78371d73Test https://doi.org/10.3389/fimmu.2019.00370Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d017309408bb93506a3304cb78371d73 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16643224 |
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